APOPTOSIS INDUCTION EFFECT OF CURCUMIN AND ITS ANALOGS PENTAGAMAVUNON-0 AND PENTAGAMAVUNON-1 ON CANCER CELL LINES


Muhammad Da'i, Andi Suhendi, Edi Meiyanto, Umar Anggoro Jenie, Masashi Kawaichi

Abstract


ABSTRACT
Objectives: This experiment aims to investigate the apoptosis effect of curcumin and its analogs pentagamavunon-0 (PGV-0) and PGV-1 on normal
and other cancer cell lines.
Methods: Growth inhibition effect was investigated using the MTT method. Double staining used acridine orange, 2-(4-aminodiphenyl)-6-
indolcarbamidine dihydrochloride and ethidium bromide was performed to determine morphological changes of cells. Detection of PARP, caspase-3,
PUMA and BAX using a western blot method was conducted to elucidate the apoptosis effect of the compounds.
Results: PGV-1 (2.5 μM) and PGV-0 (5.0 μM) could inhibit T47D-cell growth on 72 h observation, but not for curcumin. DNA staining showed PGV-1
has the strongest apoptosis induction effect on T47D-cells compared to PGV-0 and curcumin as well. Western blot analysis resulted in cleavage PARP
(83 kD) on HeLa, T47D, and MCF-7 cells treated with PGV-1 (2.5 μM), PGV-0 (5.0 μM). Curcumin (10.0 μM) just induced apoptosis on T47D-cell and
MCF-7 cell, but not HeLa cell. Cleavage PARP resulted by apoptosis process in the cell. PGV-1 (2.5 μM) had a stronger apoptosis effect compared to
PGV-0 (5.0 μM) and curcumin (10.0 μM) based on cleaved PARP result qualitatively. On the normal cell (NH3T3), cells that were treated with the
compounds resulted in a negative cleavage PARP. This result indicated that the compounds were part of a selectively induced cancer cell line apoptosis
process.
Conclusion: Curcumin, PGV-0 and PGV-1 could inhibit cell growth by induce apoptosis on cancer cells but not on normal cells, which PGV-1 has
strongest apoptosis induction effect on cancer cell lines.
Keywords: Curcumin and analogs, Apoptosis, Cancer cell lines.


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Fig. 3: Pentagamavunon-1 (2.5 μg/mL). (a) And PGV-0 (5.0 μg/mL), (b) induced apoptosis on HeLa, T47D and MCF-7. On higher concentration, both compounds could not induce apoptosis on NIH3T3 (normal cell) curcumin, (c) induced apoptosis on T47D and MCF-7 but not on HeLa and NIH3T3, (d) Further investigation of PGV-1 could activate caspase-3 on T47D-cells but not on HeLa and NIH3T3, increased PUMA and BAX on MCF-7 cell but not on HeLa and NIH3T3 cell

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Asian J Pharm Clin Res, Vol 10, Issue 3, 2017, 373-376

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About this article

Title

APOPTOSIS INDUCTION EFFECT OF CURCUMIN AND ITS ANALOGS PENTAGAMAVUNON-0 AND PENTAGAMAVUNON-1 ON CANCER CELL LINES

DOI

10.22159/ajpcr.2017.v10i3.16311

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 373-376

Print ISSN

0974–2441

Online ISSN

2455-3891

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Authors & Affiliations

Muhammad Da'i
Faculty of Pharmacy Universitas Muhammadiyah Surakarta
Indonesia

Andi Suhendi
Faculty of Pharmacy Universitas Muhammadiyah Surakarta
Indonesia

Edi Meiyanto
Universitas Gadjah Mada
Indonesia

Umar Anggoro Jenie
Faculty of pharmacy Universitas Gadjah Mada

Masashi Kawaichi
Nara Institute of Science and Technology, Ikoma, Japan
Japan

 

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