CYTOTOXIC EFFECT OF HELIOTROPIUM INDICUM EXTRACTS ON HELA CELL LINE
Keywords:
Heliotropium indicum, Hela cell lines, MTT assay, Stem and leaf alcohol extractAbstract
Objective: Heliotropium indicum (H. indicum) has been used widely for centuries on warts and to treat inflammations and tumors. H. indicum Linn (Family Boraginaceae) is a medicinal plant. It is known commonly as Cock's combâ€. H. indicum Linn has various medicinal uses in the treatment of disease conditions such as Abdominal pains, Amenorrhoea, Dysmenorrhoea, Skin rashes, Wounds, Hypertension, Ocular infections, Convulsion, Dizziness. To provide scientific validation to the traditional use of plants in vitro cytotoxic effect was evaluated on HeLa cell lines.
Methods: The crude extracts of stem and leaf of Heliotropium indicum were prepared using soxhlet apparatus using solvents of increasing polarity. The study was performed using methanolic extracts against human cancer cell line (HeLa) using MTT assay to analyze the cell growth inhibition.
Results: The results showed that the methanolic extracts of stem and leaf of Heliotropium indicum possessed a good amount of anticancer activity and IC50 for both the extracts found to be 200µg/ml. Whereas stem extracts exhibited excellent activity up to 64.5% at 200 µg/ml and followed by leaf extract up to 49.67% at 200 µg/ml. Obtained results supports the traditional claim of the plant.
Conclusion: The methanolic extracts of leaf and stem of H. indicum exhibits cytotoxic activity. Hence, Obtained results support the traditional claim of the plant.
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References
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