ANTIOXIDANT POTENTIAL, TOTAL PHENOLIC AND TOTAL FLAVONOIDS CONTENT OF VARIOUS EXTRACTS FROM WHOLE PLANT OF POLYCARPAEA CORYMBOSA LAM.
Abstract
Objective: The objective of the study was to evaluate antioxidant potential, total phenolic and total flavonoids content of various extracts of P.corymbosa.
Methods: The antioxidant activity of various extracts of P.corymbosa were analysed by using DPPH assay, superoxide radical scavenging assay and total antioxidant activity method.
Results: The ethanolic extract of P.corymbosa had showed significant radical scavenging activity. Similar result was not observed in other two extracts. The higher content phenolic and flavonoids were found in ethanolic extract of P.corymbosa in comparison with other two extracts.
Conclusion: The results obtained from this study indicate that P.corymbosa is a potential source of antioxidants and thus could prevent many radical diseases.
Key words: P.corymbosa, antioxidant, phenols, flavonoids.
Downloads
References
Yu BP. Cellular defences against damage from reactive oxygen species. Physiol Rev. 1994; 74: 139.
Halliwell b and Gutteridge JMC. In Free radicals in biology and medicine, 2 nd ed (Clarendon press, Oxford) 1988; 1.
Lata H and Ahuja GK. Role of free radicals in health and disease. Ind J Physio and Allied Sci. 2003; 57: 124.
Beris H. Antioxidant effect a basis of drug selection. Drugs 1991; 42: 569-605.
Shivprasad HN, Mohan S, Kharya MD, Shiradkar MR, Lakshman K. In-Vitro Models For Antioxidant Activity Evaluation: A Review. Pharmaceutical Rev 2005; 3.
Campbell IC and Abdulla EM, Strategic approaches to in vitro neurotoxicology, in Approaches and methods: Neurotoxicology, (Academic Press, London) 1995;495.
Marx JL. Oxygen free radicals linked to many diseases. Science. 1987; 235: 529.
K Madhava chetty; K Sivaji ; K Tulasi Rao. Polycarpaea corymbosa L.†Flowering Plants of Chittoor District,Andhra Pradesh, India. 2008.
R. Varadharajan and D. Rajalingam. Hepatoprotective effect of Polycarpaea corymbosa l. against CCl4 induced hepatotoxicity in rats. Inter. J. of Pharmacotherapy , 2012; 2(1): 18-23.
Sindhu S and S Manorama. Antimicrobial activity of Polycarpaea corymbosa Lam. (Caryophyllaceae) against human pathogens. Journal of Chemical and Pharmaceutical Research, 2012; 4(8):4014-4019.
Harborne JB. Phytochemical methods 11 Edn. In Chapman &, Hall. New York, 1984: 4-5.
Mensor LL, Meneze FS, Leitao GG, Reis AS, Dos santor JC, Coube CS and Leitao SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother.Res, 2001; 15: 127-130.
Winterbourne CC, Hawkins RE, Brain M and Carrel RW. The estimation of red cell superoxide dismutase activity. J. Lab.chem.Med, 1975; 85: 337-341.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E. Anal. Biochem, 1999; 269: 337-341.
Mallick CP and Singh MB. Plant enzymology and Histoenzymology (eds), Kalyani publishers, New Delhi, 1980; 286.
Cameron GR, Milton RF and Allen JW. Measurement of flavonoids in plant samples. Lancet, 1943; 179.
Molyneux P. The use of the stable free radical diphenyl picryl hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004;26:211-9.
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford: Oxford University Press; 1999.
Hatano T, Edamatsu R, Mori A, et al. Effect of interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chemical and Pharmaceutical Bulletin 1989; 37: 2016–2021.
Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebnesmittel-Wissenschaft und Technologie, 1999; 32: 269–277.
Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP and Rice Evans C, Polyphenolic flavonoids as scavengers of aqueous phase radicals and as chain-breaking antioxidants, Arch Biochem Biophys, 1995; 322(2): 339-346.
Van Acker SABE, Van den Vijgh WJF and Bast F, Structural aspects of antioxidant activity of flavonoids, Free Rad Bio Med, 1996; 20(3): 331-342.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.