SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA PRODUCING ENZYMES AGAINST ANTIMICROBIAL AGENT CELL FREE SUPERNATANT OF LACTOCOCCUS WITH THE FOCUS ON ITS DETERMINING QUANTITATIVELY BY OD (ENZYME LINKED IMMUNE SORBENT ASSAY)


Yusra Mb Mohsin, Huda Zuheir Majeed, Ali Murtatha Hasan, Enaam Hamed Bataah

Abstract


ABSTRACT
Objective: The present work was conducted to explore the prevalence of Pseudomonas aeruginosa that produce lipase and protease enzymes in
40 samples, as well as detection of quality and quantity production and determine its susceptibility to antimicrobial.
Methods: The antimicrobial activity of Lactococcus cell free supernatant (CFS) on P. aeruginosa growth and quantity production of enzymes by two
methods (special media and OD) were also studied.
Results: A total of 8(20%) and 13(32%) isolates were found to be positive to P. aeruginosa producing lipase and protease, respectively. The in vitro
antimicrobial activity results revealed that all isolates producing enzymes showed sensitive to CFS of Lactococcus, only one isolate exhibited low
sensitivity to CFS 4 mm, however, these isolates varied in their sensitivity to CFS ranged 4-15 mm.
Conclusion: Results of the quantity production of P. aeruginosa enzymes with CFS of Lactococcus showed and exerts growth inhibitory activity and
reduce the production of enzymes. We could be concluded that CFS of Lactococcus has great potential antimicrobial activity against P. aeruginosa
growth and its ability on enzymes production.
Keywords: Pseudomonas aeruginosa, Lactococcus, Cell-free supernatant, Optical density, Lipase, Protease.


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About this article

Title

SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA PRODUCING ENZYMES AGAINST ANTIMICROBIAL AGENT CELL FREE SUPERNATANT OF LACTOCOCCUS WITH THE FOCUS ON ITS DETERMINING QUANTITATIVELY BY OD (ENZYME LINKED IMMUNE SORBENT ASSAY)

DOI

10.22159/ajpcr.2017.v10i3.16188

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 291-294

Print ISSN

0974–2441

Online ISSN

2455-3891

Statistics

14 Views | 35 Downloads

Authors & Affiliations

Yusra Mb Mohsin
College of Veterinary Medicine
Iraq

Huda Zuheir Majeed

Ali Murtatha Hasan

Enaam Hamed Bataah


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