IN VIVO ANTIDIABETIC POTENTIAL OF CYCLEA PELTATA IN STREPTOZOTOCIN‑INDUCED‑DIABETIC RATS

Authors

  • Sangeetha Ms
  • Priyanga S
  • Hemmalakshmi S
  • Devaki K

Abstract

 

Objective: Diabetes is one of the most common metabolic disorders in worldwide. Diabetes mellitus is a disorder of carbohydrate metabolism in
which sugars in the body are not oxidized to produce energy due to lack of the pancreatic hormone, insulin. This study was undertaken to find out the
antioxidant and antidiabetic potential of ethanolic root extract of Cyclea peltata.
Methods: The ethanolic root extract of C. peltata was administered to control and the experimental rats for 30 days. After the experimental period, the
animals were sacrificed and the serum, tissue samples were used for the determination of blood glucose level, protein, urea, creatinine, liver marker
enzymes, antioxidant enzymes, lipid profile, protein, lipid peroxidation and histopathological examination.
Results: The results of this study supports that the ethanolic root extract of C. peltata shows better antioxidant potential through which it exert
antidiabetic potential. Histopathology results also support the protective effect of C. peltata against streptozotocin induced diabetes.
Conclusion: The results scientifically confirm that the C. peltata can be used to treat various disorders caused by free radicals and other chemical
substances due to the presence of secondary metabolites that exert antioxidant and antidiabetic action.
Keywords: Antidiabetic, Antioxidants, Cyclea peltata, Lipid peroxidation.

References

Li F, Li Q, Gao D, Peng Y, Feng C. Properties and anti diabetic activity of polysaccharide from Porticulaca oleracea L. African Journal of Biotechnology 2009; 8: 569-573.

Patel SS, Shah RS, Goyal RK. Antihyperglcemic, antihyperlipidemic an antioxidant effects of Dihar, a polyhedral Ayurvedic formulation in Streptozotocin induced diabetic rats; Indian journal of Experimental Biology 2009; 47: 564-570.

Sachan NK, Yatidra K, Seema P, Thakur RN, Ganguvar SS, Kalaichelvan VK. Antidiabetic potential of Alcoholic and aqueous extracts of Ficus racemosa Linn. Bark in Normal and Alloxan induced Diabetic rats; International Journal of pharmaceutical Sciences and Drug Research 2009; 1: 24-27.

Ravi S, Sadashiva CT, Tamizmani T, Balasubramanian T, Rupesh kumar M, Balachandran I. In vitro glucose uptake by isolated rat hemi diaphargm study of Aegle marmelos correa root. Bangladesh pharmacol 2009; 4: 65-68.

Saravanan G, Leelavinothan P. Effects of Syzygium Cumini Bark on blood glucose, plasma insulin and C-peptide in streptozotocin -induced Diabetic rats. International journal of Endocrinal Metabolism 2006; 4: 96-105.

Dandu AM, Inamdar NM. Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of Andographis paniculata in streptozotocin induced diabetic rats. Pak. J.Pharm.Sci. 2009; 22: 49-52.

Ghosh T, Maity T, Sengupta P, Dash D, Bose A. Antidiabetic and Invivo antioxidant activity of Ethanolic extract of Bacopa Monneiri Linn. Aerial parts: A possible mechanism of action 2008; 7: 61-68.

Sunil C, Latha G, Mohanraj M, Kalichelvan K, Agastian P. ï¡-Glucosidase inhibitory and anti diabetic activities of ethanolic extract of Pisonia alba span leaves. International journal of Integrative Biology 2009; 12: 61-41.

Adeneya AA, Olagunju JA. Preliminary hypoglycemic and hypolipidemic activities of the Aqueous seed extract of Carica Papaya Linn. in wistar rats. Biology and Medicine 2009; 1: 1-10.

Vijaya Kirubha TSV, Senthamarai R, Mariya P and Mani P. Pharmacognostical and phytochemical standards of Cyclea peltata.(Lam) Hook.f & Thomson Leaves. Journal of Chemical and Pharmaceutical Research 2012; 4(3): 1465-1469.

Sasaki T, Matsy S, Sonae A. Effect of acetic acid concentration on colour reaction in O-Toluidine boric acid method for blood glucose estimation, Rinsho Kagaku 1972; 1: 346-353.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1952; 193: 265–275.

Misra HP, Fridovich A. Assay of superoxide dismutase. J Biol Chem 1972; 247: 3170–3175.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972, 47: 389–394.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973; 179: 588–590.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-Tranferase the first enzyme step in mercapturic acid formation. J BiolChem 1981; 249: 7130–7139.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochem Biophys 1979; 582: 67–78.

Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cell, tissue and fluids. In Methods In Enzymology. Edited by McCormick DB, Wright LD. New York: Academic; 1973: 1–11.

Hogberg J, Larson RE, Kristoferson A, Orrhenices IS. NADPH dependent reductase solublised from microsome by peroxidation and its activity. Biochem Biophys Res Commun 1974; 56: 836–842.

Devasagayam TPA , Tarachan U. Decreased lipid peroxidation in the rat kidney during gestation. Bichem Biophys Res Commun 1987; 56: 836–842.

Katewa SS, Chaudhary BL, Jain A. Folk herbal medicines from tribal areas of Rajasthan. India. J. Ethnopharmacol 2004; 92: 41-46.

Frode TS and Medeiros YS. Animal models to test drugs with potential anti diabetic activity. Journal of Ethanopharmacology 2008; 115: 173-183.

Bandawane DD, Bibave KH, Jaydeokar AV, Patil US, Hivrale MG. Antihyperglycemic and Antihyperlipidemic Effects of Methanolic Extract of Holarrhena antidysenterica Bark in Alloxan Induced Diabetes Mellitus in Rats. Pharmacologia 2013; 4(2): 95-106.

Amrani FE, Rhallab A , Alaoui T, Badaoui K, Chakir S. Hypoglycemic effects of Thymelea Hirsute in normal and streptozotocin induced diabetic rats. Journal of medicinal plants Research 2009; 3: 625-629.

Annie S, Rajendran K. Effect of aqueous bark extract of Garuga pinnata Roxb in streptozotocin nicotinamide induced Type 2 diabetes mellitus. J.Ethnopharmacol 2006; 107: 285-290.

Bettridge J. Lipid disorders in diabetes mellitus .Text book of diabetes Blackwell science London 2002; 45: 551-553.

Leontowicz H, Gorinstein S, Lojek A, Leontowicz M, Ciz M, Jung ST, et al. Comparative content of some bioactive compounds in apples, peaches and pears and their influence on lipids and antioxidant capacity in rats. J. Nutr. Biochem 2002; 13: 603-610.

Kaplowitz N (2001). Drug-induced liver disorders: Implications for drug development and regulation. Drug Safety 2001; 24: 483-490.

Verma N, Khosa RL. Hepatoprotective activity of leaves of Zanthoxylum armatum DC in Carbon tetra chloride induced hepatotoxicity in rats. Indian J Biochem Biophy.2010; 47: 124-127.

Jaspreet V, Sivakami S, Shahani S, Suthar AC, Banaralikar MM, Biyani MK. Antihyperglycemic effect of three extract from Monordica charantia. J Ethnopharmacol 2000; 88: 107–111.

Punitha ISR, Rajendran K, Shirwaikar A, Shirwaikar A. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocinnicotinamide induced diabetic rats. eCAM 2005; 2: 375–381.

Rajasekaran A, Kalaivani M. Antioxidant activity of aqueous extract of Monascus fermented Indian variety of rice in high cholesterol diet fed-Streptozotocin diabetic rats, an in vivo study. Int J Cur Sci Res 2011; 1(2): 35–38.

Nevin KG, Vijayammal PL. Effect of Aerva Lanata against carbon tetra chloride in rats. Experimental toxicology and pharmacology 2005; 20: 471-477.

Suryawanshi NP, Bhutey AK, Nagdeote AN, Jadhar AA, Manorkar GS. Study of lipid peroxide and lipid profile in diabetes mellitus. Indian journal of clinical Biochemistry 2006; 21: 126-130.

Published

2015-01-01

How to Cite

Ms, S., P. S, H. S, and D. K. “IN VIVO ANTIDIABETIC POTENTIAL OF CYCLEA PELTATA IN STREPTOZOTOCIN‑INDUCED‑DIABETIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 1, Jan. 2015, pp. 103-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1336.

Issue

Section

Original Article(s)