EFFECTIVITY AND PHYSICOCHEMICAL STABILITY OF NANOSTRUCTURED LIPID CARRIER COENZYME Q10 IN DIFFERENT RATIO of LIPID ALFA CETYL PALMITATE AND ALPHA TOCOPHERYL ACETATE AS CARRIER
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.14835Abstract
Objective: The aim of this study was to investigate physical characteristics of nanostructured lipid carriers (NLCs) mixture of alpha tocopheryl acetate, cetyl palmitate, Tween 80 and propylene glycol using high shear homogenization technique on NLC preparation to predict the optimum ratio of alpha tocopheryl acetate-cetyl palmitate to produce good characteristics of NLC loaded coenzyme, higher % EE, good penetration, controlled release, and stable.
Methods: Lipid characterizations were conducted by diffraction scanning calorimetry, X-ray diffraction, and Fourier transforms infrared spectrophotometry. Coenzyme Q10 concentration was measured by spectrophotometer at 275 nm. NLC characteristics based on their morphology was determined using transmission electron microscope, particle size, and its polydispersity index which were measured with Delsa Nanoâ„¢ particle size analyzer. Percentage of coenzyme Q10 entrapped in NLC was determined by dialysis bag method. Coenzyme Q10 release profile was measured using with Franz cell for 12 hrs. The penetration depth of NLC coenzyme Q10 in abdominal skin of Wistar rat was determined with fluorescence microscopy using rhodamine B as marker. NLC physical stability based on minimum of particle size variation, pH and viscosity during 90 days storage.
Results: The result showed that formula with ratio of cetyl palmitate-alpha tocopheryl acetate 70:30 (% w/w) produce good characteristics of NLC
loaded coenzyme, higher % EE, good penetration, controlled release, and stable in 90 days storage.
Conclusion: The coenzyme Q10 NLC system with cetyl palmitate and alpha tocopherol acetate as lipid matrixare characterized by small particle size, low crystallinity, spherical morphology of particle and high coenzyme Q10 entrapment efficiency. Crystal modification led to the formation of a more amorphous thereby increasing the drug entrapment
Keywords: Coenzyme Q10, Nanostructured lipid carrier, Cetyl palmitate, Alpha tocopheryl acetate, High shear homogenization.
Downloads
References
Hargreaves IP. Coenzyme Q10 as a therapy for mitochondrial disease.
Int J Biochem Cell Biol 2014;49:105-11.
Kovacevic A, Savic S, Vuleta G, Müller RH, Keck CM. Polyhydroxy
surfactants for the formulation of lipid nanoparticles (SLN and NLC):
Effects on size, physical stability and particle matrix structure. Int J Pharm 2011;406(1-2):163-72.
López-GarcÃa R, Ganem-Rondero A. Solid lipid nanoparticles (SLN) and
nanostructured lipid carriers (NLC): Occlusive effect and penetration
enhancement ability. J Cosmet Dermatol Sci Appl 2015;5:62-72.
Loo CH, Basri M, Ismail R, Lau HL, Tejo BA, Kanthimathi MS, et al.
Effect of compositions in nanostructured lipid carriers (NLC) on skin
hydration and occlusion. Int J Nanomedicine 2013;8:13-22.
Brugè F, Damiani E, Puglia C, Offerta A, Armeni T, Littarru GP,
et al. Nanostructured lipid carriers loaded with Coq10: Effect on
human dermal fibroblasts under normal and UVA-mediated oxidative
conditions. Int J Pharm 2013;455(1-2):348-56.
Fan H, Liu G, Huang Y, Li Y, Xia Q. Development of a nanostructured
lipid carrier formulation for increasing photo-stability and water
solubility of phenylethyl resorcinol. Appl Surf Sci 2014;288:193-200.
Agmo Hernández V, Eriksson EK, Edwards K. Ubiquinone-10 alters
mechanical properties and increases stability of 2 phospholipid
membranes. Biochim Biophys Acta 2015;1848:2233-43.
Pardeike J, Schwabe K, Müller RH. Influence of nanostructured lipid
carriers (NLC) on the physical properties of the Cutanova Nanorepair
Q10 cream and the in vivo skin hydration effect. Int J Pharm
;396(1-2):166-73.
Almeida MM, Bou-Chacra NA, Conte JD, Kaneko TM,
Baby AR, Velasco MV. Evaluation of physical and chemical stability
of nanostructured lipid carries containing ursolic acid in cosmetic
formulation. J Appl Pharm Sci 2013;3(01):005-8.
Castelli F, Puglia C, Sarpietro MG, Rizza L, Bonina F. Characterization
of indomethacin-loaded lipid nanoparticles by differential scanning
calorimetry. Int J Pharm 2005;304(1-2):231-8.
Piao H, Ouyang M, Xia D, Quan P, Xiao W, Song Y, et al. In vitro–in
vivo study of CoQ10-loaded lipid nanoparticles in comparison with
nanocrystals. Int J Pharm 2011;419(1-2):255-9.
Teeranachaideekul V, Souto EB, Junyaprasert VB, Muller RH.
Cetyl palmitate-based NLC for topical delivery of Coenzyme Q10 –
Development, physicochemical characterization and in vitro release
studies. Eur J Pharm Biopharm 2007;67(1):141-8.
Gupta RB. Fundamentals of Drug Nanoparticles in Nanoparticle
Technology for Drug Delivery. Vol. 159. New York: Taylor and Francis;
p. 124-30.
Lucangioli S, Tripodi V. The importance of the formulation in the
effectiveness of coenzyme Q10 supplementation in mitochondrial
disease therapy. Der Pharm Sin 2012;3(4):406-7.
Teeranachaideekul V, Boonme P, Souto EB, Müller RH,
Junyaprasert VB. Influence of oil content on physicochemical
properties and skin distribution of Nile red-loaded NLC. J Control
Release 2008;128(2):134-41.
Yue Y, Zhou H, Liu G, Li Y, Yan Z, Duan M. The advantages of a
novel CoQ10 delivery system in skin photo-protection. Int J Pharm
;392(1-2):57-63.
Zhai Y, Xiaoye Y, Zhao L, Wang Z, Zhai G. Lipid nanocapsules for
transdermal delivery of ropivacaine: In vitro and in vivo evaluation. Int
J Pharm 2014;471(1-2):103-11.
Korkm E, Gokce EH, Ozer O. Development and evaluation of
coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced
dermal delivery. Acta Pharm 2013;63(4):517-29.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
