â€œSTUDY OF TOTAL CHOLESTEROL (TC), TRIACYLGLYCEROLS (TG), HIGH DENSITY LIPOPROTEIN CHOLESTEROL (HDL-C) IN TYPE II DIABETES MELLITUS.â€
Objective: There are probably 100 million people in the world with diabetes mellitus (DM), and incidences of diabetes are on the rise. Dyslipidemia is one of the common disorders which are seen in most of the diabetes patients, which causes cardiovascular disorders. The aim of this study is to investigate the total cholesterol (TC), triacylglycerols (TGs), high-density lipoprotein cholesterol (HDL-C) in Type 2 DM, and healthy controls.
Methods: The study was conducted on 50 controls and 50 Type 2 diabetic subjects between age group of 30 and 60 years. Serum TC was determined by an enzymatic (cholesterol oxidase/phenol-aminophenazone [PAP]) colorimetric method and TGs were determined by an enzymatic (glycerol phosphate oxidase-PAP) method, and HDL-C was estimated by a precipitant method. Statistical analysis was done using unpaired t-test.
Results: The mean value of TC, TGs, and HDL-C in normal subjects is 165.5Â±24.24, 118.7Â±41.58, 28.38Â±7.85 mg/dl, respectively, and the value of TC, TGs, and HDL-C in diabetic patients is 179Â±31.69, 164.35Â±27.93, 25.4Â±6.86 mg/dl, respectively. The observed difference in the means of TC, TGs, and HDL-C in normal and diabetic are statistically significant (p<0.05).
Conclusion: From the present study, it is concluded that TC, TGs, and HDL-C levels for all persons with Type 2 DM should be done as a routine test. Furthermore, early diagnosis and treatment of dyslipidemia can be used as a preventive measure for the development of cardiovascular disease in Type 2 diabetes.
Keywords: Diabetes mellitus, Dyslipidemia, Lipid profile, Total cholesterol, Triglycerides, High-density lipoprotein cholesterol.
Minor alterations in thyroid-function tests associated with diabetes mellitus and obesity in outpatients without known thyroid illness. Acta Clin Belg 2001;56(2):86-90.
2. Unwin N, Sobngwi E, Alberti KG. Type 2 diabetes: The challenge of preventing a global epidemic. Diabetes Int 2001;11:4-8.
3. Allain CC, Poon IS, Chan CH, Richmond W. Enzymatic determination of serum total cholesterol. Clin Chem 1974;20(4):470-1.
4. Jacobs NJ, Van Denmark PJ. Enzymatic determination of serum
triglycerides. Biochem Biophys 1960;88:250-5.
5. Gordon T, Gordon M. An enzymatic method for the determination of the serum HDL-cholesterol. Am J Med 1977;62:707-8.
6. Kreisberg RA. Diabetic dyslipidemia. Am J Cardiol
7. Kesavuler MM, Giri R, Rao BK, Apparao C. Lipid peroxidation and antioxidant enzyme levels in Type II diabetics with micro vascular complications. Diabetes Metab 2000;26(5):387-92.
8. Idogun ES, Unuigbe EI, Ogunro PS, Akinola OT, Famodu AA.
Assessment of the serum lipids in Nigerians with Type 2 diabetes mellitus complications. Pak J Med Sci (Part 1) 2007;23(5):708-12.
9. Albrki WM, Elzouki AN, EL-Mansoury ZM, Tashani OA. Lipid profiles in Libian Type 2 diabetes. J Sci Appl 2007;1(1):18-23.
10. Harder H, Dinesen B, Astrup A. The effect of a rapid weight loss on lipid profile and glycemic control in obese Type 2 diabetic patients. Int J Obes Relat Metab Disord 2004;28(1):180-2.
11. Gordon L, Ragoobirsingh D, Morrison EY, Choo-Kang E, McGrowder D,
Martorell E. Lipid profile of Type 2 diabetic and hypertensive patients in the Jamaican population. J Lab Physicians 2010;2(1):25-30.
12. Vikram NK, Tandon N, Misra A, Srivastava MC, Pandey RM, Mithal A, et al. Correlates of Type II diabetes mellitus in children, adolescents and young adults in North India: A multisite collaborative case control study. Diabet Med 2006;23(3):293-8.
13. Fielding CJ, Reaven GM, Fielding PE. Human noninsulin-dependent diabetes: Identification of a defect in plasma cholesterol transport normalized in vivo by insulin and in vitro by selective immunoadsorption of apolipoprotein E. Proc Natl Acad Sci U S A 1982;79:6365-9.
14. DeFronzo RA. Lilly lecture 1987. The triumvirate: Beta-cell, muscle, liver. A collusion responsible for NIDDM. Diabetes 1988;37(6):667-87.
15. Chatterjee MN, Shinde R. Metabolism of carbohydrates. 6th ed.
New Delhi, India: Jaypee Brothers Medical Publisher; 2005. p. 266-330.
16. Del Prato S, Bonadonna RC, Bosom E, Gulli G, Solini A, Shank M, et al.
Characterization cellular defects of insulin action in Type2 (noninsulin dependent) diabetes mellitus. J Clin Invest 1993;91(2):484-94.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.