MIMOSA PUDICA EXERTS NEUROPROTECTION AGAINST MPP+INDUCED NEUROTOXICITY IN SHSY5Y CELL LINES-AN IN VITRO MODEL OF ANTI-PARKINSONISM

Mahadevan M. V., Ramaswamy R. S., Banumathi V.

Abstract


Objective: Parkinson’s disease (PD) is one of the of a neurodegenerative disorder, It’s decreased the dopaminergic neurones, tyrosine hydroxylase (TH) and increased the α-synuclein protein level. This study was conducted to investigate the neuroprotective effect of Mimosa pudica have the abilities to improve TH and DAT proteins expression against MPP+ induced neurotoxicity, in in vitro model of Parkinson’s disease using SH-SY5Y human neuroblastoma cell lines.

Methods: Mimosa pudica were pre-treated with various concentration for cell viability assay. Vehicle alone or Mimosa pudica (300µg) for 24 h, and then were co-treated with 1000µM MPP+ for 15 min in the continued presence of vehicle or Mimosa pudica. After treatment, cells were collected for protein expression.

Results: Cell viability assay confers the inhibitory concentration cell death of Mimosa pudica. MPP+significantly down-regulated the protein expression of TH (p<0.01) and DAT (p<0.05). Mimosa pudica decreased the expression of α synuclein (p<0.01) in MPP+ intoxicated cell lines.

Conclusion: The present study showed that Mimosa pudica exerts neuroprotection by suppressing α synuclein and the dopaminergic neurdegeneration. Mimosa pudica may be due to quecertin which might be acted via the anti-oxidant mechanism. The above finding suggests that Mimosa pudica may act as a potential target in the management of PD.

 


Keywords


Parkinson’s disease, Mimosa pudica, Siddha medicine, SH-SY5Y, MPP+, α synuclein, Tyrosine hydroxylase, Dopamine Transporter

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DOI: http://dx.doi.org/10.22159/ijpps.2017v9i1.15728

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