ELECTRO-OXIDATION OF NIMESULIDE AT GOLD ELECTRODE AND ITS DETERMINATION IN PHARMACEUTICAL DOSAGE FORM AND HUMAN BIOLOGICAL FLUID
The electro-oxidation of nimesulide has been investigated by cyclic and differential-pulse voltammetry at different pH at gold electrode. Cyclic voltammetric studies were performed in a wide range of sweep rates and various concentrations of nimesulide. The oxidation process was irreversible and exhibited a diffusion-controlled behavior. According to the linear relation between the peak current and the nimesulide concentration, differential-pulse voltammetric method for the quantitative determination in pharmaceuticals was developed. The recovery was determined to be 97.3 to 100.2 % by means of standard addition method. The linear response was obtained in the range of 2.0 x 10-7 to 1.2 x 10-6 M with a detection limit of 1.11 x 10-9 M with good selectivity and sensitivity. The electrochemical behaviors of nimesulide were studied and electron-transfer coefficient (Î± = 0.5), proton number (X = 1) and electron transfer number (n = 2) have been determined. The proposed method was also applied for the detection of nimesulide in urine as a real sample.Keywords: Nimesulide; Gold electrode; Electrochemical behavior; Drug analysis
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