COMPARATIVE COMPOSITIONS AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OILS OF ANTHEMIS NOBILIS L. AND ANTHEMIS MIXTA L. (ASTERACEAE)
Keywords:
Anthemis nobilis L, Anthemis mixta L, chemical composition, antibacterial activityAbstract
Objective: To evaluate the chemical composition of essential oils from aerial parts of Anthemis nobilis (An) and Anthemis mixta L (Am). and investigate their antibacterial property.
Methods: The essential oils were isolated by hydro distillation and analyzed by GC and GC-MS. The disc diffusion and Agar dilution methods were used to screen the antibacterial activity against referenced and/or clinically isolated (HS) strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella pneumonia and Shighuelle sonnei.
Results: The main constituents of An essential oil were 3-methyl-2-buten-1-yl cyclopropane carboxylate (29.3%), vinyl-2,2-dimethylbutanoate (24.2%), glycidyl methacrylate (9.9%), 2-methylbutyl-2-mehylbutyrate (9.1%), isobutyl isobutyrate (7.8%) and 3,3,4-trimethylhexane (6.2%), while Am essential oil was mainly characterized by α-thujone (51.8%), β-thujone (14.6%), borneol (7.3%) and 3-hexen-1-ol (4.9%). An and Am essential oils exhibited the best antibacterial activity against the following strains of Escherichia coli ATCC 25922 (25±1.20, 32±1.00 mm), Pseudomonas aeruginosa ATCC 27853 (23±0.87, 32±0.76 mm) and Staphylococcus aureus ATCC 43300 (21±1.44, 25±1.24 mm inhibition zone diameters, respectively). Minimum inhibitory concentration (MICs) values of these oils were ranged from 32-128µg/ml.
Conclusion: From this study, it can be concluded that the chemical composition of the essential oils was related to their antibacterial activity.
Keywords: Anthemis nobilis L., Anthemis mixta L., Chemical composition, Antibacterial activity
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References
MartÃnez MJ, Betancourt J, Alonso-González N, Jauregui A. Screening of some Cuban medicinal plants for antimicrobial activity. J Ethnopharmacol 1996;52:171-7.
Mahasneh AMA, Adel MA, El-Oqlah AAB. Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan. J Ethnopharmacol 1999;64:271-6.
Navarro V, Villarreal ML, Rojas G, Lozoya X. Antimicrobial evaluation of some plants used in Mexican traditional medicine for the treatment of infectious diseases. J Ethnopharmacol 1996;53:143–7.
Rehder VLG, Machado ALM, Delarmelina C, Sartoratto A, Duarte MCT, Figueira GM. Composiçăo quÃmica e activida de antimicrobiana do óleo essential de duas espécies d’Origanum. Rev Bras Plantas Med 2004;6:67-71.
Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 2001;74:113-23.
Oberprieler C. Phylogenetic relationships in Anthemis L. (Compositae, Anthemideae) based on nrDNA ITS sequence variation. Taxon 2001;50:745-62.
Rossi T, Melegari M, Bianchio A, Albasini A, Vampa G, Sedative. Anti-inflammatory and antidiuretic effects induced in rats by essential oils of varieties of Anthemis nobilis: a comparative study. Pharmacol Res Commun 1988;20:71-4.
Boutaghane N, Kabouche A, El-Azzouny AM, Kabouche Z. The composition of the essential oil of Chrysanthemum macrocarpum from Algeria. Chem Nat Compd 2008;44:817-8.
Boutaghane N, Kabouche A, Touzani R, Maklad YA, El-Azzouny A, Bruneau C, et al. GC/MS analysis and analgesic effect of the essential oil of Matricaria pubescens from Algeria. Nat Prod Commun 2011;6:251-2.
Berhail Boudouda H, Kabouche A, Aburjai T, Kabouche Z. GC-MS analysis of Inula graveolens (L.) Desf. from Algeria. J Essent Oil Bear Plants 2013;16:651-4.
Chibani S, Gherboudj O, Kabouche A, Aburjai T, Touzani R, Kabouche Z. GC-MS analysis of Senecio giganteus Desf from Algeria. J Essent Oil Bear Plants 2013;6:123-5.
Tadrent W, Kabouche A, Touzani R, Kabouche Z. Chemotypes investigation of essential oils of Guertoufa†herbs. J Mater Environ Sci 2014;5:1200-5.
Tadrent W, Benteldjoune M, Laggoune S, Benmerache A, Kabouche A, Semra Z, et al. Composition and antibacterial activity of the essential oil of Cotula anthemoides L. (Asteraceae). Chem Nat Compd 2014;50:744-5.
British Pharmacopoeia, HMSO, London, UK; 1998. p. A137-8.
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 4th Ed. Illinois: Allured Publishing Co. Carol Stream; 2007.
Mclafferty FW, Stauffer DB. The important peak index of the registry of mass spectral data. New York: John Wiley and Son; 1991.
Villionova PA. NCCLS Performance standards for antimicrobial disk susceptibilities tests, USA: Approch Standard NCCLS Publication; 1993. p. M2-A5.
Radulovi NS, Blagojevi PD, Zlatkovi BK, Pali RM. Chemotaxonomically important volatiles of the genus Anthemis L–A detailed GC and GC/MS analyses of Anthemis segetalis Ten from montenegro. J Chin Chem Soc 2009;56:642-52.
Rossi T, Melegari M, Bianchi A, Albasini A, Vampa G. Sedative, anti-inflammatory and antidiuretic effects induced in rats by essential oils of varieties of Anthemis nobilis: a comparative study. Pharmacol Res Commun 1988;20:71-4.
Uzel A, Guvensen A, Cetin E. Chemical composition and antimicrobial activity of the essential oils of Anthemis xylopoda O Schwarz from turkey. J Ethnopharmacol 2004;95:151-4.
Nemeth E, Bernath J. Biological activities of yarrow species (Achillea spp.). Curr Pharm Des 2008;14:3151-67.