Comparative techniques for detecting mastitis disease in bovine milk samples

Amit Gupta

Abstract


Objective-The objective of our study is to analyze various techniques that are applied for detecting mastitis disease in milk samples.

Methods- In this study, we collect milk samples (n=100) from different dairy farms and diagnosed through various tests. In addition, flow cytometric analysis was also performed for milk samples in order to analyzed lymphocytes, monocytes and granulocytes count.

Results- The results of these studies showed that California mastitis Test (CMT) showed much more positive results against mastitis disease as compared to other techniques whereas flow cytometric results revealed that during mastitis disease, there is enhancement in granulocytes count.

Conclusion- Out of these techniques, CMT is the most reliable and cost effective method for detecting mastitis disease.


Full Text:

PDF

References


REFERENCES

Barbosa-Cesnik C, Schwartz K and Foxman B. Lactation mastitis. JAMA 2003; 289 (13): 1609–1612.

Blum S, Heller ED, Krifucks O, et al. Identification of a bovine mastitis Escherichia coli subset. Vet Microbiol 2008; 132:135–148.

Jayarao BM, Pillai SR, Sawant AA et al. Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. J Dairy Sci 2004; 87: 3561-3573.

Elhaig MM, Selim A. Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt. Trop Anim Health Prod 2015; 47(2): 271-276.

Selvaraju G, Geetha M, Saravanan S, Manicavasaka DA. Evaluation of indirect tests for screening subclinical mastitis in dairy cows. Ind J Dairy Sci 2013; 66 (1): 55- 57.

Kumar GSN, Apannavar MM, Surnagi MD, Kotresh AM. Study on incidence and economics of clinical mastitis. Karnataka J Agricultural Sci 2010; 23 (2): 407-408.

Barker AR, Schrick FN, Lewis MJ, Dowlen HH, Oliver SP. Influence of clinical mastitis during early lactation on reproductive performance of Jersy cows. J Dairy Sci 1998; 81: 1285-1290.

Holdaway RJ, Holmes CW, Steffert IJ. A comparison of indirect methods for diagnosis of subclinical intramammary infection in lactating dairy cows. Aust. J. Dairy Tech. 1996; 51: 64 -71.

Chopade D, Gupta A, Kale P, Chaphalkar SR. Prevalence of bacteria from mastitis disease in dairy animals: Antibiotic sensitivity. Asian Journal of clinical research 2016; 3: 24-28.

Gupta A, Chopade D, Chaphalkar SR. Prevalence of bacterial pathogens in mastitis disease: Review. Asian Journal of clinical research 2016; 3: 8-11.

Leach KA, Green MJ, Breen JE, Huxley JN, Macaulay R, Newton HT, Bradley AJ. Use of domestic detergents in the California mastitis test for high somatic cell counts in milk. Veterinary Record 2008; 163: 566-570.

Reddy BSS, Kumari KN, Reddy YR, Reddy MVB, Reddy BS. Comparison of Different Diagnostic Tests in Subclinical Mastitis in Dairy Cattle. Int J Veterinary Science 2014; 3(4): 224-228.

Fernando RS, Spahr SL, Fr EHJ. Comparison of electrical conductivity of milk with other indirect methods for detection of sub-clinical mastitis, J Dairy Sci 1985; 68:440


Refbacks

  • There are currently no refbacks.