INCIDENCE AND VIRULENCE TRAITS OF CANDIDA DUBLINIENSIS ISOLATED FROM CLINICALLY SUSPECTED PATIENTS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.13567Abstract
       Objective: Fungal infections are caused by Candida species are increasing mainly in immunocompromised patients. Among Candida species, Candida dubliniensis has recently increasing opportunistic pathogenic yeast. The present study was aimed to assess the incidence and virulence factors of C. dubliniensis isolated from urine samples of various hospitalized clinically suspected patients. Methods: Totally 1,406 urine samples were processed, among that 9(0.64%) were identified as C. dubliniensis by germ tube production, biochemical test (Candida identification kit), CHROM agar Candida differential medium and growth at 45°C. Virulence factors of the C. dubliniensis viz., phospholipase, proteinase, esterase, lipase, haemolysin and biofilm production were detected by standard procedures. Results: Maximum number of C.dubliniensis have ability to produce protienase 6(66.66%), followed by esterase 5(55.55%), biofilm formation 4(44.44%), haemolysin 2(22.22%) and phospholipase, lipase 1(11.1%) respectively. Conclusion: The result revealed that these enzymes are potential virulence factors are the most important thing to realize pathogenesis of C. dubliniensis.
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Sullivan D, Haynes K, Bille J, Boerlin P, Rodero L et al. Widespread geographic distribution of oral Candida dubliniensis strains in human immunodeficiency virus infected individuals. J Clin Microbiol 1998;36:3007-3012.
Sullivan DJ, Moran GP, Coleman DC. Candida dubliniensis: Ten years on. FEMS Microbiol Let 2005;253:9-17.
Rizvi MW, Malik A, Shahid M, Singhal S. Candida albicans infections in a north Indian tertiary care hospital: Antifungal resistance pattern and role of SDS-PAGE for characterization. Biol and Med 201l;3(2):176-181.
Schoofs A, Odds FC, Colebunders R, Ieven M, Goossens H. Recognition and identification of Candida dubliniensis isolates from HIV patients: use of specialized isolation media. Eur J Clin Microbiol Infect Dis 1997;16:296-300.
Pinjon E, Sullivan D, Salkin I, Shanley D, Coleman D. Simple, inexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicans. J Clin Microbiol 1998;36:2093-2095.
Linares CEB, Loreto ES, Silveira CP, Pozzatti P, Scheid LA, Santurio JM, Alves SH. Enzymatic and haemolytic activities of Candida dubliniensis strains. Rev Inst Med trop Med S Paulo 2007;49(4):203-206,.
Schaller M, Borelli C, Korting HC, Hube B. Hydrolytic enzymes as virulence factors of Candida albicans. Mycos 2005;48:365-377.
Naglik J, Albrecht A, Bader O, Hube B. Candida albicans proteinases and host/pathogen interactions. Cell Microbiol 2004;6:915-926.
Perea S, Patterson TF. Antifungal resistance in pathogenic fungi. Clin Infect Dis 2002;35:1073-1080.
Semiha Ozkan, Fatma Kaynak, Ayse Kalkanci, Ufuk Abbasoglu, Semra Kustimur. Slime production and proteinase activity of Candida species isolated from blood samples and the comparison of these activities with minimum inhibitory concentration values of antifungal agents. Mem Inst Oswaldo Cruz Rio de Janeiro 2005;100(3):319-324.
Vijaya D, Harsha TR, Nagaratnamma T. Candida Speciation Using Chrom Agar. Journal of Clin and Diagnos Res 2011;5(4):755-757.
Samaranayake LP, Raeside JM, MacFarlane TW. Factors affecting the phospholipase activity of Candida species in vitro. Sabouraud 1984; 22:201-207.
Staib F. serum proteins as nitrogen source for yeast like fungi. Sabouraud 1965;4:187-193.
Aneeja KR. Experiments in microbiology, plant pathology, tissue culture and mushroom cultivation. II edn. Delhi: New Age International Publishers; 1996.
Slifkin M. Tween 80 opacity test responses of various Candida species. J Clin Microbiol. 2000;38(12):4626–8.
Branchini ML, Pfaller MA, Rhine- chalk berg J, Frempong T, Isenberg HD. Genotype variation and slime production among blood and catheter isolates of C. parapsilosis. J Clin Microbiol 1994;32:452–6.
Manns JM, Mosser DM, Buckley HR. Production of haemolytic factor by Candida albicans. Infect Immun 1994;62:5154-5156.
Price MF, Wilkinson ID, Gentry LO. Plate methods for detection of phospholipase activity in Candida albicans. Sabouraud 1982;20:7-14.
Ge YP, Lu GX, Shen YN, Liu WD. In vitro evaluation of phospholipase, proteinase, and esterase activities of Candida parapsilosis and Candida metapsilosis. Mycopathol 2011;172(6):429-38.
Sullivan D, Coleman D. Candida dubliniensis: characteristics and identification. J Clin Microbiol 1998;36:329-334.
Sullivan D, Haynes K, Bille J, Boerlin P, Rodero L, Lloyd S et al. Widespread geographic distribution of oral Candida dubliniensis strains in human immunodeficiency virus-infected individuals. J Clin Microbiol 1997;35:960–964.
Willis AM, Coulter WA, Sullivan DJ, Coleman DC, Hayes JR, Bell PM, Lamey PJ. Isolation of C. dubliniensis from insulin-using diabetes mellitus patients. J Oral Pathol Med 2000;29:86.
Pinjon E, Moran GP, Coleman DC, Sullivan DJ. Azole susceptibility and resistance in Candida dubliniensis. Biochem Soc Trans 2005;33:1210.
Ponton J, Ruchel R, Clemons KV, Coleman DC, Grillot R, Guarro J et al. Emerging pathogens. Med Mycol 2000;38 Suppl. 1:225-236.
Sullivan DJ, Westerneng TJ, Haynes KA, Bennet DE, Coleman DC. Candida dubliniensis sp. nov., phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiol 1995;141: 1507–1521.
Odds FC, Nuffel LV, Dams G. 1998. Prevalence of Candida dubliniensis isolates in a yeast stock collection. J Clin Microbiol 1998;36:2869–2873.
Quindós G, Carrillo-Muñoz AJ, Arévalo MP, Salgado J, Alonso-Vargas R, Rodrigo JM. et al. In vitro susceptibility of Candida dubliniensis to current and new antifungal agents. Chemother 2000;46:395–401.
Uma Chaudhary, Antariksh Deep and Narender Chabbra. Rapid identification and antifungal susceptibility pattern of Candida isolates. Infect Dis Antimicrob Agents 2009;26(2):49-53.
Gutiérrez J, Morales P, González MA and Quindós G. Candida dubliniensis, a new fungal pathogen. J Basic Microbiol 2002;42(3):207–227.
Stokes C, Moran GP, Spiering MJ, Cole GT, Coleman DC, Sullivan DJ. Lower filamentation rates of Candida dubliniensis contribute to its lower virulence in comparision with Candida albicans. Fungal Genet Biol 2007;22: In print.
Bennet DE. Genetic characterization of phospholipase C gene from Candida albicans, presence of Homologous sequences in Candida species other than Candida albicans. Microbiol 1998;144:55–72.
Sachin CD, Ruchi K, Santosh S. In vitro evaluation of proteinase, phospholipase and haemolysin activities of Candida species isolated from clinical specimens. Int J Med Biomed Res 2012;1(2):153-157.
Hannula J, Saarela M, Dogan B, Paatsama J, Koukila-Kahkola P, Pirinen S. et al. Comparison of virulence factors of oral Candida dubliniensis and Candida albicans isolates in Healthy people and patients with chronic candidosis. Oral Microbiol Immunol 2000;15:238-244.
Susanne Wilhelm, Jan Tommassen and Karl-Erich Jaeger. A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. J of Bacteriol 1999;181(22):6977-6986.
Rudek W. Esterase activity in Candida species J Clin Microbiol 1978;8:756-759.
Casal M, Linares MJ. Contribution to the study of the enzymatic profiles of yeast organisms with medical interest. Mycopathol 1983;81:155-159.
Pospisil L, Kabatova A. Lipolytic activity in some Candida strains. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg 1976;131:692-696.
Khosravi AR, Riazipour M, Shokri H, Mousavi M. Intracellular esterase activity of Candida albicans and its correlation with pathogenicity in mice. J Med Mycol 2008;18(3):134-40.
Chethrapilly Purushothaman Grish Kumar, Thangam Menon, Thangavel Sundararajan, Srathy Nalini, Mandayam Anandampillai Thiirunarayan, Sikhamani Rajasekaran and Manjetti Venkatadesikalu. Esterase activity of Candida species isolated from immunocompromised hosts. Rev Iberoam Micol 2006;23:101-103.
Martin S, Borelli C, Korting HC, Hube B. Hydrolytic enzymes as virulence factors of Candida albicans. Mycos 2005;48:365-37719.
Bier M. Lipases. Methods Enzymol 1955;1:627-642.
Nachimuthu Ramesh, Maruthupandian Priyadharsini, Chettipalayam Samiappan Sumathi, Velramar Balasubramanian, Janarthanam Hemapriya, Rajesh kannan. Virulence Factors and Anti Fungal Sensitivity Pattern of Candida sp. Isolated from HIV and TB Patients. Indian J Microbiol 2011;51(3):273-278.
Belanger M, Begin C, Jacques M. Lipopolysaccharides of Actino bacilluspleuropneumonia bind pig hemoglobin. Infect Immun 1995;63:656-662.
Boreck´a-Melkusov´a S, Moran GP, Sullivan DJ, Kuchar´ıkov´a S, Chorv´at D Jr., Bujd´akov´a H. The expression of genes involved in the ergosterol biosynthesis pathway in Candida albicans and Candida dubliniensis biofilms exposed to fluconazole,†Mycos 2009;52(2):118-128.
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