• Irda Fidrianny School of Pharmacy Bandung Institute of Technology
  • Khoirunnisa Ayu Paramitha
  • Siti Kusmardiyani


Objectives: The goals of this research were to determine antioxidant activity from various leaves extracts of three cultivars of papaya using two
methods of antioxidant testing which were 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS),
and correlation of total phenolic content (TPC), total flavonoid content (TFC), and total carotenoid content (TCC) in various extracts of papaya leaves
with their IC
of DPPH and IC
of ABTS.
Methods: Extraction was carried out by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator.
Antioxidant activities using DPPH and ABTS assays, determination of TPC, TFC, and TCC were performed by ultraviolet-visible spectrophotometry
and correlation with their IC
Results: The lowest IC
of DPPH and IC
of ABTS scavenging activities were analyzed by Pearson's method.
of DPPH scavenging activity 0.84 µg/ml was given by ethanolic leaves extract of calina papaya, whereas ethanolic leaves
extract of Bangkok papaya showed the lowest IC
of ABTS scavenging activity 1.79 µg/ml. Ethanolic leaves extract of burung papaya had the highest
phenolic content and its ethyl acetate extract had the highest total flavonoid. There were significantly negative correlation between TPC in Bangkok
papaya and calina papaya extract with their IC
of DPPH.
Conclusions: All of the leaves extracts from three cultivars of papaya were categorized as very strong antioxidant by DPPH and ABTS methods.
Phenolic compounds in Bangkok papaya and calina papaya were the major contributor in IC
of DPPH scavenging activities. DPPH and ABTS gave no
linear result in antioxidant activities of leaves extract of three cultivars of papaya.
Keywords: 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Leaves, Papaya, Three cultivars.


1. Zielinski AA, Haminiuk CW, Alberti A, Nogueira A, Demiate IM,
Granato D. A comparative study of 7 the phenolic compounds and the
in vitro antioxidant activity of different Brazilian teas using multivariate
statistical techniques. Food Res Int 2014;60:246-54.
2. Xu BJ, Chang SK. Total phenolic content and antioxidant properties of
eclipse black beans (Phaseolus vulgaris L.) As affected by processing
methods. J Food Sci 2008;73:H19-27.
3. Mashkor IM. Phenolic content and antioxidant activity of fenugreek
seeds extract. Int J Pharmacogn Phytochem Res 2014;6(4):8s41-4.
4. Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities
of banana (Musa, AAA cv. Cavendish) fruits peel. Am J Biochem
Biotechnol 2005;1(3):125-31.
5. Sebei K, Gnouma A, Herchi W, Sakouhi F, Boukhchina S. Lipids,
proteins, phenolic composition, antioxidant and antibacterial activities
of seeds of peanuts (Arachis hypogaea L.) cultivated in Tunisia. Biol
Res 2013;46:257-63.
6. Fidrianny I, Windyaswari AS, Wirasutisna KR. Antioxidant capacities
of various leaves extract from five colors varieties of sweet potatoes
tubers using ABTS, DPPH assays and correlation with total flavonoid,
phenolic, carotenoid content. Res J Med Plant 2013;7(3):130-40.
7. Khokhar S, Magnusdottir SG. Total phenol, catechin, and caffeine
contents of teas commonly consumed in the United Kingdom. J Agric
Food Chem 2002;50:565-70.
8. Richelle M, Tavazzi I, Offord E. Comparison of the antioxidant activity
of commonly consumed polyphenolic beverages (coffee, cocoa, and
tea) prepared per cup serving. J Agric Food Chem 2001;49:3438-42.
9. Apak R, Güçlü K, Demirata B, Ozyürek M, Celik SE, Bektasoglu B,
et al. Comparative evaluation of various total antioxidant capacity
assays applied to phenolic compounds with the CUPRAC assay.
Molecules 2007;12:1496-547.
10. Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH.
Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating
antioxidant activity from guava fruit extracts. J Food Compos Anal
11. Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S,
Bianchi M, et al. Total antioxidant capacity of plant foods, beverages
and oils consumed in Italy assessed by three different in vitro assays.
J Nutr 2003;133(9):2812-9.
12. Imaga NA, Gbenle GO, Okochi VI, Adenekan S, Emmanuel TD,
Oyeniyi B, et al. Phytochemical and antioxidant nutrient constituents
of Carica papaya and Parquetina nigrescens extracts. Sci Res Essays
13. Maisarah AM, Nurul A, Asmah R, Fauziah O. Antioxidant analysis of
different parts of Carica papaya. Int Food Res J 2013;20(3):1043-8.
14. Blois MS. Antioxidant determination by the use of stable free radicals.
Nature 1958;181:1199-2000.
15. Bedawey AA. Characteristics of antioxidant isolated from some plants
sources. Food Nutr Sci 2010;1(1):5-12.
16. Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and
mechanism of protochatechuic acid in vitro. Funct Food Health Dis
17. Fidrianny I, Sukowati A, Sukrasno. In vitro antioxidant activities of
various leaves extracts from five varieties of Rambutan (Nephelium
lappaceum) and its correlation with total flavonoid, phenolic, carotenoid
content. Asian J Pharm Clin Res 2015;8(2):139-43.
18. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity,
phenol and flavonoid content of some selected Iranian medicinal plants.
Afr J Biotechnol 2006;5(11):1142-5.
19. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid
content in propolis by two complementary colorimetric methods.
J Food Drug Anal 2002;10(3):178-82.
20. del Castillo MD, Ames JM, Gordon MH. Effect of roasting on
the antioxidant activity of coffee brews. J Agric Food Chem
21. Zhou K, Wang H, Mei W, Li X, Luo Y, Dai H. Antioxidant activity of
papaya seed extracts. Molecules 2011;16:6179-92.
22. Addai ZR, Abdullah A, Mutalib SA, Musa KH, Douqan EM. Antioxidant
activity and physicochemical properties of mature papaya fruit (Carica
papaya L. cv. Eksotika). Adv J Food Sci Technol 2013;5(7):859-65.
23. Ozkan A, Gubbuk H, GuneÅŸ E, ErdoÄŸan A. Antioxidant capacity of
juice from different papaya (Carica papaya L.) cultivars grown under
greenhouse conditions in Turkey. Turk J Biol 2011;35:619-25.
24. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:
Chemistry, metabolism and structure-activity relationships. J Nutr
Biochem 2002;13:572-584.
25. Fidrianny I, Johan Y, Sukrasno. Antioxidant activities of different
polarity extracts from three organs of makrut lime (Citrus hystrix dc)
and correlation with total flavonoid, phenolic, carotenoid content.
Asian J Pharm Clin Res 2015;8(4):239-43.
26. Foote CS. Free Radicals in Biology. 3
ed. New York: Academic Press;
27. Beutner S, Bloedorn B, Hoffmann T, Martin HD. Synthetic singlet
oxygen quenchers. Methods Enzymol 2000;319:226-41.
28. Charles DJ. Antioxidant Properties of Spices Shells and Other. London:
John Willey; 2013.
29. Kobayashi M, Sakamoto Y. Singlet oxygen quenching ability of
astaxanthin esters from the green alga Haematococcus pluvialis.
Biotech Lett 1999;21(4):265-9.
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How to Cite
Fidrianny, I., K. A. Paramitha, and S. Kusmardiyani. “ANTIOXIDANT ACTIVITIES FROM VARIOUS LEAVES EXTRACTS OF THREE CULTIVARS OF PAPAYA FROM WEST JAVA-INDONESIA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 4, July 2016, pp. 299-03,
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