ANTIDIABETIC ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM FICUS RELIGIOSA


Palak Tiwari, Nathiya R, Gayathri Mahalingam

Abstract


Objective- The aim was to study in vitro anti diabetic activity of endophytic fungi isolated from Ficus Religiosa.

Method- The explants (leaves and stem) were processed on the potato dextrose media nine colonies was found and colony frequency was calculated. All the colonies were transferred onto potato dextrose broth and incubated for 21 days. The crude was extracted using three solvents petroleum ether (0.1), diethyl ether (2.8) and ethyl acetate (4.4). Three assays were performed to determine in vitro anti diabetic activity of crude extract (α-amylase inhibition assay, α-glucosidase inhibition assay and glucose diffusion assay) and the % of inhibition by crude and standard acarbose was calculated with standard error mean.

Results- The endophytic fungi shows the highest % of inhibition for α-amylase inhibition assay (91 % ± 0.06), α-glucosidase inhibition assay (42% ± 0.01).

Conclusion-The results indicates that the hypoglycemic activity of the endophytic crude extract has been proved, hence further studies are focused on to isolate and purify the bioactive compounds and test for in vivo animal studies to confirm the anti diabetic activity.

Keywords: Endophytic fungi, Antidiabetic activity, α-amylase, α-glucosidase.


| PDF |

References


Rajagopal K, Sundharamoorthy M, Arumugam P, Basha WJ, Govindarajan K, Rajendran R. In vitro anti bacterial activity of endophytic fungal extracts isolated from a pharmaceutically important plant Ficus religiosa L. Int J Pharm Bio Sci 2015;6(4):1093-8.

Azevedo JL, Maccheroni JW, Pereira JO. Endophytic microorganisms: A review on insect control and recent advances on tropical plants. Electron J Biotechnol 2000;3(1):40-65.

Subashini D, Subhashree V. Antioxidant and α-amylase inhibition activities of phenolic compounds in the extracts of Indian honey. Chin J Nat Med 2012;10(4):255-9.

Lakshmi PJ, Selvi KV. Anticancer potentials of secondary metabolites from endophytes of Barringtonia acutangula and its molecular characterization. Int J Curr Microbiol Appl Sci 2013;2(2):44-5.

Onifade AK. Research trends: Bioactive metabolites of fungal origin. Res J Biol Sci 2007;2:81-4.

Picot C, Subratty AH, Mahomoodally MF. Inhibitory potential of five traditionally used native antidiabetic medicinal plants on α-amylase, α-glucosidase, glucose entrapment, and amylolysis kinetics in vitro. Adv Pharmacol Sci 2014;2014:1-7.

Bouché C, Serdy S, Kahn CR, Goldfine AB. The cellular fate of glucose and its relevance in Type 2 diabetes. Endocr Rev 2004;25(5):807-30.

Strobel G, Daisy B, Castillo U. The biological promise of microbial endophytes and their natural products. Plant Pathol J 2005;4(2):161-76.

Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81(1):81-100.

Janecek S, Baláz S. Alpha-amylases and approaches leading to their enhanced stability. FEBS Lett 1992;304(1):1-3.

Muus J, Vnenchak JM. Isozymes of salivary amylase. Nature 1964;204:283-5.

Prabhavathy D, Nachiyar V. Antimicrobial and antidiabetic activity of an endophytic fungi isolated from Adathoda beddomei. Int J Pharm Pharm Sci 2013;5(3):780-3.

Chandrasekar SB, Bhanumathy M, Pawar AT, Somasundaram T. Phytopharmacology of Ficus religiosa. Pharmacogn Rev 2010;4(8):195-9.

Singh D, Singh B, Goel RK. Traditional uses, phytochemistry and pharmacology of Ficus religiosa: A review. J Ethnopharmacol 2011;134(3):565-83.

Tan RX, Zou WX. Endophytes: A rich source of functional metabolites. Nat Prod Rep 2001;18(4):448-59.




About this article

Title

ANTIDIABETIC ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM FICUS RELIGIOSA

DOI

10.22159/ajpcr.2017.v10i4.14718

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 59-61

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

252 Views | 185 Downloads

Authors & Affiliations

Palak Tiwari
Vellore Institute of Technology

Nathiya R

Gayathri Mahalingam


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.