IN VITRO CYTOTOXIC AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES ON ORTHOSIPHON STAMINEUS BENTH. (LEAF) AGAINST MCF–7 CELL LINES
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i3.15575Abstract
ABSTRACT
Objective: The objective of the study was to determine the anticancer efficacy of Orthosiphon stamineus extract against Michigan Cancer Foundation-7
(MCF-7) and its phytochemical analysis through gas chromatography-mass spectrometry (GC-MS).
Methods: Different solvents were used for leaf extraction and used for qualitative assay of phytochemicals using standard protocols. Different
concentration (12.5, 25, 50, 100, and 200 μg/ml) of methanol extract and ethyl acetate extract of O. stamineus leaves were used to assess the in vitro
cytotoxic effect using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the ethyl acetate extract was subjected to
GC-MS analysis, and the identification of components was based on the National Institute of Standards and Technology library database.
Result: Of the hexane, methanol and ethyl acetate extracts, methanol extract found to contain more of phytochemicals followed by ethyl acetate. The
inhibitory concentration 50 for methanol extract and ethyl acetate extract was found be 93.42 μg/ml and 215.3 μg/ml, respectively.
GC-MS mass spectrum of ethyl acetate extract revealed the presence of squalene and phytol and antioxidants such as flavones. Sterols compounds
such as vitamin E, ergosterol, cholesterol, and 3,7,11,15-tetramethyl-2-hexadecen-1-ol and terpenoids were also identified.
Conclusion: The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the results
further depicted that the selected traditional anti-cancer plants could be used not only as an anti-cancer but also as a good antioxidant.
Keywords: Anti-cancer, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Orthosiphon stamineus, Michigan cancer fsoundation-7
cells.
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