ANTIOXIDANT AND ANTIPROLIFERATIVE POTENTIALS OF MARINE ACTINOMYCETES
Keywords:
Marine actinomycetes, Antioxidant, Antiproliferative, MDA-MB 231Abstract
Objective: The aim of present studyis to isolate and characterize a novel actinomycetes strain from marine sediments collected at Kakinada coast. To evaluate antioxidant and antiproliferative activities of the crude methanolic extract of isolated actinomycetes.
Methods: Actinomycetes was isolated from marine sediments collected at Kakinada coast using starch casein agar. Morphological, biochemical and molecular characterization was performed and the crude methanolic extract was used for determination of in vitro antioxidant activity and antiproliferative activity against MDA-MB 231 breast cancer cells.
Results: The crude methanolic extract exhibited significant antioxidant activity with ICÂ50 value in the range of 30-46 µg/ml in different radical scavenging assays. Moreover, the extract showed concentration dependent cytotoxicity against triple negative breast cancer cell line, MDA-MB 231 with IC50 of 42.5µg/ml. The extract also inhibited cell proliferation with IC50 value 44.1 µg/ml probably by arresting cell cycle at S phase. The antioxidant activity of the extract was strongly correlated with cytotoxic and antiproliferative activity.
Conclusion: The isolated marine actinomycetes can be the potential source of antioxidants with the anticancer property.
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Mallikarjuna Rao T, Ganga Rao B, Venkateswara Rao Y. Antioxidant activity of Spilanthes acmella extracts. Int J Phytopharmacol 2012;3:216-20.
Wayner DDM, Burton GW, Ingold KU, Locke S. Quantitative measurement of the total, peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation. FEBS Lett 1985;187:33-7.
Arulpriya P, Lalitha P, Hemalatha S. In vitro antioxidant testing of the extracts of Samanea saman (Jacq). Merr Der Chem Sin 2010;1:73-9.
Halliwell B, Gutteridge J. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 1984;219:1-14.
Wee Sim Choo, Wee Khing Yong. Antioxidant properties of two species of Hylocereus fruits. Adv Appl Sci Res 2011;2:418-25.
Dhivya Balakrishnan, Dhevendran Kandasamy, Paramasivam Nithyanand. A review on antioxidant activity of marine organisms. Int J ChemTech Res 2014;6:3431-6.
Sithranga Boopathy N, Kathiresan K. Anticancer drugs from marine flora. J Oncol 2010. Doi.org/10.1155/2010/214186. [Article in Press]
Sambamurthy K, Ellaiah P. A new streptomycete producing neomycin (B and C) complex S. marinensis (Part I). Hind Antibiot Bull 1974;17:24–8.
Ellaiah P, Reddy APC. Isolation of actinomycetes from marine sediments of Visakhapatnam, east coast of India. Indian J Mar Sci 1987;16:134–5.
Ellaiah P, Kalyan D, Rao VSV, Rao BVLN. Isolation and characterization of bioactive actinomycetes from sediments. Hind Antiobiot Bull 1996;38:48–52.
Ellaiah P, Adinarayana K, Naveen Babu A, Thaer B, Srinivasulu T, Prabhakar T. Bioactive actinomycetes from marine sediments of bay of Bengal near machilipatnam. Geobios 2002;29:97–100.
Ellaiah P, Ramana T, Bapi Raju KVVSN, Sujatha P, Uma Sankar A. Investigations on marine actinomycetes from bay of Bengal near Kakinada coast of Andhra Pradesh. Asian J Microbiol Biotechnol Environ Sci 2004;6:53–6.
Gorajana A, Kurada BV, Peela S, Jangam P, Vinjamuri S, Poluri E, et al. 1-Hydroxy-1-norresistomycin, a new cytotoxic compound from a marine actinomycete,Streptomyces chibaensis AUBN1/7. A. J Antibiot 2005;58:526–9.
Manivasagan P, Gnanam S, Sivakumar K, Thangaradjou T, Vijayalakshmi S, Balasubramanian T. Antimicrobial and cytotoxic activities of an Actinobacteria (Streptomyces Sp. PM-32) isolated from an offshore sediments of the bay of Bengal in Tamilnadu. Adv Biol Res 2009;3:231-6.
Saha M, Jaisankar P, Das S, Sarkar KK, Roy S, Besra SE, et al. Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine actinobacteria sp isolated from the bay of Bengal. Biotechnol Lett 2006;28:1083-8.
Suthindhiran K, Kannabiran K. Diversity and exploration of bioactive actinomycetes in the bay of Bengal of the puducherry cost of India. Indian J Microbiol 2010;50:76–82.
Ravikumar S, Fredimoses M, Gnanadesigan M. Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines. Asian Pac J Trop Biomed 2012;2:92-6.
Salle AJ. Laboratory manual on fundamental principles of Bacteriology. 3rd Edn; 1939. p. 175.
Williams ST, Coss T. Actinomycetes isolation from soil. In: Methods in Microbio. Booth C. Ed. Academic Press: London, New York; 1971. p. 295-334.
Lampert Y, Kelman D, Dubinsky Z, Nitzan Y, Hill RT. Diversity of culturable bacteria in the mucus of the red sea coral Fungia scutaria. FEMS Microbiol Ecol 2006;58:99-108.
Rama Rao M, Bhaskar Reddy I, Raja Gopal SV, Naveen Kumar AD, Ramesh A, Kartika T. Effect of Holoptelea intigrifolia bark extract on radical scavenging and 5’-lipoxygease activities. Biomed Pharmacol J 2008;1:349-58.
Kumari S, Malla RR, Reddy IB, Rao VR. Study on radical scavenging activity and analysis of bioactive compounds in selected Indian medicinal plants. Biosci Biotech Res Asia 2010;72:913-8.
Liu F, Ooi VEC, Chang ST. Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci 1997;60:763-71.
Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powerâ€. The FRAP assay. Anal Biochem 1996;239:70-6.
Rana Abu-Dahab, Fatma Afifi, Violet Kasabri, Lara Majdalawi, Randa Naffa. Comparison of the antiproliferative activity of the crude ethanol extracts of nine salvia species grown in Jordan against breast cancer cell line models. Pharmacogn Mag 2012;8:319–24.
R Rao Malla, Raghu H, Rao JS. Regulation of NADPH oxidase (Nox2) by lipid rafts in breast carcinoma cells. Int J Oncol 2010;37:1483-93.
Sirisha B, Haritha R, Jagan Mohan YSYV, Siva Kumar. K, Ramana T. Bioactive compounds from marine Actinomycetes isolated from the sediments of bay of Bengal. Int J Pharm Chem Biol Sci 2013;3:257-64.
Mincer TJ, Jensen PR, Kauffman CA, Fenical W. Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl Environ Microbiol 2002; 68:5005–11.
Laidi, Rabah-Forar, Amany Kansoh, Ali L, Elshafei, Bengraa Cheikh M. Taxonomy, identification and biological activities of a novel isolate of Streptomyces tendae. Arab J Biotechnol 2006;9:427-36.
Sivakumar K, Sahu MK, Thangaradjou T, Kannan L. Research on marine actinobacteria in India. Indian J Microbiol 2007;47:186-96.
Ganesh Babu B, Naveen Kumar AD, Bhaskar Rao D, Laxmi koteswaramma K, Swarna P, Malla RR. Analysis of antioxidant and anticancer potentials of Urginea indica, an endangered medicinal plant. J Pharm Res 2012;5:4921-8.
Mohankumar Thenmozhi, Krishnan Kannabiran. Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7. Oxidants Antioxidants Med Sci 2012;1:51-7.
Arulappan Jayaprakash Priya, Elumalai Sagadevan, Perumal Dhanalakshmi, Sivaji Sathish Kumar, Vijayan Karthikeyan, Perumal Arumugam. Detection of antioxidant and antimicrobial activities in marine Actinomycetes isolated from puducherry coastal region. J Modern Biotechnol 2012;1:63–9.
Loganathan karthik, Gaurav kumar, Kokati Venkata Bhaskar Rao. Antioxidant activity of newly discovered lineage of marine actinobacteria. Asian Pacific J Trop Med 2013;16:325-32.
Vandana Srivastava, Arvind Singh Negi, Kumar JK, Gupta MM, Suman Khanuja PS. Plant-based anticancer molecules. A chemical and biological profile of some important leads. Bioorg Med Chem 2005;13:5892–908.
Boyd MR. The NCI in vitro anticancer drug discovery screen: concept, implementation, and operation. In: Anticancer Drug Development Guide: Preclinical Screening, Clinical Trials, and Approval. Ed. Teicher BA. Humana Press: Totowa, NJ; 1997. p. 23-42.
Ferreira PMP, Santos AG, Tininis A, Costa PM, Cavalheiro AJ, Bolzani VS, et al. Casearin X exhibits cytotoxic effects in leukemia cell striggered by apoptosis. Chem Biol Interact 2010;188:497–504.