PROTECTIVE EFFECT OF BEE PROPOLIS AGAINST ANTI-TUBERCULOSIS DRUGS (RIFAMPICIN AND ISONIAZID)-INDUCED HEMATOLOGICAL TOXICITY IN SPRAGUE DAWLEY RATS

Umesh Bharti; Neelima R Kumar; Jaspreet Kaur

doi:10.22159/ajpcr.2017.v10i3.15991

Authors

Umesh Bharti pggcg-11,panjab university, chandigarh
Neelima R Kumar professor, Department of Zoology, Panjab university, Chandigarh, India
Jaspreet Kaur UIET,Panjab university

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i3.15991

Abstract

ABSTRACT
Objective: Protective effect of bee propolis against anti-tuberculosis (TB) drugs (rifampicin and isoniazid)-induced hematological toxicity in Sprague
Dawley (SD) rats.
Methods: Experimental male SD rats weighing 180Â±20 g were randomly assigned into eight groups (n=6), the Group 1 served as control; Group 2
received 200 mg bee propolis/kg body weight; Groups 3, 5, and 7 were treated with drugs 100 mg rifampicin/kg body weight, 50 mg isoniazid/kg
body weight, and 100 mg rifampicin+50 mg isoniazid/kg body weight, respectively. Groups 4, 6, and 8 were treatment groups receiving 200 mg bee
propolis/kg body weight+100 mg rifampicin/kg body weight, 200 mg bee propolis/kg body weight+50 mg isoniazid/kg body weight, and 200 mg bee
propolis/kg body weight+100 mg rifampicin+50 mg isoniazid/kg body weight, respectively. All the treatments were given for 30 days, and then, the
rats were sacrificed under light esthesia by cervical dislocation and blood was collected for physiological studies.
Results: Bee propolis supplementation (200 mg/kg body weight) showed increased level of hemoglobin with respect to rifampicin (15.45%),
isoniazid (11.34%), and rifampicin plus isoniazid (5.04%) administered groups after 30 days of treatment. Moreover, the decreased level of red blood
cell count and white blood cell count by anti-TB drugs rifampicin, isoniazid, and rifampicin plus isoniazid together was also elevated in treatment
group with bee propolis.
Conclusion: Coadministration of propolis (200 mg bee propolis/kg body weight) with drugs helped modulate the toxic effects by restoring tested
values to near normal.
Keywords: Propolis, Rifampicin, Isoniazid, Hemoglobin, Red blood cell count, White blood cell count.

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Author Biographies

Umesh Bharti, pggcg-11,panjab university, chandigarh

zoology department,post graduate govt. college for girls,sector-11, Chandigarh ,India

Neelima R Kumar, professor, Department of Zoology, Panjab university, Chandigarh, India

Zoology department, Professor

Jaspreet Kaur, UIET,Panjab university

Department of biochemistry, Uiet, Panjab university

References

REFERENCES

Banskota AH, Tezuka Y, Kadota S. Recent progress in pharmacological research of propolis. Phytother Res 2001;15(7):561-71.

Tasduq SA, Kaisar P, Gupta DK, Kapahi BK, Jyotsna S, Maheshwari HS, et al. Protective effect of a 50% hydroalcoholic fruit extract of Emblica officinalis against anti-tuberculosis drugs induced liver toxicity. Phytother Res 2005;19(3):193-7.

Rajeshkumar S, Kayalvizhi D. Antioxidant and hepatoprotective effect of aqueous and ethanolic extracts of important medicinal plant Pongamiapinnata (Family: Leguminoseae). Asian J Pharm Clin Res 2015;8(5):67-70.

WHO. Global Tuberculosis Report 2015. Geneva: WHO; 2015.

Schaberg T, Rebhan K, Lode H. Risk factors for side-effects of isoniazid, rifampicin and pyrazinamide in patients hospitalized for pulmonary tuberculosis. Eur Respir J 1996;9(10):2026-30.

Baciewicz AM, Chrisman CR, Finch CK, Self TH. Update on rifampin and rifabutin drug interactions. Am J Med Sci 2008;335(2):126-36.

Vijayalakshmi A, Thanmayi G, Jayakumari S. A prospective study on abnormal liver function test patterns in patients receiving anti-tuberculosis therapy. Asian J Pharm Clin Res 2016;9(5):136-9.

Bharti U, Neelima RK, Kaur J. Ameliorating effect of bee pollen against anti-tuberculosis drugs (rifampicin and isoniazid) induced toxicity on haematology of Sprague Dawley rats. Int J Sci Res 2016;5(4):2077-82.

Yee D, Valiquette C, Pelletier M, Parisien I, Rocher I, Menzies D. Incidence of serious side effects from first-line antituberculosis drugs among patients treated for active tuberculosis. Am J Respir Crit Care Med 2003;167(11):1472-7.

Eminzade S, Uras F, Izzettin FV. Silymarin protects liver against toxic effects of anti- tuberculosis drugs in experimental animals. Nutr Metab(Lond) 2008;5:1-8.

Ubaid RS, Anantrao KM, Jaju JB, Mateenuddin M. Effect of Ocimum sanctum (OS) leaf extract on hepatotoxicity induced by antitubercular drugs in rats. Indian J Physiol Pharmacol 2003;47(4):465-70.

Nasiru A, Hafsat IG, Sabo AA. Effect of anti- tuberculosis drugs co-administered with garlic homogenate on rat liver enzymes. IPCBEE 2012;40:96-8.

Kalia P, Kumar NR, Harjai K. Phytochemical screening and antibacterial activity of different extracts of propolis. Int J Pharm Biol Res 2013;3(6):219-22.

Youdim KA, Shukitt-Hale B, Mackinnon S, Kalt W, Joseph JA. Polyphenolics enhance red blood cell resistance to oxidative stress: In vitro and in vivo. Biochim Biophys Acta 2000;1523:117-22.

OrÅ¡oliÄ‡ N, Sirovina D, KonÄiÄ‡ MZ, LackoviÄ‡ G, GregoroviÄ‡ G. Effect of Croatian propolis on diabetic nephropathy and liver toxicity in mice. BMC Complement Alternat Med 2012;12:117.

Basnet P, Matsuno T, Neidlein R. Potent free radical scavenging activity of propol isolated from Brazilian propolis. Z Naturforsch C 1997;52(11-12):828-33.