THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS

  • Maha Z Rizk
  • HANAN F ALY
  • Azza A Matloub
  • Ghadha I Fouad

Abstract

Objective: This study aims to evaluate the relation between hypercholesterolemia and aging, beside the role of Ulva fasciata polysaccharides (UFP)
aqueous extracts in lowering cholesterol in aged hypercholesterolemia-induced rats was demonstrated.
Method: A total of 140 male Wister rats weighing 120±10 g, 6-9 months old were used. Hypercholesterolemia was induced in rats by feeding rats
high-fat diet (cholesterol), cholesterol was orally administrated at a dose of (30 mg/0.3 ml olive oil/1 kg animal) 5 times a week for 12 consecutive
weeks, lard fat was mixed with normal diet (1 kg of animal lard was added to 5 kg of normal diet), the occurrence of hypercholesterolemia was
determined by measuring the lipid profile (TC, LDL-C, high-density lipoprotein-cholesterol [HDL-C], triglyceride [TG]), the old hypercholesterolemic
(HC) rats were only used.
Results: The antihypercholesterolemic (HC) effects of ulvan, the sulfated polysaccharide extracted from the green alga Ulvafasciata, in aged rats,
were studied. Algal treatment declared a significant reduction in serum total lipid level while, elevation of high-density lipoprotein-cholesterol level
was noticed in HC rats. Moreover, the algal treatment significantly decreased serum liver and kidney functions biomarkers and improved the hepatic
antioxidant levels in hyperlipidemic aged rats. In addition, ulvan administration significantly suppressed the expression of tumor necrosis factoralpha,
myeloperoxidase
and cell vascular
and intracellular
adhesion
molecules-1,
while
increased
the anti-inflammatory
cytokine
level;
interleukin-10.
Furthermore,
the histopathological
examination
of aorta,
liver
and
kidney
of HC-treated
rats
indicated
that
the Ulva
fasciata
polysaccharides
(UFP),
is

a
potent
natural
hypolipidemic
nutraceutical
for
the amelioration
of hyperlipidemia
in aged rats.
Conclusion: It could be concluded that, in comparison with the standard anti-HC drug (fluvastatin) used in this study, both cold and hot UFP algal
extracts of U. fasciata demonstrated appreciable anti-hypercholesterolemic property, in addition to their antioxidant activity even in the old HC
stressed rats. Thus, it could be used as a natural lipid regulator.
Keywords: Ulvafasciata, Hypercholesterolemia, Polysaccharides, Rats, Sulfated polysaccharides, Aging, Hyper-cholesterolemia.

References

REFERENCES
1. Parini P, Angelin B, Rudling M. Cholesterol and lipoprotein metabolism
in aging: Reversal of hypercholesterolemia by growth hormone
174
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 165-176
Rizk et al.
treatment in old rats. Arterioscler Thromb Vasc Biol 1999;19(4):832-9.
2. Gälman C, Matasconi M, Persson L, Parini P, Angelin B, Rudling M.
Age-induced hypercholesterolemia in the rat relates to reduced
elimination but not increased intestinal absorption of cholesterol. Am
J Physiol Endocrinol Metab 2007;293(3):E737-42.
3. Trapani L, Pallottini V. Age-related hypercholesterolemia and
HMG-CoA reductase dysregulation: Sex does matter (A gender
perspective). Curr Gerontol Geriatr Res 2010;2010:7.
4. Corpas E, Harman SM, Blackman MR. Human growth hormone and
human aging. Endocr Rev 1993;14(1):20-39.
5. Brown MS, Goldstein JL. A proteolytic pathway that controls the
cholesterol content of membranes, cells, and blood. Proc Natl Acad Sci
USA 1999;96(20):11041-8.
6. Espenshade PJ, Hughes AL. Regulation of sterol synthesis in
eukaryotes. Annu Rev Genet 2007;41:401-27.
7. Choi YS, Ide T, Sugano M. Age-related changes in the regulation of
cholesterol metabolism in rats. Exp Gerontol 1987;22(5):339-49.
8. Wilhelmsen L, Johansson S, Rosengren A, Wallin I, Dotevall A,
Lappas G. Risk factors for cardiovascular disease during the period
1985-1995 in Göteborg, Sweden. The GOT-MONICA project. J Intern
Med 1997;242(3):199-211.
9. Parini P, Angelin B, Rudling M. Cholesterol and lipoprotein metabolism
in aging: Reversal of hypercholesterolemia by growth hormone
treatment in old rats. Arterioscler Thromb Vasc Biol 1999;19(4):832-9.
10. Marino M, Pallottini V, D’Eramo C, Cavallini G, Bergamini E,
Trentalance A. Age-related changes of cholesterol and dolichol
biosynthesis in rat liver. Mech Ageing Dev 2002;123(8):1183-9.
11. Pallottini V, Martini C, Cavallini G, Bergamini E, Mustard KJ,
Hardie DG, et al. Age-related HMG-CoA reductase deregulation
depends on ROS-induced p38 activation. Mech Ageing Dev
2007;128(11-12):688-95.
12. Harman D. Aging: A theory based on free radical and radiation
chemistry. J Gerontol 1956;11(3):298-300.
13. Stadtman ER. Protein oxidation and aging. Free Radic Res
2006;40(12):1250-8.
14. Lammert F, Wang DQ. New insights into the genetic regulation of
intestinal cholesterol absorption. Gastroenterology 2005;129(2):718-34.
15. Wang DQ. Aging per se is an independent risk factor for cholesterol
gallstone formation in gallstone susceptible mice. J Lipid Res
2002;43(11):1950-9.
16. Tziomalos K, Athyros VG, Karagiannis A, Mikhailidis DP.
Management of statin-intolerant high-risk patients. Curr Vasc
Pharmacol 2010;8(5):632-7.
17. Qi HM, Huang LY, Liu XL, Liu DM, Zhang QB, Liu SM.
Antihyperlipidemic activity of high sulfate content derivative of
polysaccharide extracted from Ulva pertusa (Chlorophyta). Carbohydr
Polym 2012;87(2):1637-40.
18. Sathivel A, Raghavendran HR, Srinivasan P, Devaki T. Antiperoxidative
and
anti-hyperlipidemic
nature
of
Ulva
lactuca
crude
polysaccharide
on
D-galactosamine induced
hepatitis in rats. Food
Chem
Toxicol
2008;46(10):3262-7.
19. Qi HM, Zhao TT, Zhang QB, Li ZE, Zhao ZQ, Xing RE. Antioxidant
activity of different molecular weight sulfated polysaccharides from
Ulva pertusa Kjellm (Chlorophyta). J Appl Phycol 2005;17:527-34.
20. Pengzhan Y, Quanbin Z, Ning L, Zuhong X, Yanmei W, Zhïen L.
Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary
studies on their anti-hyperlipidemia activity. J Appl Phycol
2003;15:21-7.
21. Adaramoye OA, Akintayo O, Achem J, Fafunso MA. Lipid-lowering
effects of methanolic extract of Vernonia amygdalina leaves in rats fed
on high cholesterol diet. Vasc Health Risk Manag 2008;4(1):235-41.
22. Koter M, Broncel M, Chojnowska-Jezierska J, Klikczynska K,
Franiak I. The effect of atorvastatin on erythrocyte membranes and
serum lipids in patients with type-2 hypercholesterolemia. Eur J Clin
Pharmacol 2002;58(8):501-6.
23. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic
determination of total serum cholesterol. Clin Chem 1974;20(4):470-5.
24. ZÖllner N, Kirsch K. Colorimetric method for determination of total
lipids. J Expl Med 962;135:545-550.
25. Fassati P, Prencipe L. Serum triglycerides determined colorimetrically
with an enzyme that produces hydrogen peroxide. Clin Chem
1982:28(10):2077-80.
26. Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol
determination in high-density lipoproteins separated by three different
methods. Clin Chem 1977;23(5):882-4.
27. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the
concentration of low-density lipoprotein cholesterol in plasma, without
use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.
28. Norbert WT. Clinical Guide to Laboratory Tests. 3
ed. Philadelphia, PA:
W.B. Saunders Company; 1995.
rd
29. Reitman S, Frankel S. A colorimetric method for the determination of
serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J
Clin Pathol 1957;28(1):56-63.
30. Belfield A, Goldberg DM. Colorimetric determination of alkaline
phosphatase activity. Enzyme 1971;12:561-68.
31. Walters M, Gerade H. Ultramicro-method for the determination
of conjugated and total bilirubin in serum or plasma. Microchem
J 1970;15(2):231-43.
32. Bradford MM. A rapid and sensitive method for the quantitation of
microgram quantities of protein utilizing the principle of protein-dye
binding. Anal Biochem 1976;72:248-54.
33. Doumas BT, Watson WA, Biggs HG. Albumin standards and the
measurement of serum albumin with bromcresol green. Clin Chim Acta
1971;31:87-96.
34. Trinder P. Determination of glucose in blood using glucose oxidase
with an alternative oxygen acceptor. Ann Clin Biochem 1969;6(1):24-5.
35. Beutler E, Duron O, Kelly BM. Improved method for the determination
of blood glutathione. J Lab Clin Med 1963;61:882-8.
36. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined
by a new colorimetric method. Clin Chim Acta 1978;90(1):37-43.
37. Fawcett JK, Scott JE. A rapid and precise method for the determination
of urea. J Clin Pathol 1960;13:156-9.
38. Schirmeister J. Determination of creatinine level. Dtsch Med Wschr
1964:89:1940-7.
39. Carlson SE, Mitchell AD, Carter ML, Goldfarb S. Evidence that
physiologic levels of circulating estrogens and neonatal seximprinting
modify
postpubertal hepatic
microsomal
3-hydroxy-3methylglutaryl
coenzyme
A
reductase activity.
Biochim Biophys
Acta

1980;633(2):154-61.
40. Borai H, Ezz KM, Rizk MZ, et al. Hypolipidemic and Anti- atherogenic
Effect of Sulphated Polysaccharidesfrom the Green Alga Ulva fasciata.
Int J Pharm Sci Rev Res 2015;31(1)1-12.
40. Sathivel A, Raghavendran HR, Srinivasan P, Devaki T. Antiperoxidative
and
anti-hyperlipidemic
nature
of
Ulva
lactuca
crude
polysaccharide
on
D-galactosamine induced
hepatitis in rats. Food
Chem
Toxicol
2008;46(10):3262-7.
41. Castro R, Piazzon MC, Zarra I, Leiro J, Noya M, Lamas J. Stimulation
of turbot phagocytes by Ulva rigida C. Agardh polysaccharides.
Aquaculture 2006;254:9-20.
41. Levine GN, Keaney JF, Vita JA. Cholesterol reduction in cardiovascular
disease-clinical benefits and possible mechanisms. N Engl J Med.
1995;332:512-21.
42. Parini P, Angelin B, Rudling M. Cholesterol and lipoprotein
metabolism in aging: Reversal of hypercholesterolemia by growth
hormone treatment in old rats. Arterioscler Thromb Vasc Biol
1999;19(4):832-9.
43. Matasconi M, Parini P, Angelin B, Rudling M. Pituitary control of
cholesterol metabolism in normal and LDL receptor knock-out mice:
Effects of hypophysectomy and growth hormone treatment. Biochim
Biophys Acta 2005;1736(3):221-7.
44. Rudling M, Parini P, Angelin B. Growth hormone and bile acid
synthesis. Key role for the activity of hepatic microsomal cholesterol
7alpha-hydroxylase in the rat. J Clin Invest 1997;99(9):2239-45.
45. Lusis AJ. Atherosclerosis. Nature 2000;407(6801):233-41.
46. Stalenhoef AF, De Graaf J. Association of fasting and non-fasting
serum triglycerides with cardiovascular disease and the role of
remnant-like lipoproteins and small dense LDL-C. Curr Opin Lipidol
2008;19(4):355-61.
47. Godard M, De´corde K, Ventura E, Soteras G, Baccou JC, Cristol JP,
et al. Polysaccharides from the green alga Ulva rigida improve the
antioxidant status and prevent fatty streak lesions in the high cholesterol
fed hamster, an animal model of nutritionally-induced atherosclerosis.
Food Chem 2009;115(1):176-80.
48. Noori S, Zafar H, Mahboob T. Biochemical effectiveness of cocoa
powder on electrolytes homeostasis, liver and cardiac specific enzymes
and renal function. Pak J Nutr 2009;8:882-6.
49. Nyblom H, Berggren U, Balldin J, Olsson R. High AST/ALT ratio may
indicate advanced alcoholic liver disease rather than heavy drinking.
Alcohol Alcohol 2004;39(4):336-9.
50. Sudhahar V, Kumar SA, Sudharsan PT, Varalakshmi P. Protective
effect of lupeol and its ester on cardiac abnormalities in experimental
hypercholesterolemia. Vascul Pharmacol 2007;46(6):412-18.
51. Kim AR, Lee JJ, Lee YM, Jung HO, Lee MY. Cholesterol-lowering and
anti-obesity effects of Polymnia sonchifolia Poepp. and Endl. Powder
175
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 165-176
Rizk et al.
in rats fed a high fat-high cholesterol diet. J Korean Soc Food Sci Nutr
2010;39(2):210-8.
52. Awada NE, Hamed MA, Seida AA, Elbatanony MM. Efficacy of Ficus
spp. on renal injury induced by hypercholesterolemia. Nat Prod Res
2012;26(16):1561-4.
53. IghodaroO.M, Omole JO, Adejuwon AO, Odunaiya AA. Effects of
Parinari polyandra seed extract on blood glucose level and biochemical
indices in wistar rats. Int J Diabetes Res 2012;1(4):68-72.
54. Herreo M, Cifuentes A, Ibanez E. Sub- and supercritical fluid extraction
of functional ingredients from different natural sources: Plants, foodby-products,
algae and microalgae. Food Chem 2006;98:136-48.
55. Kopec KL, Burns D. Nonalcoholic fatty liver disease: A review of
the spectrum of disease, diagnosis, and therapy. Nutr Clin Pract
2011;26(5):565-76.
56. Zhang Q, Li N, Zhou G, Lu X, Xu Z, Li Z. In vivo antioxidant activity
of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in
aging mice. Pharmacol Res 2003;48(2):151-5.
57. Qi H, Zhang Q, Zhao T, Hu R, Zhang K, Li Z. In vitro antioxidant
activity of acetylated and benzoylated derivatives of polysaccharide
extracted from Ulva pertusa (Chlorophyta). Bioorg Med Chem Lett
2006;16(9):2441-5.
58. Sun H, Koike T, Ichikawa T, Hatakeyama K, Shiomi M, Zhang B,
et al. C-reactive protein in atherosclerotic lesions: Its origin and
pathophysiological significance. Am J Pathol 2005;167(4):1139-48.
59. Hadi HA, Carr CS, Al Suwaidi J. Endothelial dysfunction:
Cardiovascular risk factors, therapy, and outcome. Vasc Health Risk
Manag 2005;1(3):183-98.
60. Blankenberg S, Barbaux S, Tiret L. Adhesion molecules and
atherosclerosis. Atherosclerosis 2003;170(2):191-203.
61. Kamesh V, Sumathi T. Antihypercholesterolemic effect of Bacopa
monniera Linn. on high cholesterol diet induced hypercholesterolemia
in rats. Asian Pac J Trop Med 2012;5(12):949-55.
62. Posuwan J, Prangthip P, Leardkamolkarn V, Yamborisut U, Surasiang R,
Charoensiri R, et al. Long-term supplementation of high pigmented
rice bran oil (Oryza sativa L.) on amelioration of oxidative stress and
histological changes in streptozotocin-induced diabetic rats fed a high
fat diet; Riceberry bran oil. Food Chem 2013;138(1):501-8.
63. Rezq AA, El-Khamisy AE. Hypolipidemic and Hypocholestermic
effect of pine nuts in rats fed high fat, cholesterol-diet. World Appl Sci
J 2011;15(12):1667-77.
64. Jouyban A, Shoja MM, Ardalan MR, Khoubnasabjafari M, Sadighi A,
Tubbs RS, et al. The effect of quince leaf decoction on renal injury
induced by hypercholesterolemia in rabbits: A pilot study. J Med Plants
Res 2011;5(21):5291-5.
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How to Cite
Z Rizk, M., H. F. ALY, A. A. Matloub, and G. I Fouad. “THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 3, May 2016, pp. 165-76, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10835.
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