• Jessy Shaji Department of Pharmaceutics, Prin. K. M. Kundnani College of Pharmacy, 23, Jote Joy Building, RambhauSalgaonkar Marg, Cuffe Parade, Mumbai 400005, India
  • Mahmood Shaikh Department of Pharmaceutics, Prin. K. M. Kundnani College of Pharmacy, 23, Jote Joy Building, RambhauSalgaonkar Marg, Cuffe Parade, Mumbai 400005, India


Objective: To prepare Nanoparticulate dosage form having improved drug bioavailability and reduced dosing frequency of antitubercular drugs
which will helps in improving patient compliance in the treatment of multi-drug resistant tuberculosis (MDR-TB).
Methods: Ionotropic gelation method was used to prepare D-cycloserine (D-CS)-loaded alginate-chitosan nanoparticles, and the particles are
characterized by their particle size and morphology using particle size analyzer and scanning electron microscopy (SEM). X-ray diffraction (XRD),
differential scanning calorimetry (DSC), and Fourier-transformed infrared (FTIR) studies were used to determine drug-polymer interactions and drug
entrapment. Entrapment efficiency, drug loading (DL), particle size, and zeta potential of nanoparticles were also studied. The 2
factorial designs of
experiments by Design-Expert
V9 were used to optimize the particle size and entrapment efficiency of nanoparticles.
Results: The optimized batch had shown the entrapment efficiency of 98.10±0.24% and DL of 69.32±0.44% with particle size and zeta potential
as 344±5 nm and −42±11.40 mV, respectively. DSC, FTIR, and XRD studies confirmed the drug entrapment within nanoparticle matrix. SEM results
showed spherical-shaped particles. Sustained release of drug from the nanoparticles was observed for 24 hrs period. Respirable fraction up to
52.37±0.7% demonstrates the formulation suitability for deep lung delivery. Lung inflammatory study showed a less inflammatory response.
Conclusion: Ionotropic gelation method can be used to prepare biocompatible particles with a high entrapment efficiency, DL, optimum particle size,
and controlled release characteristics, which can serve as a convenient delivery system for D-CS and could be a potential alternative to the existing
conventional therapy in MDR-TB.
Keywords: Nanoparticles, Alginate, Chitosan, Inhalation, Sustained release, Tuberculosis.

Author Biographies

Jessy Shaji, Department of Pharmaceutics, Prin. K. M. Kundnani College of Pharmacy, 23, Jote Joy Building, RambhauSalgaonkar Marg, Cuffe Parade, Mumbai 400005, India
Professor, Dept. of Pharmaceutics
Mahmood Shaikh, Department of Pharmaceutics, Prin. K. M. Kundnani College of Pharmacy, 23, Jote Joy Building, RambhauSalgaonkar Marg, Cuffe Parade, Mumbai 400005, India
M.Pharm Research Student, Dept. of Pharmaceutics


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How to Cite
Shaji, J., and M. Shaikh. “FORMULATION, OPTIMIZATION, AND CHARACTERIZATION OF BIOCOMPATIBLE INHALABLE D-CYCLOSERINE-LOADED ALGINATE-CHITOSAN NANOPARTICLES FOR PULMONARY DRUG DELIVERY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 8, Oct. 2016, pp. 82-95, doi:10.22159/ajpcr.2016.v9s2.11814.
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