STUDIES ON BIOACTIVE POTENTIAL OF STREPTOMYCES SPP. (KX710212). ISOLATED FROM ENVIRONMENTAL POLLUTED SAMPLE, RANIPET, VELLORE, INDIA
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s3.14788Abstract
ABSTRACT
Objective: Natural products from bacterial origin played and still play a valuable role in drug discovery and provide diverse health-promoting
compounds that help to cure or alleviate serious diseases.
Methods: A total of 4 actinobacterial strains were isolated from 2 different environmental polluted soil samples collected from Ranipet, Vellore, Tamil
Nadu, India. Of 15 isolates screened for antibacterial property, 4 isolates (PS1, PS2, and PS3) were found potent and characterized phenotypically and
genotypically.
Results: The 16S rDNA gene sequences of potent strain showed 97% similarity with Streptomyces spp. and termed as Streptomyces sp. The bioactivity
was revealed by antibacterial and antioxidant activity.
Conclusion: Hence, these findings provide scientific evidence to validate the pharmacological use and have the potential contribution in the discovery
of new lead that play a key role in the field of therapeutics.
Keywords: Actinomycetes, Bioactive compounds, Antibacterial property.
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References
Choudury A, RahmanAbdur AA, Gafur AG. In vitro antibacterial and cytotoxic activities of a brown antibiotic metabolite from a strain of Actinomycetes. Science 2001;1(4):206-8.
Boudjella H, Bouti K, Zitouni A, Mathieu F, Lebrihi A, Sabaou N. Isolation and partial characterization of pigment-like antibiotics produced by a new strain of Streptosporangium isolated from an Algerian soil. J Appl Microbiol 2007;103(1):228-36.
Chater KF. Regulation of sporulation in Streptomyces coelicolor A3(2): A checkpoint multiplex? Curr Opin Microbiol 2001;4(6):667-73.
Hopwood DA. Forty years of genetics with Streptomyces: From in vivo through in vitro to in silico. Microbiology 1999;145 (Pt 9):2183-202.
Laksmanaperumalsamy P, Chandramohan D, Natarajan R. Antibacterial and antifungal activity of Streptomycetes from Porto Novo coastal environment. Mar Biol 1978;11:15-24.
Maniatis T, Tritsch EF, Sambrook, J. Molecular cloning. In: A Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor; 1989.
Sivasubramanian R. Brindha P. In vitro cytotoxic, antioxidant and GC-MS Studies on Centratherum punctatum. Int J Pharm Pharm Sci 2013;5(3):364-7.
Ilić SB, Sandra S. Konstantinović ZB. UV/VIS analysis and antimicrobial activity of Streptomyces isolates. Facta Univ Ser Med Boil 2005;2(1):44-6.
Wang Y. Zhang ZS. Ruan JS. Wang YM, Ali SM. Investigation of actinomycete diversity in the tropical rainforests of Singapore. J Ind Microbiol 1999;23:178-87.
Warth AD. Mechanism of action of benzoic acid on Zygosaccharomyces bailii: Effects on glycolytic metabolite levels, energy production, and intracellular pH. Appl Environ Microbiol 1991;57(12):3410-4.
Williams S.T., Cross T. Isolation, purification, cultivation and preservation of actinomycetes. Methods Microbiol 1971;4:295-333.
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