CYTOTOXICITY OF STEM EXTRACTS OF SELECTED CASSIA SPECIES AGAINST HELA AND BREAST CANCER CELL LINES IN VITRO

Surekha R Deshpande; SHANKAR  NAIK B

doi:10.22159/ajpcr.2017.v10i3.11991

Authors

Surekha R Deshpande
SHANKAR NAIK B Dept. of BiologyGovt. Science collegeChikmagalur KarnatakaIndia 577101

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i3.11991

Abstract

ABSTRACT
Objective: The use of plants for medicinal remedies is an integral part of the Indian cultural life, and the traditional background of Indian medicine
shows widespread use of plant products in cancer treatment. In this study, stem extracts of some selected Cassia species have been evaluated for their
cytotoxic activities under in vitro conditions.
Methods: The stems were shade dried at room temperature. The dried and coarsely powdered plant material were extracted with petroleum ether
(60-80Â°C), chloroform, and ethanol using soxhlet apparatus. The cytotoxicity was evaluated by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide] assay.
Results: Stem extracts of three Cassia species, viz., Cassia glauca, Cassia Obtusifolia, and Cassia sophera have been evaluated for their cytotoxic activities
with chloroform, ethanol and pet ether against HeLa and breast cancer cell lines. Among the three different solvents used at different concentrations,
the chloroform extracts of all Cassia species exhibited maximum cytotoxicity (%) against both cell lines. The CTC50 values are revealed the cytotoxic
potential of C. glauca chloroform extracts against HeLa cell line and breast cancer cell lines with CTC50 values 180.00Â±3.0 and 146.67Â±0.5, respectively.
Chloroform extracts of C. Obtusifolia and C. sophera showed maximum activity against HeLa (380.00Â±1.1 and 800.00Â±1.7, respectively) and breast
cancer cell lines (310.00Â±1.1 and 633.33Â±0.6).
Conclusion: The results of this study demonstrate the potent cytotoxic activity of chloroform extracts of stems of Cassia species against HeLa and
Breast cancer cell lines.
Keywords: Cassia, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Cytotoxicity, HeLa, Breast cancer.

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Author Biography

SHANKAR NAIK B, Dept. of BiologyGovt. Science collegeChikmagalur KarnatakaIndia 577101

Dept of Biology

Proffessor

References

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