DETERMINATION OF ANTIBACTERIAL, ANTIFUNGAL, BIOACTIVE CONSTITUENTS OF TRIPHALA BY FT-IR AND GC-MS ANALYSIS


Eugin Amala Varuvel, M. Jeyaraj

Abstract


Objectives: To characterize the number of phytoconstituents present in triphala using FT-IR and GC-MS.

Methods: Antibacterial activity was measured by disc diffusion method, antifungal activity were analyzed by poisoned food technique, organic analysis was done by FT-IR, phytocomponents were identified by GC-MS analysis.

Results: The major bioactive components were present in methanolic extracts, further screened by GC-MS analysis revealed the presence of 10 bioactive compounds. The results were presented that triphala contains richly 1,2,3-Benzenetriol, 2-Furancarboxaldehyde, 5-(hydroxymethyl)-, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Furfural, 2H-Pyran-2,6(3H)-dione, D-Allose, n-Hexadecanoic acid, DL-Proline, 5-oxo-, methyl ester, Undecanol-5, 9-Phenanthrenol

Conclusion: Present findings indicated promising antimicrobial and phytocomponents are present and having remarkable number of qualities.

Keywords: Triphala, Antimicrobial activity, FT-IR and GC-MS.


References


Kulkani P.H. Clinical Assessment of the effect of Sookshma Triphala in Lipoma. J Ayurvedic Res Papers 1995;66-71.

Nadkarni A.K. Indian Materia Medica, Popular Press Ltd, Mumbai, 1976;3rd edn, 1308-15.

Singh P.K. Mycotoxin elaboration in Triphala and its constituents. J Indian Phytopathol 2003;56:380-3.

Reddy TC, Aparoy.P, et al. Kinetics and docking studies of a COX-2 inhibitor isolated from Terminalia bellerica fruits. J Protein Pept Lett 2010;17:1251-7.

Gulati R., Agarwal S. Indian J Exp Biol 1995;33:261-8.

Juss S.S. Triphala-the wonder drug. Indian Med Gaz 1997;131:194-6.

Naik GH, Priyadarshini KI, Hari Mohan. Free radical scavenging reactions and phytochemical analysis of Triphala an ayurvedic formulation. J Curr Sci 2006 90(8):1100-05.

Thakur CP et al. The Ayurvedic Medicines Haritaki, amla and Bahira reduce cholesterol-induced atherosclerosis in rabbits. Int J Cardiol 1988;21:167-75.

Eugin Amala.V, Jeyaraj.M., Biometric, functional group analysis, antibacterial and antifungal activity of Terminalia bellirica Roxb and Phyllanthus emblica L. against some Pathogenic Microorganism. Int J of Sci Res 2014;3(4):531-3.

EuginAmala.V, Jeyaraj.M, Phytochemical, antibacterial and functional group identification of medicinally useful plant Terminalia Chebula Retz., against Staphylococcus aureus, Psuedomonas aeruginosa and Klebsilella pnuemoniae. Indiam J of Ap Res 2014;4(1):23-5.

Agarwal et al. Antibacterial activity of hydro alcoholic extract of Terminalia chebula Retz, on different gram positive and gram negative bacteria. Int J Phar Biol Arch 2010;1(4):458-8.

Kokate C.K, Practical Pharmacognosy, Vallabh Prakashan, Mohammed A., Text Book of Pharmacognosy, CBS Publishers and Distributors 2001;218.16.

Bayer et al. Antibiotic susceptibility testing by standard single disc 1966;13-18.

Schmitz H. Food poisoned technique. J Industrial Engineering Chemical Analyst Education 361-3.

Ogunlesi, M., W. Okiei, E.A.S. Osibote and C. Muotoh. Antimicrobial activity of the essential oil and the fractional samples obtained from the leaves and seeds of Phyllanthus amarus (Euphorbiaceae). Res J Phytochem 2009;3:77-84.

Ganesh,S. and J.J. Vennila,. Phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis by GC-MS. Res J Phytochem 2011;5:60-5.



About this article

Title

DETERMINATION OF ANTIBACTERIAL, ANTIFUNGAL, BIOACTIVE CONSTITUENTS OF TRIPHALA BY FT-IR AND GC-MS ANALYSIS

Date

01-09-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 8, 2014 Page: 123-126

Online ISSN

0975-1491

Statistics

310 Views | 538 Downloads

Authors & Affiliations

Eugin Amala Varuvel
Bharathidasan University, Thichirappalli
India

M. Jeyaraj
India

 

Article Tools


Share

Refbacks

  • There are currently no refbacks.