EXTRACTION AND CHARACTERIZATION OF IRAQI ARTEMISIA DRACUNCULUS DRIED AERIAL PARTS EXTRACT THOUGH HPLC AND GC-MS ANALYSIS WITH EVALUATION OF ITS ANTITUMOR ACTIVITY AGAINST 7, 12-DIMETHYLBENZE (a) ANTHRACENE INDUCED SKIN CANCER IN MICE

Authors

  • Nabaa Mohammed Ibrahem College of Pharmacy, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.22159/ijpps.2017v9i5.16875

Keywords:

Artemisia dracunculus L, Quercetin, Kampferol, Rutin, Skin cancer

Abstract

Objective: This study focused on the extraction of bioactive constituents in Iraqi cultivated Artemisia dracunculus L dried aerial parts and phytochemical screening with qualitative estimation of three important flavonols (kampferol, quercetin and rutin). The study also included evaluating the antitumor effects (skin cancer) of methanolic extract for this plant.

Methods: Extraction of bioactive constituents was carried out according to standard protocols using 85% methanol. The phytochemical screening has been done using chemical tests and GC/MS with qualitative estimation of three flavonols (kampferol, quercetin and rutin) utilized preparative high-performance liquid chromatography (PHPLC). Skin cancer in mice was induced by using 7, 12-dimethylbenze (a) anthracene to evaluate the antitumor effect of methanolic extract of Artemisia dracunculus when administered orally via intragastric feeding tube. Body weight, tumor volume, tumor burden, percent of tumor incidence and histopathological examination were measured in all tested animals.

Results: Phytochemical screening revealed the presence of glycosides, volatile oils, alkaloids, tannin, terpenoids, phenolic compounds and flavonoids. The preparative high-performance liquid chromatography (PHPLC) analysis indicated that rutin had the highest concentration followed by quercetin while kampferol had the lowest concentration. The methanolic extract of the Artemisia dracunculus significantly reduced the percent of tumor incidence, tumor volume and tumor burden of skin cancer in mice (p<0.05).

Conclusion: Dried aerial parts of Iraqi Artemisia dracunculus contain many bioactive constituents including three flavonoid compounds (quercetin, kampferol and rutin) that may be responsible for chemo preventive effect exerting by methanolic extract of Artemisia dracunculus against 7, 12-dimethylbenzeanthracine-induced skin cancer in mice.

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References

Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Res 2000;17:215-34.

Obolskiy D, Pischel I, Feistel B, Glotov N, Heinrich M. Artemisia dracunculus L. (tarragon): a critical review of its traditional 2 use, chemical composition, pharmacology, and safety. J Agric Food Chem 2011;59:11367-84.

Aglarova AM, Zilfikarov IN, Severtseva OV. Biological characteristics and useful properties of tarragon (Artemisia dracunculus L.). Pharm Chem J 2008;42:81-6.

Uhl SR, Strauss S. Handbook of Spices, Seasonings and Flavorings. Technomic Publishing: Lancaster PA; 2000. p. 170-1.

Mamedov N, Grdner Z, Craker LE. Medicinal plants used in Central Asia for the treatment of selected skin conditions. J Herbs Spices Med Plants 2004;11:191–222.

Alakbarov FU. Medicinal plants used in medieval Azerbaijan phytotherapy. J Herbal Pharmacother 2001;3:35–49.

Ribnicky DM, Kuhn P, Poulev A, Roopchand DE., Oren A, Cefalu WT, et al. Artemisia dracunculus L. polyphenols complexed to soy protein show enhanced bioavailability and hypoglycemic activity in C57BL/6 mice. Nutrition 2014;30 Suppl 7-8:54-510.

Kirk-Ballard H, Wang ZQ, Acharya P, Zhang XH, YU Yongmei, Klory Gail, et al. An Extract of Artemisia dracunculus L. Inhibits ubiquitin-proteasome activity and preserves skeletal muscle mass in a murine model of diabetes. PLoS One 2013;8:e57112.

Firestone GL, Sundar SN. Anticancer activities of artemisinin and its bioactive derivatives. Expert Rev Mol Med 2009;30:11-32.

Joshi UJ, Gadge AS, D`mello P, Sinha R, Srivastava S, Govil G. Anti-inflammatory, the antioxidant and anticancer activity of quercetin and its analogues. Int J Res Pharm Biomed Sci 2011;2:1757-66.

Mohana DC, Satish S, Raveesha KA. Antibacterial evaluation of some plant extracts against some human pathogenic bacteria. Adv Biol Res 2008;2:49-55.

Abdul-Jalil Thg Z. Phytochemical and antimicrobial study of some flavonoids present in the fruits of two Ammi L. species wildly grown in Iraq. M. Sc thesis. Baghdad University; 2009. p. 42-3.

Kadhim EJ. Phytochemical investigation and testing the effect of Iraqi Echinopsheterophyllus family Compositae on wound healing. PH. D thesis. Bagdad University; 2013. p. 76-7.

Maraie NK, Abdul-Jalil Thg Z, Alhamdany AT, Janabi HA. Phytochemical study of the Iraqi beta vulgeris leaves and its clinical application for the treatment of different dermatological diseases. World J Pharm Pharm Sci 2014;3:5-19.

Khadim EJ, Abdul-rassol AA, Awad ZJ. Phytochemical investigation of alkaloids in the Iraqi Echiropsheterophyllus (Compositae). Iraqi J Pharm Sci 2014;23:26-34.

Bargah RK. Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma Gaertu. J Pharmacogn Phytochem 2015;4:7-9.

Proestos C, Komaitis M. Analysis of naturally occurring phenolic compounds in aromatic plants by RP-HPLC coupled to diode array detector (DAD) and GC-MS after silylation. Foods 2013;2:90-9.

Obistioiu D, Cristina RT, Schmerold I, Chizzola R, Stolze K, Nichita I, et al. Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chem Central J 2014;8:2-11.

Spencer JPE, Vanzour D, Rendeiro C. Flavonoids and cognition: the molecular mechanisms underlying their behavioral effects. Arch Biochem Biophys 2009;492:1-9.

Hussein SA. Phytochemical study of some medicinal compounds presents in Hedera helix L. plant cultivated in Iraq. M Sc. thesis Baghdad University; 2014:20.

Javier ER, Ricardo Z, Beatriz S, Mario JS. Antioxidant properties and hyphenated HPLC-PDA-MS profiling of chilean fruits (Mangifera indica L. Cv. piqueño). Molecules 2014;19:438-58.

Chaudary G. Inhibition of dimethylbenze (a) anthracene (dmba) or croton oil-induced skin tumorigenesis in Swiss albino mice by Aloe vera treatment. Int J Biol Med Res 2011;2:671-8.

Zime-Diawara H, Ganfon H, Gbaguidi F, Yemoa A, Bero J, Jansen O, et al. The antimalarial action of aqueous and hydroalcoholic extracts of Artemisia annua L. cultivated in Benin: in vitro and in vivo studies. J Chem Pharm Res 2015;7:817-23.

Azuine MA, Bhide SV. Chemopreventive effect of turmeric against stomach and skin tumors induced by chemical carcinogens in swiss mice. Nutr Cancer 1992;17:77.

Rodzi R, Cheah YL, Ooi KK, Othman F, Mohtarrudin N, Tohid SF, et al. Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis. Afr J Pharm Pharmacol 2013;7:2484-98.

Kshirsagar AD, Mohite R, Agrawal AS, Suralkar UR. Hepatoprotective medicinal plants of Ayurveda-a review. Asian J Pharm Clin Res 2011;4:1­8.

Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 2008;69:1732–8.

Eisenman SW, Poulev A, Struwe L, Raskin I, Ribnicky DM. Qualitative variation of anti-diabetic compounds in different tarragon (Artemisia dracunculus L.) cytotypes. Fitoterapia 2011;82:1062-74.

Seyed AE, Nasser VM, Remisa V, Mohammad BO. The anticancer activity of five species of artemisia on hep2 and hepG2 cell Lines. Pharmacologyonline 2009;3:327-39.

Alabdallat Nessrin G. In vitro antisickling activity of Artemisia Herba-Alba asso (CHIH) methanolic extract on sickle cell disease. Asian J Pharm Clin Res 2016;9 Suppl 1:109-12.

Dixit Savita. Anticancer effect of rutin isolated from the methanolic extract of Triticum aestivum straw in mice. Med Sci 2014;2:153-60.

Ke. Yao, Hanyong C, Kangdong L, Alyssa L, Ge Y, Yi Z, et al. Kaempferol targets RSK2 and MSK1 to suppress UV radiation-induced skin cancer. Cancer Prev Res 2014;7:958-67.

Ali H, Dixit Savita. Quercetin attenuates the development of 7, 12-dimethyl benz (a) anthracene (DMBA) and croton oil-induced skin cancer in mice. J Biomed Res 2015;29:139-44.

Published

01-05-2017

How to Cite

Ibrahem, N. M. “EXTRACTION AND CHARACTERIZATION OF IRAQI ARTEMISIA DRACUNCULUS DRIED AERIAL PARTS EXTRACT THOUGH HPLC AND GC-MS ANALYSIS WITH EVALUATION OF ITS ANTITUMOR ACTIVITY AGAINST 7, 12-DIMETHYLBENZE (a) ANTHRACENE INDUCED SKIN CANCER IN MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 5, May 2017, pp. 34-42, doi:10.22159/ijpps.2017v9i5.16875.

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Original Article(s)