BROWN SEAWEEDS AMELIORATE RENAL ALTERATIONS IN MICE TREATED WITH THE CARCINOGEN AZOXYMETHANE
Keywords:
Azoxymethane, Nephrotoxicity, Seaweeds, Oxidative stressAbstract
Objectives: The current study was conducted to evaluate the possible protective effects of the brown seaweeds, Turbinaria ornata and Padina pavonia, against azoxymethane (AOM)-induced nephrotoxicity in mice.
Methods: The experimental mice were allocated randomly into 4 groups as follows; Group I (Normal): mice received two consecutive doses of saline, Group II (AOM): mice received intraperitoneal injections of 10 mg/kg AOM once a week for two consecutive weeks, Group III (AOM + T. ornata): mice received AOM once a week for two consecutive weeks + 100 mg/kg T. ornata extract, and Group IV (AOM + P. pavonia): mice received AOM once a week for two consecutive weeks + 100 mg/kg P. pavonia extract. Both extracts were supplemented orally for 3 weeks starting at the end of 10th week.
Results: AOM administration to mice induced renal damage evidenced by the histological alterations, including blood vessel dilatation, degenerated tubules, necrosis, inflammatory cell infiltrations, mildly congested blood vessels, degenerated glomeruli, thickened blood vessel wall and other histologic manifestations. In addition, AOM-administered mice showed significantly increased serum creatinine, urea and uric acid as well as elevated renal lipid peroxidation and nitric oxide levels. Consequently, renal glutathione content and superoxide dismutase and glutathione peroxidase activities were significantly declined. Supplementation of either T. ornata or P. pavonia markedly alleviated the AOM-induced alterations.
Conclusion: The current findings provide the first evidence that T. ornata and P. pavonia could protect mice against AOM-induced renal damage via abolishment of oxidative stress and potentiation of the antioxidant defense system.
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