VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF BISOPROLOL FROM ITS BULK AND TABLET FORMULATION
The proposed method is new, simple, sensitive, reproducible, economical, accurate and precise and can be successfully applied in estimation of bisoprolol. This method can find applications in clinical studies and therapeutic drug monitoring. This method of colorimetric estimation of bisoprolol is based on the formation of blue colored chromogen when reacted with ferric chloride and pottasium ferricynide. The concentration of bisoprolol over range of 1- 13 micro g/ml was found to obey Beer’s law in the stated range. The blue colored complex has absorption maxmia at 770 nm with molar absorptivity and sandell’s 2.2387 x 104 lit mol-1 cm-1 and0.0154 micro g/cm2/0.001 absorbance units, reproducible, specific, and the reagent was not found to react with the soluble matters of the body fluids. The results analysis were validated as per ICH Q2B guidelines.
Keywords: Bisoprolol, visible spectroscopy, validation
Li Ding, Xia Zhou, XiaofengGuo, Qinxin Song, Jianchang He, GuiliXu. Lc-Esi-Ms method for The determination of bisoprolol in human plasma, J Pharm Biomed Anal, 2007; 44 (2) 302-305.
K Ding L, Zhou X, Guo X, Song Q, He J, Xu G. LC-ESI-MS Method for the determination of bisoprolol in human Plasma. J Pharm Biomed Anal, 2007; 28, 44(2), 520-525.
L J Patel, BnSuhagia, Pb Shah, Rr Shah. Simultaneous estimation of bisoprololfumarate and Hydrochlorothiazide in tablet dosage form by RP-HPLC method, Indian J. Pharma. Sci., 2006; 68(5), 635-638.
Patel L.J. et al. Simultaneous estimation of bisoprololfumarate and hydrochlorothiazide in tablet dosage form by RP-HPLC method. Indian J. Pharma. Sci., 2006; 68(5), 635-638.
Clarice M.B.R. Development and validation of an RPHPLC method for the dissolution studies of bisoprolol in pharmaceutical dosage form. J. Liq. Chromatogr. And Rel.Tech., 2005; 28, 477-486
CorneliuOniscu, Cristina VictorinaVlase, Gabriela Peste. A new high performance liquid chromatographic method for determination of bisoprolol in plasma samples, Roumanian Biotechnological Letters, 2007; 12 (1), 3079-3084.
M.D. Hernardo, M. Petrovic, A.R. Fernandez –A`Lba, d. barcelo. Analysis by liquid chromatography- electrospray ionization tandem mass spectrometry and acute toxicity evaluation for β –blockers and lipid-regulating agents in wastewater samples. Journal of Chromatography A, 2004; 1046, 133-140.
Shuijun Li, Gangyi Liu, JingyingJia, Yun Liu, Cheng Pan, Chen Yu, YongbaoCai, JianyingRen. Simultaneous determination of ten antiarrhythmics drugs and a metabolite in human plasma by liquid Chromatography- tandem mass spectrometry, Journal of Chromatography.B 2007; 847 (2) 174- 181.
Bhatt j., Subbaiah G., Kambli s., shah b., Patel M., Saxena A., Baliga A., Nigam S., Parekh H., Yadav G. A high throughput and sensitive liquid chromatography-tandem mass Spectrometry (LC-MMS/MS) method for the estimation of bisoprolol in human plasma using multiplex Technique”, Journal of Chromatography. B, 2007; 852 (1-2) 374-381.
Saleem Shaikha, O.A. Thusleema, M.S. Muneeraa, JaveedAkmala, Anand V. Kondagulia, K. Ruckmani. A Simple and rapid high-performance liquid Chromatographic method for the determination of Bisoprolol fumarate and hydrochlorothiazide in a Tablet dosage form, Journal of Pharmaceutical and Biomedical Analysis, 2008; 48: 1055–1057.
TakatoshiTakubo, Hiromasa Okada, Mikio Ishii, Kenichi Hara, Yasuyuki Ishii. Sensitive and selective liquid Chromatography–electrospray ionization tandem Mass spectrometry analysis of hydrochlorothiazide in Rat plasma, Journal of Chromatography B, 2004; 806, 199-203.
Myriam C. Salvadori, Roberto F. Moreira Bruno C. Borges Maristela H. Andraus Cristina P. Azevedo, Ronilson A. Moreno Ney C. Borges .Simultaneous Determination of Losartan and Hydrochlorothiazide in Human Plasma by LC/MS/MS with Electrospray Ionization and Its Application to Pharmacokinetics, Clinical and Experimental Hypertension, 2009; 31, 415-427.
Maha F. Tutunji, Husam M. Ibrahim, Manal H. Khabbas and Lara F. Tutunji. Simultaneous Determination of bisoprolol and hydrochlorothiazide in Human plasma by HPLC coupled with tandem mass Spectrometry, Journal of Chromatography B, 2009; 877.
Liu M, Zhang D, Sun Y, Wang Y, Liu Z, Gu J. Liquid Chromatographic-electrospray tandem mass Spectrometric determination of bisoprolol in human Plasma Biomed Chromatogr, 2007; 21, 508-513.
Sahu R. and Patel V.B. Simultaneous spectrophotometric estimation of hydrochlorothiazide and bisoprololfumarate in combined dosage forms. Indian J. Pharma. Sci, 2006; 68(6), 764-767.
K. Raghubabu, N. Mohan, B. Kalyanaramu, C. Ramdas, M. N. Rao. Development of new visible specrophotometric determination of tiaprofenic Acid in bulk and formulations, Asian J Pharm Clin Res, 2013; 6 (1), 77-79.
Aitha vijaya lakshmi. Spectrophotometric method for estimation of chloroquine in bulk and tablet dosage form, Asian J Pharm Clin Res, 2013; 6(1), 156-158.
- There are currently no refbacks.