INHIBITION OF ARACHIDONIC ACID-INDUCED EAR EDEMA, MAST-CELL STABILIZING AND FREE RADICAL EFFECT OF DICHLOROMETHANE CRUDE EXTRACTS FROM THE LEAVES OF LABISIA PUMILA

Patrick N. Okechukwu, S.O. Ekeuku (Views)

Abstract


Objective: To evaluate the ability of dichloromethane leave extract of labisia pumila (DELP) to  inhibit arachidonic acid - induced ear oedema, mast cell stabilizing effect and determination of DPPH,TPC, hydroxyl, nitric oxide and superoxide radical-scavenging activity of the extract.

Material and method: The leaf extract was prepared with dichloromethane filter and rotary evaporated and stored in desiccator. The effect of DELP on arachidonic acid - induced ear oedema, mast cell stabilizing was evaluated. DPPH,TPC, hydroxyl, nitric oxide and superoxide radical-scavenging activity of the extract was also studied.

Result: DELP was able to inhibit arachidonic acid induced ear edema in the mice. The concentration of DELP (mg/ml) needed for 50 percent  inhibition (IC50) of superoxide radicals, DPPH and NO were 1, 1.5, and 1.5 respectively. Total Phenolic Content was 25microgram GAE/mg.

 

Keywords— Anti-inflammation, antioxidant, ear-edema, Labisia pumila, mast-cell stabilization.


Full Text:

93-95

References


Lewis RA, Austen KF, Soberman RJ. Leukotrienes and other products of the 5-lipoxygenase pathway: biochemistry and relation to pathobiology in human diseases, N. Eng. J. Med. 1990: 323 645–655.

Tilley SL, Coffman TM, Koller BH. Mixed messages: modulation of inflammation and immune responses by prostaglandins and thromboxanes, J. Clin. Invest. 2001: 108 15–23.

Philip C. Polyunsaturated fatty acids and inflammatory processes: New twists in an old tale. Biochimie 2009: 91; 791–795.

Havsteen B. Flavonoids: a class of natural products of highpharmacological potency. Biochemical Pharmacology 1983: 32, 1141–1148.

Pathak D, Pathak K, Singla AK. Flavonoids as medicinal agents — recent advances. Fitoterapia 1991: 62, 371–389.

Tripathi RM, Sen PC, Das PK. Studies on the mechanism of action of Albizzialebbeck, an Indian indigenous drug used in the treatment of atopic allergy. Journal of Ethno pharmacology 1979:1,385–396.

Okechukwu PN, Samuagam L. The Study ofAntinociceptive Effects of MEOH and DCM Crude Extracts from the Leaves of Labisia pumila. Global journal of pharmacology 2011: 5 (3): 181-185, 2011

Inoue H, Mori T, Koshihara Y. Sulfidopeptides-leukotrienes are major mediators of arachidonic acid-induced mouse ear edema. Prostaglandins, 1988:vol.36, no. 5, pg. 731-739

Harish MS, Nagur NM, Badami S. Antihistaminic and mast cell stabilizing activity of Strigaorobanchioides. Journal of Ethnopharmacology 2001:76; 197–200.

Spanos GA, Wrolstad RE, Influence of processing and storage on the phenolic composition of Thompson seedless grape juice. JAgric food Chem, 1990: 38, 1565-1571.

Kikuzaki H, Nakatani N, Antioxidant effect of some ginger constitutents . J food Sc. 1993:58 1407-1410.

Garratt CJ. Effect of iodination on the biological activity of insulin. Nature, 1964: 201: 1324-1325.

Lavhale MS, Mishra SH. Evaluation of free radical scavenging activity of Butea monosperma Lam.Indian J Exp Biol 2007: 45(4), 376-84.

Young JM, Wagner BM Spires DA Tachyphlaxis 12-o-tetradecanoylphorbol acetate-and arachidonic acid-induced ear induced ear edema. J. Invest. Dermatol 1983: 80: 48

Young M, Spires DA, Bedord CJ. The mouse ear inflammatory response to topical arachidonic acid. J. Invest. Dermatol 1984: 8,2; 367.

Opas EE, Bonny RJ, Humes JL. Prostaglandin and leukotriene synthesis in mouse ears inflamed by arachidonic acid. J. Invest. Dermatol 1985:84: 253

Chang J, Carlsons RP, O’Neill-Davis L. Correlation between mouse skin inflammation induced by arachidonic acid and eicosanoid synthesis. Inflammation 1986: 10: 205.

Okechukwu PN , Ikujuni CM. The study of anti-inflammatory anti-pyretic and anti-nococeptive activities of extract from leaves of Labisia pumila. International Journal of Pharmacology & Toxicology Science 2011: 2(2), 12-27.

Subramoniam A, Evans DA, Valsaraj R. Inhibition of antigen-induced degranulation of sensitized mast cells by Trichopus zeylanicus in mice and rats. J. Ethnopharmacol. 1999:68,137–143.

Crossland J. Pharmacological Aspects of Allergy. Lewis Pharmacology. Churchill Livingstone, New York, 1980: p. 393.


Refbacks

  • There are currently no refbacks.