ETHYL ACETATE FRACTION OF GARCINIA MANGOSTANA-LINN PERICARP EXTRACT: ANTI-C.ALBICANS AND EPITHELIAL CYTOTOXICITY

  • Febrina Rahmayanti Rahmayanti Faculty of Dentistry Universitas Indonesia
  • Dewi Fatma Suniarti Universitas Indonesia
  • Zainal Alim Mas'ud Institute of Agricultural Bogor
  • Boy Muchlis Bachtiar Universitas Indonesia
  • Yuniardini Septorini Wimardhani Universitas Indonesia
  • Gus Permana Subita Universitas Indonesia

Abstract

Candida spesies are commensal agent in oral, skin, and GIT environment, but when there is underlying predisposing condition in host, it causing candida infections. The increasing of antifungal resistance complicate patient management, therefore it is necessary to identify potential antifungal agents. Garcinia mangostana-Linn (GML) is a famous tropical fruits in Indonesia and other south-east Asia countries. This study was conducted to examine the antifungal activity of chromatographic coloumn ethyl acetate fraction of GML pericarp against C.albicans. GML was extracted with ethanol and fractionated with ethyl acetate. C.albicans ATCC 10231 was used in this study. Antifungal activities were expressed as viability of Candida albicans growth identification with MTT assay. There is no previous research that analyzes the antifungal effect of GML based on ethyl acetate fraction from the chromatographic fractionation technique, especially as anti-C.albicans. In this study, we also conducted an analysis of the cytotoxicity effect of ethyl acetate fraction on HaCaT cell line. The result of our study indicate that ethyl acetate fraction of GML pericarp extract have reduced the viability of C.albicans. There is a tendency of decrease in viability in line with the increase of concentration of fraction. As anti-C.albicans ethyl acetate fraction of GML pericarp extract has no cytotoxicity to HaCaT cell line. This result showed the ability of ethyl acetate fraction of GML extract changes the viability of C. albicans, but not toxic to HaCaT cell line.  It may consider ethyl acetate fraction of GML pericarp extract as potent anti-C.albicans and promising adjuncts in oral health product.

Author Biographies

Febrina Rahmayanti Rahmayanti, Faculty of Dentistry Universitas Indonesia
Oral Medicine Department
Dewi Fatma Suniarti, Universitas Indonesia
Oral Biology Department
Zainal Alim Mas'ud, Institute of Agricultural Bogor
Department of Chemical-Integrated Laboratory
Boy Muchlis Bachtiar, Universitas Indonesia
Oral Biology Department
Yuniardini Septorini Wimardhani, Universitas Indonesia
Oral Medicine Department
Gus Permana Subita, Universitas Indonesia
Oral Medicine Department

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How to Cite
Rahmayanti, F. R., D. F. Suniarti, Z. A. Mas’ud, B. M. Bachtiar, Y. S. Wimardhani, and G. P. Subita. “ETHYL ACETATE FRACTION OF GARCINIA MANGOSTANA-LINN PERICARP EXTRACT: ANTI-C.ALBICANS AND EPITHELIAL CYTOTOXICITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 357-60, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10088.
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Original Article(s)