OBTENTION OF PROTEIN CONCENTRATE AND POLYPHENOLS FROM MACADAMIA (MACADAMIA INTEGRIFOLIA) WITH AQUEOUS EXTRACTION METHOD

  • Wilman Carrillo Universidad Técnica de Ambato http://orcid.org/0000-0002-7609-2939
  • David Lara Universidad Técnica de Ambato
  • Edgar Vilcacundo Universidad Estatal de Bolivar
  • Cristian Carrillo Universidad Técnica de Ambato
  • Monica Silva Universidad Técnica de Ambato
  • Mario Alvarez Universidad Técnica de Ambato
  • Cecilia Carpio Universidad Técnica de Ambato

Abstract

Objective: The aim of this study was to obtain protein concentrates from macadamia using alkaline pH at different pHs of precipitation with water to analyze the protein isolates using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Materials & Methods: Macadamia protein concentrates were obtained using the isoelectric precipitation method at different pHs using water as solvent. Proteins were analyzed using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Results: A yield of 36.57±0.17a of protein concentrate of defatted macadamia flour at pH 6.0 with a 51.564% of protein was obtained using the Dumas method. Polypeptides profile was identified in the 11-63 kDa range. Total polyphenols content was high at pH 5.0 with a value of 367,340 mg GAE equivalents / 100 g.

Conclusions: Macadamia seed is a good source of proteins. Native-PAGE, SDS-PAGE and RP-UHPLC are good methods to identify the macadamia protein isolate in presence of water.  

Author Biographies

Wilman Carrillo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeería en Aliementos
David Lara, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeniería en Alimentos
Edgar Vilcacundo, Universidad Estatal de Bolivar
Facultad de Ciencias Agropecuarias
Cristian Carrillo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeniería en Alimentos
Monica Silva, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeniería en Alimentos
Mario Alvarez, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeniería en Alimentos
Cecilia Carpio, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeniería en Alimentos

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How to Cite
Carrillo, W., D. Lara, E. Vilcacundo, C. Carrillo, M. Silva, M. Alvarez, and C. Carpio. “OBTENTION OF PROTEIN CONCENTRATE AND POLYPHENOLS FROM MACADAMIA (MACADAMIA INTEGRIFOLIA) WITH AQUEOUS EXTRACTION METHOD”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 138-42, doi:10.22159/ajpcr.2017.v10i2.14808.
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