EVALUATION OF ANTI-OBESITY ACTIVITY OF SOLASODINE IN HIGH FAT DIET-INDUCED OBESITY IN RAT

Authors

  • Satish Khaserao Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India
  • Rahul Somani Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India

DOI:

https://doi.org/10.22159/ijpps.2017v9i3.16025

Keywords:

Obesity, Metabolic syndrome, Solasodine, Solanum xanthocarpum, HF diet-induced obesity

Abstract

Objective: This study was planned to study the anti-obesity activities of solasodine on high fat (HF) diet-induced obese rats.

Methods: Wistar rats were divided into six groups. Control group (Group 1) received normal diet and 0.5 % CMC (5 ml/kg). HF control group (Group 2) received HF diet. Group 3 received orlistat (25 mg/kg body weight per oral). Group 4, 5 and 6 received 25, 50 and 100 mg/kg body weight solasodine respectively. Treatment was given for 6 w to the respective group along with HF diet. Body weight, food intake and abdomen circumference was measured every week for 6 w. On day 42, the serum biochemical parameters like blood glucose and insulin, serum leptin, total cholesterol and triglyceride were evaluated. Animals were sacrificed with overdose of diethyl ether. The liver and retroperitoneal adipose tissues were removed and weighed immediately.

Results: Treatment with solasodine at dose of 50 mg/kg and 100 mg/kg significantly (p<0.001) reduced body weight, abdomen circumference and retroperitoneal adipose tissue weight as compared to the HF diet control group. Solasodine also significantly reduced serum total cholesterol, triglyceride and glucose level as compared to HF diet control group (***p<0.001, **p<0.01, *p<0.05 when compared with normal control. ###p<0.001, #p<0.05 when compared with high fat control). In addition, it also inhibited the induction of fatty liver with accumulation of hepatic triglyceride.

Conclusion: Solasodine exhibited anti-obesity effect by showing a reduction in body weight, abdomen circumference, total cholesterol level, triglyceride level and glucose level in high-fat diet fed rats.

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References

Alberti KG, Zimmet P, Shaw J. The metabolic syndrome–a new worldwide definition. Lancet 2005;366:1059–62.

Alisi A, Manco M, Panera N, Nobili V. Association between type two diabetes and non-alcoholic fatty liver disease in youth. Ann Hepatol 2009;8(Suppl 1):S44-50.

Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey ML. Pharmacotherapy: A Pathophysiologic Approach. 6th Edn. The McGraw-Hill Companies, New York; 2005. p. 2662-70.

Palatty PL, Saldanha E. Pharmacotherapy for weight management. J Assoc Physicians India 2012;60:34-5.

Yun JW. Possible anti-obesity therapeutics from nature–a review. Phytochem 2010;71:1625-6.

Anitha M, Roselin ME, Monisha DM, Chella Karthik J. Prevalence of obesity and overweight among medicos in both male and female Students. Asian J Pharm Clin 2016;9:289-91.

Kaur G, Kulkarni SK. Antiobesity effect of a polyherbal formulation, OB-200G in female rats fed on cafeteria and atherogenic diets. Indian J Pharmacol 2000;32:294-8.

Kumar S, Alagwadi KR, Rao MR. Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats. Indian J Pharmacol 2012;43:163-6.

Thacker T. Slimming drug sibutramine banned in India; 2010. Available from: http://archive.indianexpress.com/ news/ slimming-drug-sibutramine-banned-in-india/710526/ [Last accessed on 01 Oct 2016].

Lim DW, Song Mi Kyung, Park Juyeon, Park SW, Kim NH, Gaire BP, et al. Anti-obesity effect of HT048, a herbal combination, in high fat diet-induced obese rats. Molecules 2012;17:14765-7.

Arcari DP, Bartchewsky W. Antiobesity effects of Yerba mate extract (Ilex paraguariensis) in high-fat diet–induced obese mice. Obesity 2009;17:2127-33.

Prats E, Monfar M, Castella J. Energy intake of rats fed a cafeteria diet. Physiol Behavior 1989;45:263-72.

Dhingra D, Jindal V, Sharma S. Evaluation of antiobesity activity of Tinospora cordifolia stems in rats. Int J Res Ayurveda Pharm 2011;2:306-11.

Hussain T, Gupta RK, Khan MS. Evaluation of the anti-hepatotoxic potential of Solanum xanthocarpum fruit extract against antitubercular drugs induced hepatopathy in experimental rodents. Asian Pac J Trop Biomed 2012;1:1-6.

Balachandran P, Govindaraja R. Cancer an ayurvedic perspective. Pharmacol Res 2005;51:19-30.

Rasheeduz Z, Parisa H. Quantification of solasodine in callus and suspension cultures of Solanum xanthocarpum Schrad and Wendl by RP-HPLC. Int J Pharm Phytochem Res 2015;7:206-14.

Kar DM, Maharana L, Dash GK. Studies on the hypoglycemic activity of Solanum xanthocarpum Schrad and Wendl fruit extract in rats. J Ethnopharmacol 2006;108:251-6.

Girish C, Koner BC. Hepatoprotective activity of six polyherbal formulations in paracetamol-induced liver toxicity in mice. Indian J Med Res 2009;129:569-78.

Parmar S, Gangwal A, Sheth N. Solanum xanthocarpum (Yellow-berried nightshade): a review. Der Pharm Lett 2010;2:373-83.

Rao MM, Kandale A, Sharma K, Singh U. Evaluation of phytochemical and standardisation parameters of Solanum xanthocarpum Schrad and Wendl. Int J Chem Anal Sci 2010;1:47-9.

Sharma T, Airao V, Panara N, Vaishnav D, Ranpariya V, Sheth N, et al. Solasodine protects rat brain against ischemia/reperfusion injury through its antioxidant activity. Eur J Pharmacol 2014;725:40-6.

Gawande A, Wankhade S, Shankhopal KV, Shinde GB. Isolation, purification and characterization of solasodine–A steroidal alkaloid from Solanum surattense berries. Indian Drugs 1991;28:149-50.

Chauhan K, Sheth N, Ranpariya V, Parmar S. Anticonvulsant activity of solasodine isolated from Solanum sisymbriifolium fruits in rodents. Pharm Biol 2011;49:194-9.

Athesh K, Divakar M, Brindha P. Anti-obesity potential of Cyperus rotundus. aqueous tuber extract in rats fed on high-fat cafeteria diet. Asian J Pharm Clin Res 2014;7:88-92.

Mahmoud AAA, Elshazly SM. Ursodeoxycholic acid ameliorates fructose-induced metabolic syndrome in rats. PLoS One 2014;9:e106993.

Considine R, Sinha M, Serum immunoreactive leptin concentrates in normal weight and obese human. N Engl J Med 1996;334:292-5.

Sampathkumar R, Balasubramanyam M, Rema M, Premanand C, Mohan V. A novel advanced glycation index and its association with diabetes and microangiopathy. Metab Clin Exp 2005;54:1002-7.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130–2.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70–7.

Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF. Homoeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985;28:412–9.

Gupta S, Kaushik M. Histomorphological and hypolipidaemic effects from the whole plant of Gymenma sylvestre in high cafeteria diet-induced obese rat model. J Pharm Biomed Sci 2010;2:1-9.

Castejon MG, Casado AR. Dietary phytochemicals and their potential effects on obesity: a review. Pharmacol Res 2011;2:1-18.

Alshawsh MA, Abdulla MA, Ismail S, Amin ZA. Hepatoprotective effects of Orthosiphon stamineus extract on thioacetamide-induced liver cirrhosis in rats. J Evidence-Based complimentary Altern Med 2011:1-6. http://dx.doi.org/10.1155/2011/ 103039

Han LK, Zheng YN, Yoshikawa M, Okuda H, Kimura Y. Antiobesity effects of chikusetsu saponins isolated from Panax japonicas rhizomes. BMC Complementary Altern Med 2005;6:5-9.

Yun SN, Moon SJ, Ko SK, Im Bo. Wild ginseng prevents the onset of high-fat diet induced hyperglycemia and obesity in ICR mice. Arch Pharm Res 2004;7:790-6.

Han LK, Zheng YN, Xu BJ, Kimura Y. Saponins from Platycodi radix ameliorate high fat diet-induced obesity in mice. J Nutr 2002;132:2241-5.

Yen TT, Allan JA, Pearson DV. Control of obesity in Avy/a mice by 5alpha-androstan-17-one. Experientia 1978;34:1542-3.

Published

01-03-2017

How to Cite

Khaserao, S., and R. Somani. “EVALUATION OF ANTI-OBESITY ACTIVITY OF SOLASODINE IN HIGH FAT DIET-INDUCED OBESITY IN RAT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 3, Mar. 2017, pp. 23-29, doi:10.22159/ijpps.2017v9i3.16025.

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