• Cerquera Ospina Cc
  • Murillo Perea e



The essential oil isolated by hydrodistillation from peel of Citrus aurantium and Swinglea glutinosa, collected from Ibagué-Province of Tolima-Colombia (South America), was analyzed by GC/MS. The major compounds identified in essential oil of S. glutinosa were germacrene-D (30.8%), β-pinene (22.6%) and sabinene (11.6%), while limonene (94.4%)was identified as the most abundant component in C. aurantium.The ability of these products and mixturesto control cellular oxidation and measure theiranti-epimastigotes of Trypanosoma cruzi activity, the protozoan Chagas transmitter,were investigated.Besides, the type ofdrug interactionbetweenessential oil mixtureswas determinedby the values ofcombination index, which indicated for all cases the existence of a strong antagonism between the mixtures prepared.According to ABTS assay, β-carotene bleaching test andprotective activityagainst erythrocytes degradationshowed that volatiles compounds have a wide spectrum as cellular oxidation controller.This is the first time, that oils of S. glutinosa and C. aurantium, have high activity against T. cruzi, in vitro; capacity that could be directly associated to the chemotype of they, one of which (Swinglea glutinosa) appears not to be found in other research.



Essential oil composition, Citrusaurantium, Swingleaglutinosa, ABTS, Rutaceae.




Matheus D, Pulido X, Gutierrez A, Orduz J. Economic evaluation of

citrus fruit grown in the foothills of the Meta department for 12 years.

Rev Orinoquia 2014;14(l):16-26.

Espina L, Somolinos M, Loreen S, Conchello P, Garcia D, Pageen R.

Chemical composition of commercial citrus fruit essentials oils and

evaluation o their antimicrobial activity alone or in combined processes.

Food Control 2010;22:896-902.

Goulas V, Manganaris G. Exploring the phytochemical content and

the antioxidant potential of citrus fruits grown in cyprus. Food Chem


Manthey J, Guthrie N. Antiproliferative activities of citrus

flavonoids against six human cancer cell lines. J Agric Food Chem


Menichini F, Loizzo M, Bonesi M, Conforti F, De Luca D, Statti G,

et al. Phytochemical profile, antioxidant, anti-inflammatory and

hypoglycemic potential of hydroalcoholic extracts from Citrus

Medica L. Cv diamante flowers, leaves and fruits at two maturity

stages. Food Chem Toxicol 2011;49:1549-55.

Aruoma OI, Landes B, Ramful-Baboolall D, Bourdon E,

Neergheen-Bhujun V, Wagner KH, et al. Functional benefits of citrus

fruits in the management of diabetes. Prev Med 2012;54 Suppl: S12-6.

Lima LM, Perazzo FF, Tavares Carvalho JC, Bastos JK. Antiinflammatory


analgesic activities of

the ethanolic extracts






and stem






Orwa JA, Ngeny L, Mwikwabe NM, Ondicho J, Jondiko IJ. Antimalarial

and safety evaluation of extracts from Toddalia asiatica (L) Lam.

(Rutaceae). J Ethnopharmacol 2013;145:587-90.

Casado M, Celia M, Gutiérrez Y, Rodríguez E. Acercamiento al género

Murraya (Rutaceae) y a la especie Murraya paniculata (L.) Jack. Rev

Cubana Plant Med 2011;16:408-18.

Gounaris Y. Biotechnology for the production of essential oils, flavours

and volatile isolates. Flavour Fragr J 2010;25(5):367-86.

Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of

essential oils – A review. Food Chem Toxicol 2008;46(2):446-75.

Ibrahim MA, Kainulainen P, Aflatuni A, Tiilikkala K, Holopainen JK.

Insecticidal, repellent, antimicrobial activity and phytotoxicity of

essential oils: With special reference to limonene and its suitability for

control of insect pests. Agric Food Sci Finland 2001;10:243-59.

Reichling J, Schnitzler P, Suschke U, Saller R. Essential oils of

aromatic plants with antibacterial, antifungal, antiviral, and cytotoxic

properties - An overview. Forsch Komplementmed 2009;16(2):79-90.

Desjeux P. Leishmaniasis. Nat Rev Microbiol 2004;2(9):692.

Ndao M, Spithill TW, Caffrey R, Li H, Podust VN, Perichon R, et al.

Identification of novel diagnostic serum biomarkers for Chagas’ disease

in asymptomatic subjects by mass spectrometric profiling. J Clin

Microbiol 2010;48(4):1139-49.

Gürtler RE, Segura EL, Cohen JE. Congenital transmission of

Asian J Pharm Clin Res, Vol 9, Issue 2, 2016, 213-219

Caleño et al.

Trypanosoma cruzi infection in Argentina. Emerg Infect Dis


Nóbrega AA, Garcia MH, Tatto E, Obara MT, Costa E, Sobel J, et al.

Oral transmission of Chagas disease by consumption of açaí palm fruit,

Brazil. Emerg Infect Dis 2009;15(4):653-5.

PAHO. Estimación Cuantitativa de la Enfermedad de Chagas en Las

Americas; 2006. Available from:


Pérez G, Ramos M, Sánchez M. Fresán O, Pérez J. Antiprotozoa

activity of some essential oils. J Med Plants Res 2012;6(15):2901-8.

Upcroft JA, Campbell RW, Benakli K, Upcroft P, Vanelle P. Efficacy

of new 5-nitroimidazoles against metronidazole-susceptible

and - Resistant Giardia, Trichomonas, and Entamoeba spp. Antimicrob

Agents Chemother 1999;43(1):73-6.

Upcroft P, Upcroft JA. Drug targets and mechanisms of resistance in the

anaerobic protozoa. Clin Microbiol Rev 2001;14(1):150-64.

Tirzitis G, Bartosz G. Determination of antiradical and antioxidant

activity: Basic principles and new insights. Acta Biochim Pol


Rojas J, Ronceros S, Palacios O. In vitro anti-Trypanosoma Cruzi

activity of essential oils of ten medicinal plants. Anal Facultad De Med


Adams R. Identification of Essential Oil Components by Gas

Chromatography/Mass Spectrometry. Carol Stream, IL: Allured

Publishing Corporation; 2007.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C.

Antioxidant activity applying an improved ABTS radical cation

decolorization assay. Free Radic Biol Med 2009;26(9):1231-7.

Henao L, Mejía L. Evaluación del efecto antioxidante de aceites

esenciales y extractos de Eugenia carryophyla, Origanum vulgare y

Thymus vulgaris. Biosalud 2009;8:58-70.

Thephinlap C, Pangjit K, Suttajit M, Srichairatanakool S. Anti-oxidant

properties and anti-hemolytic activity of Psidium guajava, Pandanous

odorus and Rhinacanthus nasutus. J Med Plants Res 2013;7(27):2001-9.

Araya JE, Neira I, da Silva S, Mortara RA, Manque P, Cordero E,

et al. Diterpenoids from Azorella compacta (Umbelliferae) active on

Trypanosoma cruzi. Mem Inst Oswaldo Cruz 2003;98(3):413-8.

Chou TC. Drug combination studies and their synergy quantification

using the Chou-Talalay method. Cancer Res 2010;70(2):440-6.

Murillo E, Fernández K, Sierra D, Viña A. Physical-chemical

characterization of essential oil of Basil II. Rev Colombiana Química


Peez M, Hernandez H, Hernandez S. Antioxidant activity of Tagetes

erecta essential oil. J Chil Chem Soc 2006;51(2):883-6.

Zhang Q, Schlyter F. Olfactory recognition and behavioural avoidance

of angiosperm nonhost volatiles by conifer-inhabiting bark beetles.

Agric Forest Entomol 2004;6(1):1-20.

Bruneton J, del Fresno VA, Acame CE, Lizabe RM. Farmacognosia.

ed. Zaragoza: Acribia; 2001. p. 134.

Wildman R. Handbook of Nutraceuticals and Functional Foods. 2



USA: CRC Press; 2000. p. 250.

Noma Y, Asakawa Y. Metabolic pathways of monoterpenoids by

microorganisms. Curr Top Phytochem 2000;4:63-78.

Yoo SK, Day DF, Cadwallader KR. Bioconversion of α- and β-pinene

by Pseudomonas sp. strain PIN. Process Biochem 2001;36:925-32.

Murillo E, Viña A. Determination of volatiles constituents Basil

(Ocimum spp) using two methods of extraction. Rev Colombiana

Química 1999;28(1):65-74.

Sarrou E, Chatzopoulou P, Dimassi-Theriou K, Therios I. Volatile

constituents and antioxidant activity of peel, flowers and leaf oils of Citrus


aurantium L. growing in Greece. Molecules 2013;18(9):10639-47.

Bueno J, Martínez J, Stashenko E, Ribón W. Anti-tubercular activity

of eleven aromatic and medicinal plants occurring in Colombia.

Biomédica 2009;29(1):51-60.

Jaramillo-Colorado B, Olivero-Verbel J, Stashenko EE, WagnerDöbler


Kunze B.



activity of

essential oils



plants. Nat Prod Res 2012;26(12):1075-86.

Díaz C, Arrazóla G, Ortega F, Gaviria J. Characterization of essential

oil of swinglea lemon (Swinglea glutinosa) peel for GC-ME. Temas

Agrarios 2005;10:2-28.

Long L, Kwee D, Halliwell B. The antioxidant activities of

seasonings used in Asian cooking. Powerful antioxidant activity of

dark soy sauce revealed using the ABTS assay. Free Radical Res


Berenholtz S, Lehmann C, Pronovost P, Murphy D. RBC transfusion

practices in two ICUs: A mixed methods assessment of knowledge

translation. Am J Respir Crit Care Med 2012;185:60-5.

Frankel E, Meyer A. The problems of using one-dimensional methods

to evaluate multifunctional food and biological antioxidants J Sci Food

Agric 2000;80(13):1925-41.

Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic

compounds of 112 traditional Chinese medicinal plants associated with

anticancer. Life Sci 2004;74(17):2157-84.

Murali R, Karthikeyan A, Saravanan R. Protective effects of D-limonene

on lipid peroxidation and antioxidant enzymes in streptozotocininduced

diabetic rats. Basic Clin Pharmacol Toxicol


Morrison B. Organic Chemistry. New York: Pearson; 1985.

Escobar P, Herrera L, Leal S, Durán C, Stashenko E. Chemical

composition and anti-tripanosomal activity of essential oils from

Tagetes (Asteraceae Fam.) grown in Colombia. Rev Univ Ind Santander


Sáchez B. Perspectivas de la Terapia Antichagásica. Master’s Thesis.

México: Colima´s University; 2007.

Dorman HJ, Deans SG. Antimicrobial agents from plants: Antibacterial

activity of plant volatile oils. J Appl Microbiol 2000;88(2):308-16.

Burt S. Essential oils: Their antibacterial properties and potential

applications in foods – A review. Int J Food Microbiol 2004;94(3):223-53.

Ultee A, Kets EP, Smid EJ. Mechanisms of action of carvacrol on

the food-borne pathogen Bacillus cereus. Appl Environ Microbiol


Calsamiglia S, Busquet M, Cardozo PW, Castillejos L, Ferret A. Invited

review: Essential oils as modifiers of rumen microbial fermentation.

J Dairy Sci 2007;90(6):2580-95.

Nguemtchouin M, Ngassoum M, Ngamo L, Gaudu X, Cretin M.

Insecticidal formulation based on Xylopia aethiopica essential oil and

kaolinite clay for maize protection. Crop Protect 2010;29(9):985-91.

Moreira I, Lago J, Young M, Roque F. Antifungal aromadendrane

sesquiterpenoids from the leaves of Xylopia brasiliensis. J Braz Chem

Soc 2013;14(5):828-31.

Santoro GF, Cardoso MG, Guimarães LG, Mendonça LZ, Soares MJ.

Trypanosoma cruzi: Activity of essential oils from Achillea

millefolium L. Syzygium aromaticum L. and Ocimum basilicum L. on

epimastigotes and trypomastigotes. Exp Parasitol 2007;116:283-90.

Grassmann J, Hippeli S, Spitzenberger R, Elstner EF. The monoterpene

terpinolene from the oil of Pinus mugo L. in concert with alphatocopherol

and beta-carotene effectively

prevents oxidation of LDL.



Milde J, Elstner EF, Grassmann J. Synergistic inhibition of low-density

lipoprotein oxidation by rutin, gamma-terpinene, and ascorbic acid.

Phytomedicine 2004;11(2-3):105-13.



How to Cite

JL, C. C. ., C. Ospina Cc, M. A. W, M. A. . JJ, and M. Perea e. “APPLICATION OF ESSENTIAL OILS FROM TWO SPECIES OF THE RUTACEAE FAMILY AS CELLULAR OXIDATION CONTROLLER AGENT AND TRYPANOCIDAL CAPACITY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, Mar. 2016, pp. 213-9,



Original Article(s)