SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DOXORUBICIN HYDROCHLORIDE

Authors

  • Nayan Pradhan Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Hiyashree Rajkhowa Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Hemant Giri Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Bhupendra Shrestha Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India

Keywords:

Moxifloxacin hydrochloride, Doxorubicin hydrochloride, Simultaneous estimation, Method development, Validation

Abstract

Objective: To develop a simple, accurate, and precise spectrophotometric method for the simultaneous estimation of moxifloxacin hydrochloride (MOX) and doxorubicin hydrochloride (DXR).

Methods: MOX and DXR solution were simultaneously determined in 0.1M HCl at their respective λmax. The absorbance λmax of MOX and DXR was 295 nm and 480 nm, respectively. The developed method was validated according to ICH guidelines for parameters like linearity, accuracy, precision, ruggedness and robustness.

Results: Molar absorptivities of MOX and DXR were found to be more in 0.1M HCl with compared to water, methanol and 0.1M NaOH. Linearity was obtained over the range 0.5–20.0 μg/ml and 1.5-50 μg/ml with a lower limit of quantitation of 0.25 μg/ml and 1.5 μg/ml for MOX and DXR, respectively. For each level of samples, inter-and intra-day precision ( % RSD) was<1.3 % and<1.4 % for MOX and<2.5 and 2.4 % for DXR, respectively. The mean recovery of MOX and DXR was in the range 96.21 %-98.77 % and 97.13 %-99.64 %, respectively.

Conclusion: The method developed was validated as per ICH guidelines for parameters like linearity, accuracy, method precision, robustness and ruggedness. The results obtained were well within the acceptable criteria. The method can be used for routine analysis of MOX and DXR.

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Published

01-11-2015

How to Cite

Pradhan, N., H. Rajkhowa, H. Giri, and B. Shrestha. “SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DOXORUBICIN HYDROCHLORIDE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 21-26, https://journals.innovareacademics.in/index.php/ijpps/article/view/4387.

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