EVALUATION AND OPTIMIZATION OF ANTIOXIDANT POTENTIALITY OF XYLARIA FEEJEENSIS HMJAU22039
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s2.13734Abstract
ABSTRACT
Objective: Antioxidants neutralize free radicals generated in the human body and prevent them from causing damage. The present study is aimed to
evaluate and optimize the antioxidant activity of Xylaria feejeensis HMJAU22039 an endophyte isolated from Tectona grandis.
Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay and nitric oxide (NO) scavenging
assay. Total phenolic content (TPC) of the samples was measured by Folin–Ciocalteu reagent based assay, and the values were obtained from the
regression equation: y = 0.006141x + 0.157733.
Results: Potato dextrose yeast extract broth medium was selected as the basal medium as it reported a high antioxidant activity. The basal medium
was optimized with 2% dextrose, 0.2% yeast extract, and 200 g/L potato infusion. Dextrose and yeast extract were selected as carbon and nitrogen
sources, respectively, as they reported high antioxidant activity. The antioxidant activity and phenolic content are highest at static condition
(18.14 mg/g). Incubation temperature of 30°C and pH of the initial medium at 6 were found to be optimum conditions for high antioxidant activity. An
incubation period of 20 days reported the highest antioxidant activity and phenolic content. Methanol extract recorded high antioxidant activity with
a DPPH (73.86%) followed by ethyl acetate and chloroform fractions.
Conclusion: The study highlights the importance of different physiochemical parameters in the production of secondary metabolites having
antioxidant properties. The results reveal a significant positive correlation between DPPH radical scavenging assay, NO scavenging assay, and TPC.
Keywords: 1-1diphenyl-2-picryl-hydrazil, Total phenolic content, Endophytic fungus, Tectona grandis.
Downloads
References
REFERENCES
Tan RX, Zou WX. Endophytes: A rich source of functional metabolites.
Nat Prod Rep 2001;18(4):448-59.
Gunatilaka AA. Natural products from plant-associated microorganisms:
Distribution, structural diversity, bioactivity and implication of their
occurrence. J Nat Prod 2006;69(3):509-26.
Ruma K, Sunil K, Prakash HS. Antioxidant, anti-inflammatory,
antimicrobial and cytotoxic properties of fungal endophytes from
Garcinia species. Int J Pharm Pharm Sci 2013;5(3):889-97.
Prabukumar S, Rajkuberan C, Ravindran K, Sivaramakrishnan S.
Isolation and characterization of endophytic fungi from medicinal plant
Crescentia cujete L. And their antibacterial, antioxidant and anticancer
properties. Int J Pharm Pharm Sci 2015;7(11):316-21.
Gursoy N, Sarikurkcu C, Cengiz M, Solak MH. Antioxidant activities,
metal contents, total phenolics and flavonoids of seven Morchella
species. Food Chem Toxicol 2009;47(9):2381-8.
Rebbapragada and Kalyanaraman
Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 269-273
Rodrigues KF, Costa GL, Carvalho MP, Epifanio RA. Evaluation of
extracts produced by some tropical fungi as potential cholinesterase
inhibitors. World J Microbiol Biotechnol 2005;21:1617-21.
Song YC, Huang WY, Sun C, Wang FW, Tan RX. Characterization of
graphislactone a as the antioxidant and free radical-scavenging substance
from the culture of Cephalosporium sp. IFB-E001, an endophytic fungus
in Trachelospermum jasminoides. Biol Pharm Bull 2005;28(3):506-9.
Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX. Antioxidant activities of
Salvia miltiorrhiza and Panax notoginseng. Food Chem 2006;99:767-74.
Kang KS, Yokozawa T, Kim HY, Park JH. Study on the nitric oxide
scavenging effects of ginseng and its compounds. J Agric Food Chem
;54(7):2558-62.
Singleton VL, Ortofehr R, Lamuela-Raventos RM. Analysis of total
phenols and other oxidation substrate and antioxidants by means of
Folin–Ciocalteau reagent. Methods Enzymol 1999;299:152-78.
Harper JK, Arif AM, Ford EJ, Strobel GA, Porco JA, Tomer DP, et al.
Pestacin: A 1,3 dihydro isobenzofuran from Pestalotiopsis microspora
possessing antioxidant and antimycotic activities. Tetrahedron
;59:2471-76.
Strobel G, Ford E, Worapong J, Harper JK, Arif AM, Grant DM, et al.
Isopestacin, an isobenzofuranone from Pestalotiopsis microspora,
possessing antifungal and antioxidant activities. Phytochemistry
;60(2):179-83.
Liu X, Dong M, Chen X, Jiang M, Lv X, Yan G. Antioxidant activity
and phenolics of an endophytic Xylaria sp. From Ginkgo biloba. Food
Chem 2007;105(2):554-84.
Thakur D, Bora TC, Bordoloi GN, Maiumdar S. Influence of nutrition
and culturing conditions for optimum growth and antimicrobial
metabolite production by Streptomyces sp.201. J Med Mycol
;19:161-7.
Agastian P, Merlin JN, Nimal Christhudas IV, Praveen Kumar P.
Optimization of growth and bioactive metabolite production: Fusarium
solani. Asian J Pharm Clin Res 2013;6(3):98-103.
Ramesh V, Karunakaran C, Rajendran A. Optimization of submerged
culture conditions for mycelial biomass production with enhanced
antibacterial activity of the medicinal macro fungus Xylaria sp. Strain
R006 against drug resistant bacterial pathogens. Curr Res Environ Appl
Mycol 2014;4(1):88-98.
Arora DS, Chandra P, Kau GJ. Optimization and assay of antioxidant
potential of two Penicillium spp. By different procedures. Curr
Biotechnol 2012;1(1):2-10.
Abo-Elmagd HI. Evaluation and optimization of antioxidant potentiality
of Chaetomium madrasense AUMC 9376. J Genet Eng Biotechnol
;12(1):21-6.
Bhattacharyya PN, Jha DK. Optimization of cultural conditions affecting
growth and improved bioactive metabolite production by a subsurface
Aspergillus strain TSF 146. Int J Appl Biol Pharm 2011;2(4):134-43.
Gazi MR, Kanda K, Kato F. Optimization of various cultural conditions
on growth and antioxidant activity aeneration by Saccharomyces
cerevisiae IFO 2373. J Biol Sci 2004;4(2):224-28.
Yamanaka T. The effect of pH on the growth of saprotrophic and
ectomycorrhizal ammonia fungi in vitro. Mycologia 2003;95(4):584-9.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
