A REVIEW ON PRODUCTION OF PCDNA3.1-EGFP WITH CLONING PRINCIPLE METHOD


Ria Margiana

Abstract


ABSTRACT
pcDNA3.1-e green fluorescent protein (GFP) is an important compound that is already established and widely used as a marker in biomolecular
works. Producing pcDNA3.1-eGFP is not very complicated. It can be inserted to Escherichia coli and replicate in millions. Due to the availability of
E. coli is in Indonesia, this process should not be difficult at all. A description of the method of producing pcDNA3.1-eGFP will be covered so that
many Indonesian and other researchers as well can use it and develop it in their own research. The production of pcDNA3.1-eGFP has been done
by several researchers around the globe. In this review article, five relevant studies have been included. Their respective results were analyzed. The
pcDNA3.1 can be expressed differently apart from the eGFP form. Therefore, it can serve different purposes, especially when the different cloning and
sub-cloning processes are involved. It can be used to improve outcomes in various fields such as veterinary science, health, wellness, medicine, and
even agriculture. Through the use of the cloning principle, mass production of pcDNA3.1-eGFP can be carried out easily.
Keywords: pcDNA3.1-Green fluorescent protein, Cloning, Escherichia coli.


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References


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About this article

Title

A REVIEW ON PRODUCTION OF PCDNA3.1-EGFP WITH CLONING PRINCIPLE METHOD

Date

01-07-2016

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 9 Issue 4 July 2016 Page: 51-53

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

89 Views | 336 Downloads

Authors & Affiliations

Ria Margiana
Universitas Indonesia
Indonesia


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