COMPARATIVE FTIR ANALYSIS AND FREE RADICAL QUENCHING PROPERTIES OF THREE CASSIA SPECIES
Abstract
Objective: To evaluate the comparative Fourier Transform Infrared spectroscopic analysis and the in vitro antioxidant screening of leaf extract of
three Cassia species.
Methods: The 80% leaf methanol extract of all three species was tested for their total phenols, flavonoids, antioxidant screening (DPPH, ABTS, Nitric
oxide, radical reducing power and total antioxidant capacity) and along with spectroscopic study.
Results: The results revealed that C. uniflora had the highest total phenolic content (276.54±0.031 µg/ mg DW) followed by C. occidentalis and C. tora,
while the highest total flavonoid content (43.71±0.086 µg /mg DW) was also recorded to C. uniflora followed by C. tora and C. occidentalis. FTIR
screening revealed the presence of various functional groups such as aldehydes, alcohols, carboxylic acid, alkenes, aromatic compounds, esters, nitro
compounds and alkyl halides in all the three species. Overall all the species exhibited dose dependent antioxidant activity in tested in vitro models.
The antioxidant activity was varied to each species; the highest IC50 values for DPPH (58.55±0.213 μg/ml) were depicted in C. occidentalis followed
by C. tora and C. uniflora. The prominent IC50 values for ABTS radical scavenging was reported in C. uniflora extract (IC50value of 0.074±0.003 μg/ml)
followed by C. occidentalis and C. tora (with IC50 values of 24.388±0.183 and 29.388±0.368 μg/ml equivalent to ascorbic acid).
Conclusion: The results of the present study indicate that, of the Cassia species evaluated, C. occidentalis exhibited strong activity followed by C. tora
and C. uniflora, and can be used as a potent source of natural antioxidants.
Keywords: Cassia species, Leaf extract, Fourier transform infrared spectroscopy, Antioxidant activity, Ascorbic acid.
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