NON – RECEPTOR MEDIATED ANXIOLYSIS BY AMINO GUANIDINE: A NOVEL ACTIVITY AND SYNERGY WITH DIAZEPAM UNDER STRESSED CONDITIONS
Abstract
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Objective: A novel non-receptor mediated anxiolytic activity of amino guanidine (AG; 50 mg/kg, i.p.) was investigated and its possible interaction
with diazepam (DZP) (2 mg/kg, i.p.) was explored in mice.
Methods: Elevated plus maze and light/dark box were used to measure anxiety in Swiss albino mice. Plasma nitrite levels were estimated using Griess
reagent to observe the biochemical regulation of mice behavior.
Results: AG (50 mg/kg, i.p.) produced a significant antianxiety-like activity and significantly attenuated the plasma nitrite levels in stressed mice.
On the other hand, AG (50 mg/kg, i.p.) neither produced a significant antianxiety-like activity nor significantly altered the plasma nitrite levels in
unstressed mice. DZP (2 mg/kg, i.p.) produced a significant antianxiety-like activity in unstressed mice but not in stressed mice. DZP (2 mg/kg, i.p.)
did not significantly affect plasma nitrite levels in unstressed or stressed mice. AG and DZP, administered together produced a significant antianxietylike
activity in unstressed and stressed mice. However, combination of AG and DZP significantly attenuated the plasma nitrite levels in stressed mice
but not in unstressed mice.
Conclusion: These findings suggest a usefulness of a non-GABAergic mechanism of a potential anti-anxiety chemical that may avoid receptor-linked
undesired effects as in case of DZP and may be used with DZP under stressed conditions to serve the reduction of dosage of DZP.
Keywords: Amino guanidine, Anxiety, Diazepam, Stress.
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