IN VITRO HYPOGLYCAEMIC EVALUATION OF SEVEN CULINARY PLANTS OF NORTH EAST INDIA AGAINST TYPE 2 DIABETES

Authors

  • Jayashree Dutta
  • Kalita Mc

Abstract

ABSTRACT
Objective: The goal of the present study is to provide an in vitro evidence for potential inhibition of the α-amylase and α-glucosidase enzyme by seven
culinary plants of North east India followed by their phytochemical screening.
Methods: The different parts (leaves, seeds, bark, and fruit) of the selected plants were chosen for the study. Collected plant parts were a shade dried,
powdered, and successively extracted using petroleum ether, acetone, and methanol. The obtained extracts were quantitatively assayed for in vitro
α-amylase and α-glucosidase enzyme inhibitory activity. The collected plant materials were screened qualitatively for detection of several bioactive
compounds.
Results: In our study, we found that among all the screened culinary plants the highest α-amylase inhibitory activity was demonstrated by Dillenia
indica Linn. fruit methanol extract with a minimum IC
value of 02.45 ± 0.305 mg/ml, and the highest α-glucosidase inhibitory activity was
demonstrated by D. indica leaves methanol extract with a very minimal IC
50
value of 01.78 ± 0.331 mg/ml compared to the standard drug acarbose
IC
50
- 05.43 ± 0.280 mg/ml for α-amylase and IC
50
-

50
03.06 ± 0.072 mg/ml for α-glucosidase, respectively. Phytochemical screening reveals the presence
of several bioactive groups such as carbohydrate, protein, saponin, tannin, flavonoid, alkaloid, and terpenoids in all studied plants.
Conclusion: The study concludes that selected culinary plants of North East India are capable of inhibiting carbohydrate metabolizing enzyme
α-amylase and α-glucosidase, and the presence of bioactive compounds in these plants add on the potentiality of these plants to reduce the postprandial
hyperglycemia.
Keywords: Diabetes mellitus, Post-prandial hyperglycemia, α-amylase, α-glucosidase, Culinary plants.

References

REFERENCES

Kumar S, Kumar V, Prakash OM. Antidiabetic and hypolipidemic

activities of Kigelia pinnata flowers extract in streptozotocin induced

diabetic rats. Asian Pac J Trop Biomed 2012;2(7):543-6.

Jaiswal N, Srivastava SP, Bhatia V, Mishra A, Sonkar AK,

Narender T, et al. Inhibition of alpha glucosidase by Acacia nilotica

prevents hyperglycemia along with improvement of diabetic

complication via aldose reductase inhibitors. J Diabetes Metab

;6:2-7.

Sales PM, Souza PM, Simeoni LA, Silveira D. a-Amylase inhibitors:

A review of raw material and isolated compounds from plant source.

J Pharm Pharm Sci 2012;15(1):141-83.

Nickavar B, Abolhasani L, Izadpanah H. Alpha amylase inhibitory

activities of six Salvia species. Iran J Pharm Res 2008;7:297-303.

Adefegha SA, Oboh G. In vitro inhibition activity of polyphenol-rich

extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds

against carbohydrate hydrolyzing enzymes linked to Type 2 diabetes

and Fe

-induced lipid peroxidation in rat pancreas. Asian Pac J Trop

Biomed 2012;2(10):774-81.

Narkhede MB. Evaluation of alpha amylase inhibitory potential of four

traditional culinary leaves. Asian J Pharm Clin Res 2012;5:75-6.

Jia W, Gao W, Tang L. Antidiabetic herbal drugs officially approved in

China. Phytother Res 2003;17(10):1127-34.

Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabatic

potential. J Ethnopharmacol 2002;81:81-100.

Shukia R, Sharma SB, Puri D, Prabhu KM, Murthy PS. Medicinal

plants for treatment of diabetes mellitus. Indian J Clin Biochem

;15 Suppl 1:169-77.

Dutta J, Kalita MC. Ethno anti diabetic plants used by a few tribes of

rural Kamrup District, Assam. Int J Pharm Sci Res 2013;4:3663-9.

Khan MH, Yadava PS. Antidiabetic plants used in Thoubal district of

Manipur, North East India. Indian J Tradit Knowl 2010;9:510-4.

Bernfield P. Amylases alpha and beta. In: Methods in Enzymology.

Vol. 1. New York: Academic Press; 1955. p. 149-58.

Artanti N, Firmansyah T, Darmawan A. Bioactivities evaluation of

Indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) leaves

extracts. J Appl Pharm Sci 2012;2:24-7.

Harborne JB. Phytochemical methods. A Guide to Modern Techniques

of Plant Analysis. 1

ed. London: Chapman and Hall; 1998. p. 182-90.

Yadav RN, Agarwala M. Phytochemical analysis of some medicinal

st

plants. J Phytol 2011;1 Suppl 3:813-37.

Trease GE, Evans WC. Pharmacognosy. 11

ed. London: Bailliere

Tindall; 1998. p. 45-50.

th

Suganya G, Sampath Kumar P, Dheeba B, Sivakumar R. In vitro

antidiabetic, antioxidant and anti-inflammatory activity of Clitoria

ternatea. Int J Pharm Pharm Sci 2014;6 Suppl 7:342-7.

Kumar DB, Mitra A, Manjunatha M. A comparative study of alpha

amylase inhibitory activities of common antidiabetic plants of

Kharagpur 1 block. Int J Green Pharm 2010;4:115-21.

Mohamed Sham Shihabudeen H, Hansi Priscilla D,

Thirumurugan K. Cinnamon extract inhibits a-glucosidase activity and

dampens postprandial glucose excursion in diabetic rats. Nutr Metab

(Lond) 2011;8(1):46.

Kavimani S, Saminathan K, Kumar S. In vitro antidiabetic activity

of dolichandrone atrovirens – An Indian medicinal plant. Int J

Pharmacother 2014;4 Suppl 3:107-13.

Andrade Cetto A, Becerra Jimenez J, Cardenas Vazquz R. Alpha

glucosidase inhibiting activities of some Mexican plants used for

treatment of Type 2diabetes. J Ethanopharmacol 2008;116:27-32.

Noor A, Bansal BS, Vijaylakshmi MA. Current update on antidiabatic

bio molecule from Key traditional Indian medicinal plants. Curr Sci

;104:721-7.

Geetika Pant G, Sai JK, Babasaheb S, Reddy PR, Sibi G. In vitro

α-amylase and α-glucosidase inhibitor activities of Abutilon indicum

leaves. Asian J Pharm Clin Res 2013;6 Suppl 5:22-4.

Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A.

In vitro study on a-amylase inhibitory activity of an Indian medicinal

plant, Phyllanthus amarus. Indian J Pharmacol 2010;42(5):280-2.

Karthic K, Kirthiram KS, Sadasivam S, Thayumanavan B. Identification

of alpha amylase inhibitors from Syzygium cumini Linn seeds. Indian J

Exp Biol 2008;46(9):677-80.

Kim JS, Kwon CS, Son KH. Inhibition of alpha-glucosidase and

amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem

;64(11):2458-61.

Published

2016-03-01

How to Cite

Dutta, J., and K. Mc. “IN VITRO HYPOGLYCAEMIC EVALUATION OF SEVEN CULINARY PLANTS OF NORTH EAST INDIA AGAINST TYPE 2 DIABETES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, Mar. 2016, pp. 209-12, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10416.

Issue

Section

Original Article(s)