EVALUATION OF IN-VITRO CYTOTOXIC ACTIVITY OF CHLOROFORM EXTRACT OF SIDA ACUTA BURM.F.

Abstract

ABSTRACT

Objective: The aim of the present work was to investigate the in-vitro cytotoxic activity of chloroform extract of Sida acuta Burm.f.

Methods: The in-vitro cytotoxic activity of chloroform extract was performed by MTT assay method against A-431 (Human epidermoid carcinoma) and HeLa (Human cervical cancer) cell lines.

Results: The various concentration of crude chloroform extract (1000, 500, 250, 125, 62.5 µg/ml concentration) of Sida acuta whole plant was performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against A-431 cell lines (human epidermoid carcinoma) with an of 375±0.00 and HeLa cell lines (human cervix carcinoma with an IC₅₀ (inhibit cell growth by 50%) 610.00±2.5. The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the extract indicates that Sida acuta has anticancer activity against A-431-Human epidermoid carcinoma, HeLa-Human cervical cancer cell lines.

Conclusion: The present study concluded that the chloroform extract of Sida acuta Burm.f. posses potent cytotoxic activity.

Keywords: Sida acuta Burm.f., Chloroform extract, Cytotoxic activity, IC_50.