LIQUORICE BEVERAGE EFFECT ON THE PHARMACOKINETIC PARAMETERS OF ATORVASTATIN, SIMVASTATIN, AND LOVASTATIN BY LIQUID CHROMATOGRAPHY-MASS SPECTROSCOPY/MASS SPECTROSCOPY

Authors

  • Wael A Abu dayyih University of Petra
  • Eyad M Mallah University of Petra
  • Israa H Al-ani Al-Ahliyya Amman University
  • Tawfiq A Arafat

Abstract

ABSTRACT
Objective: The objective of this study is to examine the effects of pre-consumption of freshly prepared liquorice beverage (4 ml/kg) on the
pharmacokinetic (PK) parameters of (80 mg/kg) oral dose of atorvastatin, simvastatin, and lovastatin in healthy rats plasma.
Methods: A simple, rapid, and applicable analytical method was developed for the determination of each statin in rats' plasma. This method uses
liquid chromatography-mass spectroscopy/mass spectroscopy. The mobile phase composed of methanol and formic acid in water and glimepiride as
an internal standard. 108 rats were used in this study. Liquorice juice was given, and then each of the statins was given to test groups and liquorice
only to the control groups, and then plasma samples were withdrawn on specific time schedule then PK analysis was performed.
Results: The analytical method showed acceptable linearity, recovery, precision, and accuracy. Administration of liquorice resulted in a significant
increase in maximum concentration in plasma (C
) of the three statins, also the area under plasma level-time curves (area under curve) was increased
significantly. Moreover, the bioavailability of the drugs. On the other hand, the elimination of the three drugs showed no great changes, which suggests
an interaction between liquorice and the transporting system of statins on the gut and biliary wall.
max
Conclusion: Consumption of liquorice results in increase bioavailability of atorvastatin, simvastatin, and lovastatin.
Keywords: Liquorice, Atorvastatin, Liquid chromatography-mass spectroscopy/mass spectroscopy, Simvastatin, Lovastatin, Pharmacokinetic
parameters.

Author Biographies

Wael A Abu dayyih, University of Petra

associated professor in Department of Pharmaceutical Medical Chemistry and Pharmacognosy

Eyad M Mallah, University of Petra

Associated professor in Department of Pharmaceutical Medical Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences.

Israa H Al-ani, Al-Ahliyya Amman University

Assist. professor of pharmaceutics in department of pharmaceutical sciences, faculty of pharmacy and medical sciences.

References

REFERENCES

Alshehri MM. A validated capillary electrophoresis method for

simultaneous determination of ezetimibe and atorvastatin in

pharmaceutical formulations. Saudi Pharm J 2012;20(2):143-8.

Stancu C, Sima A. Statins: Mechanism of action and effects. J Cell Mol

Med 2001;5(4):378-87.

Goldberg A, Hopkins P, Toth P, Ballantyne C, Rader D, Robinson J, et al.

Familial hypercholesterolemia: Screening, diagnosis and management

of pediatric and adult patients: Clinical guidance from the National

Lipid Association Expert Panel on Familial Hypercholesterolemia.

J Clin Lipidol 2011;5(3):1-8.

Khan FN, Dehghan MH. Enhanced bioavailability of atorvastatin

calcium from stabilized gastric resident formulation. AAPS

PharmSciTech 2011;12(4):1077-86.

Qinna NA, Kamona BS, Alhussainy TM, Taha H, Badwan AA,

Matalka KZ. Effects of prickly pear dried leaves, artichoke leaves,

turmeric and garlic extracts, and their combinations on preventing

dyslipidemia in rats. ISRN Pharmacol 2012;2012:167979.

Braamskamp M, Wijburg F, Wiegman A. Drug therapy of

hypercholesterolaemia in children and adolescent. Drugs

;72(6):759-72.

Whirl-Carrillo M, McDonagh EM, Hebert JM, Gong L, Sangkuhl K,

Thorn CF, et al. Pharmacogenomics knowledge for personalized

medicine. Clin Pharmacol Ther 2012;92(4):414-7.

Lennernäs H. Clinical pharmacokinetics of atorvastatin. Clin

Pharmacokinet 2003;42(13):1141-60.

Mauro VF. Clinical pharmacokinetics and practical applications of

simvastatin. Clin Pharmacokinet 1993;24(3):195-202.

Kantola T, Kivistö KT, Neuvonen PJ. Grapefruit juice greatly increases

serum concentrations of lovastatin and lovastatin acid. Clin Pharmacol

Ther 1998;63(4):397-402.

Genser D. Food and drug interaction: Consequences for the nutrition/

health status. Ann Nutr Metab 2008;52 Suppl 1:29-32.

Tamimi L, Dayyih WA, Qinna N, Mallah E, Arafat T. Pioglitazone

levels and its pharmacokinetic application in presence of sucralose in

animals plasma, by HPLC method. Pharm Anal Acta 2014;5(9):318-25.

Le Goff-Klein N, Koffel JC, Jung L, Ubeaud G. In vitro inhibition of

simvastatin metabolism, a HMG-CoA reductase inhibitor in human and

rat liver by bergamottin, a component of grapefruit juice. Eur J Pharm

Sci 2003;18(1):31-5.

Tbeekh HT, Dayyih WA, Mallah E, Qinna N, Awad RM, Arafat TA.

Pomegranate juice effects on pharmacokinetic parameters of

metronidazole by using HPLC-MS. World J Pharm Pharm Sci

;3(7):150-4.

Siracusa L, Saija A, Cristani M, Cimino F, D’Arrigo M, Trombetta D,

et al. Phytocomplexes from liquorice (Glycyrrhiza glabra L.)

leaves – Chemical characterization and evaluation of their

antioxidant, anti-genotoxic and anti-inflammatory activity. Fitoterapia

;82(4):546-56.

Obolentseva GV, Litvinenko VI, Ammosov AS, Popova TP,

Sampiev AM. Pharmacological and therapeutic properties of licorice

preparations (a review). Pharm Chem J 1999;33(24):31-7.

Fiore C, Eisenhut M, Ragazzi E, Zanchin G, Armanini D. A history

of the therapeutic use of liquorice in Europe. J Ethnopharmacol

;99(3):317-24.

Al-Deeb ID, Arafat TA, Irshaid YM. The effect of licorice drink on

the systemic exposure of verapamil in rabbits. Drug Metab Lett

;4(3):173-9.

Won CS, Oberlies NH, Paine MF. Mechanisms underlying food-

drug interactions: Inhibition of intestinal metabolism and transport.

Pharmacol Ther 2012;136(2):186-201.

Neuvonen PJ, Niemi M, Backman JT. Drug interactions with lipidlowering

drugs: Mechanisms and clinical

relevance. Clin

Pharmacol

Ther

;80(6):565-81.

Dayyih WA, Al-Fayez A, Tamimi L, Mallah E, Arafat T. Simultaneous

determination of Atorvastatin, Glimepiride and Amlodipine in solution

and plasma matrix using HPLC/UV method. J Chem Pharm Res

;6(11):515-28.

Fukazawa I, Uchida N, Uchida E, Yasuhara H. Effects of grapefruit

juice on pharmacokinetics of atorvastatin and Simvastatin in Japanese.

Br J Clin Pharmacol 2004;57(4):448-5.

Code of Federal Regulation, Title 21, vol. 3. Available from: http:

www.accessdata.fda.gov. [Last accessed on 2015 Apr].

United State Pharmacopeia. Validation of compendial methods. US

Pharmacopial Convention. Ch. 1225. Rockville, MD: USP; 2010. p. 29.

Paine M, Khalighi M, Fisher J, Shen D, Kunze K, Mars C, et al.

Characterization of interintestinal and intraintestinal variations

in human CYP3A-dependent metabolism. J Pharmacol ExpTher

;283(3):1552-62.

Paine S, Parker A, Gardiner P, Webborn P, Riley R. Prediction of the

pharmacokinetics of atorvastatin, cerivastatin, and indomethacin using

kinetic models applied to isolated rat hepatocytes. Drug Metab Dispos

;36(7):1365-74.

Paine SW, Parker AJ, Gardiner P, Webborn PJ, Riley RJ. Prediction

of the pharmacokinetics of atorvastatin, cerivastatin, and indomethacin

using kinetic models applied to isolated rat hepatocytes. Drug Metab

Dispos 2008;36(7):1365-74.

Chouksey R, Pandey H, Jain AK, Soni H, Saraogi GK. Preparation

and evaluation of the self-emulsifying drug delivery system

containing atorvastatin HMG-COA inhibitors. Int J Pharm Pharm Sci

;3(3):147-52.

Kent UM, Aviram M, Rosenblat M, Hollenberg PF. The licorice root

derived isoflavan glabridin inhibits the activities of human cytochrome

P450S 3A4, 2B6, and 2C9. Drug Metab Dispos 2002;30(6):709-15.

Kuhn MA. Herbal remedies: Drug-herb interactions. Crit Care Nurse

;22(2):22-8.

Watanabe T, Kusuhara H, Maeda K, Kanamaru H, Saito Y, Hu Z, et al.

Investigation of the rate-determining process in the hepatic elimination

of HMG-CoA reductase inhibitors in rats and humans. Drug Metab

Dispos 2010;38(2):215-22.

Idkaidek M, Najib N, Arafat T, Al-Ghazawi A. Population

Pharmacokinetics of Atorvastatin, simvastatin and Pravastatin after oral

administration in human. Saudi Pharm J 2008;16(1):82-4.

Omar HR, Komarova I, El-Ghonemi M, Fathy A, Rashad R,

Abdelmalak HD, et al. Licorice abuse: Time to send a warning message.

Ther Adv Endocrinol Metab 2012;3(4):125-38.

Lilja JJ, Neuvonen M, Neuvonen PJ. Effects of regular consumption

of grapefruit juice on the pharmacokinetics of simvastatin. Br J Clin

Pharmacol 2004;58(1):56-60.

Wang X, Zhang H, Chen L, Shan L, Fan G, Gao X. Liquorice, a unique

guide drug†of traditional Chinese medicine: A review of its role in

drug interactions. J Ethnopharmacol 2013;150(3):781-90.

Hou YC, Lin SP, Chao PD. Liquorice reduced cyclosporine

bioavailability by activating P-glycoprotein and CYP 3A. Food Chem

;135(4):2307-12.

Tachjian A, Maria V, Jahangir A. Use of herbal products and potential

interactions in patients with cardiovascular diseases. J Am Coll Cardiol

;55(6):515-25.

Nowack R. Review article: Cytochrome P450 enzyme, and transport

protein mediated herb-drug interactions in renal transplant patients:

Grapefruit juice, St John’s Wort - and beyond! Nephrology (Carlton)

;13(4):337-47.

Gunturu S. Drug–nutrient interactions. In: Pitchumoni CS,

Dharmarajan T, editors. Geriatric Gastroenterology. New York:

Springer; 2012. p. 89-98.

Izzo AA. Interactions between herbs and conventional drugs: Overview

of the clinical data. Med Princ Pract 2012;21(5):404-28.

Published

2016-03-01

How to Cite

Abu dayyih, W. A., E. M. Mallah, I. H. Al-ani, and T. A Arafat. “LIQUORICE BEVERAGE EFFECT ON THE PHARMACOKINETIC PARAMETERS OF ATORVASTATIN, SIMVASTATIN, AND LOVASTATIN BY LIQUID CHROMATOGRAPHY-MASS SPECTROSCOPY/MASS SPECTROSCOPY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, Mar. 2016, pp. 174-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10249.

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