TOXICITY ASSESSMENT OF CARBON NANOTUBES ON ERYTHROCYTE MORPHOLOGY AND LYMPHOCYTES IN VITRO
Objectives: Carbon nanomaterials have been used in many biomedical applications due to its unique physical properties. Functionalization of carbon
nanotubes (CNT) could improve the physical properties, but pharmacokinetics and toxic effects of pristine and functionalized CNTs, are not well
defined. In this study, the toxicity of the pristine and hydroxyl group functionalized Multiâ€‘Walled CNT (MWCNT) compared.
Methods: MWCNTs was interacted with human blood. The induced morphological changes in the erythrocytes and cytotoxicity were observed at
different concentrations. A thin blood smear was prepared, and the erythrocyte images were obtained using a digital microscope. MTT assay used to
assess the cytotoxicity.
Results: The result indicate that the pristine MWCNT caused more toxic effects than â€“OH functionalized MWCNT, which was assessed in terms of
changes in the morphology of the erythrocytes and cytotoxicity caused to the lymphocyte cells.
Conclusion: The functionalization of MWCNT could reduce the hemotoxicity and improves the biocompatibility.
Keywords: Carbon nanotubes, Cytotoxicity, Echinocytes, Erythrocyte, Multiâ€‘walled carbon nanotubes, Lymphocyte.
Sarikaya M, Tamerler C, Jen AK, Schulten K, Baneyx F. Molecular
biomimetics: Nanotechnology through biology. Nat Mater
Zhang S. Fabrication of novel biomaterials through molecular
selfâ€‘assembly. Nat Biotechnol 2003;21(10):1171â€‘8.
Browne WR, Feringa BL. Making molecular machines work. Nat
Vittorio O, Duce SL, Pietrabissa A, Cuschieri A. Multiwall
carbon nanotubes as MRI contrast agents for tracking stem cells.
MÃ©nardâ€‘Moyon C, Kostarelos K, Prato M, Bianco A. Functionalized
carbon nanotubes for probing and modulating molecular functions.
Chem Biol 2010;17(2):107â€‘15.
Massoud TF, Gambhir SS. Molecular imaging in living subjects:
Seeing fundamental biological processes in a new light. Genes Dev
Vardharajula S, Ali SZ, Tiwari PM, Eroglu E, Vig K, Dennis VA,
et al. Functionalized carbon nanotubes: Biomedical applications. Int J
Naya M, Kobayashi N, Endoh S, Maru J, Honda K, Ema M, et al. In vivo
genotoxicity study of singleâ€‘wall carbon nanotubes using comet assay
following intratracheal instillation in rats. Regul Toxicol Pharmacol
Shvedova AA, Kisin ER, Porter D, Schulte P, Kagan VE, Fadeel B, et al.
Mechanisms of pulmonary toxicity and medical applications of carbon
nanotubes: Two faces of Janus? Pharmacol Ther 2009;121(2):192â€‘204.
Awasthi KK, John PJ, Awasthi A, Awasthi K. Multi walled carbon
nano tubes induced hepatotoxicity in Swiss albino mice. Micron
Vales G, Rubio L, Marcos R. Genotoxic and cellâ€‘transformation
effects of multiâ€‘walled carbon nanotubes (MWCNT) following in vitro
subâ€‘chronic exposures. J Hazard Mater 2015;306:193â€‘202.
Oliveira SF, Bisker G, Bakh NA, Gibbs SL, Landry MP, Strano MS.
Protein functionalized carbon nanomaterials for biomedical
applications. Carbon 2015;95:767â€‘79.
Helland A, Wick P, Koehler A, Schmid K, Som C. Reviewing the
environmental and human health knowledge base of carbon nanotubes.
Cien Saude Colet 2008;13:441â€‘52.
Rodak BF, Carr JH. Introduction to blood smear examination. Clinical
Hematology Atlas. 4
ed., Ch. 1. St. Louis: Elsevier Health Sciences;
Jerobin J, Sureshkumar RS, Anjali CH, Mukherjee A, Chandrasekaran N.
Biodegradable polymer based encapsulation of neem oil nanoemulsion
for controlled release of Azaâ€‘A. Carbohydr Polym 2012;90(4):1750â€‘6.
Singh M, Shin S. Changes in erythrocyte aggregation and deformability
in diabetes mellitus: A brief review. Indian J Exp Biol 2009;47(7):7â€‘15.
Shinto H, Fukasawa T, Yoshisue K, Tezuka M, Orita M. Cell membrane
disruption induced by amorphous silica nanoparticles in erythrocytes,
lymphocytes, malignant melanocytes, and macrophages. Adv Powder
Li SQ, Zhu RR, Zhu H, Xue M, Sun XY, Yao SD, et al. Nanotoxicity
of TiO2 nanoparticles to erythrocyte in vitro. Food Chem Toxicol
Yuvraj V, Indumathi J, Singh M. Effects of cigarette smoking on
morphology and aggregation of erythrocytes. Clin Hemorheol
Holder AL, Gothâ€‘Goldstein R, Lucas D, Koshland CP. Particleâ€‘induced
artifacts in the MTT and LDH viability assays. Chem Res Toxicol
Lucia UC, Delia C, Aureliano C, Maria FA, Raffaele M, Buresti G,
et al. Cytotoxic, genotoxic and proinflammatory response of human
bronchial cells to pristine and functionalized MWCNTs. Mater Today
Hall JE. Unit VI blood cells, immunity and blood coagulation. Guyton
and Hall Text Book of Medical Physiology. 13th ed. Philadelphia, PA:
Elsevier Health Sciences. 2015. p. 474â€‘6.
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