HISTOLOGICAL EFFECT OPTIMIZATION COMBINATION OF BACILLUS SPHAERICUS 2362 AND BACILLUS THURINGIENSIS SUBSP. ISRAELENSIS IN MIDGUT OF CULEX QUINQUEFASCIATUS RESISTANCE
Objective: The objective of the study was to analyze poreâ€‘forming at Culex quinquefasciatus resistant larvae midgut and analyze optimization ratio
after treatment using combination Bacillus thuringiensis var. israelensis (Bti) and Bacillus sphaericus (Bs) 2362.
Methods: This research was an experimental study. C. quinquefasciatus larvae were divided into 10 groups. The Group I until VII had treatment by
the various concentration of combination Bti and Bs 2362, treatment Group VIII as a positive control, Group IX as a negative control, and Group X as
a single Bs 2362. All of the treatment groups were examined for the histological effect of C. quinquefasciatus larvae midgut by hematoxylin eosin. The
lowest lethal concentration 50% (LC
Results: The lowest LC
) was a standard optimization ratio of combination Bti and Bs 2362. LC
was analyzed by probit.
was 2.274 part a million (ppm) at Group I was the optimization ratio. Various combination treatments Group I until VII were
shown pores at C. quinquefasciatus larvae midgut after treatment by a combination of Bti and Bs 2362.
Conclusion: Combination of Bti and Bs 2362 was shown pores at C. quinquefasciatus larvae midgut, and optimization ratio was shown in Group I.
Keywords: Bacillus thuringiensis var. israelensis, Bacillus sphaericus 2362, Culex quinquefasciatus midgut.
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