• LARA D Universidad Técnica de Ambato
  • VILCACUNDO E Universidad Estatal de Bolivar
  • CARRILLO C Universidad Técnica de Ambato
  • CARPIO C Universidad Técnica de Ambato
  • SILVA M Universidad Técnica de Ambato
  • ALVAREZ M Universidad Técnica de Ambato
  • CARRILLO W Universidad Técnica de Ambato http://orcid.org/0000-0002-7609-2939




Objective: The aim of this study was to obtain protein concentrates from macadamia using alkaline pH at different pHs of precipitation with water to analyze the protein isolates using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Materials & Methods: Macadamia protein concentrates were obtained using the isoelectric precipitation method at different pHs using water as solvent. Proteins were analyzed using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Results: A yield of 36.57±0.17a of protein concentrate of defatted macadamia flour at pH 6.0 with a 51.564% of protein was obtained using the Dumas method. Polypeptides profile was identified in the 11-63 kDa range. Total polyphenols content was high at pH 5.0 with a value of 367,340 mg GAE equivalents / 100 g.

Conclusions: Macadamia seed is a good source of proteins. Native-PAGE, SDS-PAGE and RP-UHPLC are good methods to identify the macadamia protein isolate in presence of water.  


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Author Biographies

LARA D, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeniería en Alimentos

VILCACUNDO E, Universidad Estatal de Bolivar

Facultad de Ciencias Agropecuarias

CARRILLO C, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeniería en Alimentos

CARPIO C, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeniería en Alimentos

SILVA M, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeniería en Alimentos

ALVAREZ M, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeniería en Alimentos

CARRILLO W, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeería en Aliementos


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How to Cite

LARA D, VILCACUNDO E, CARRILLO C, CARPIO C, SILVA M, ALVAREZ M, and CARRILLO W. “OBTENTION OF PROTEIN CONCENTRATE AND POLYPHENOLS FROM MACADAMIA (MACADAMIA INTEGRIFOLIA) WITH AQUEOUS EXTRACTION METHOD”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 2, Feb. 2017, pp. 138-42, doi:10.22159/ajpcr.2017.v10i2.14808.



Original Article(s)