Wilman Carrillo, David Lara, Edgar Vilcacundo, Cristian Carrillo, Monica Silva, Mario Alvarez, Cecilia Carpio


Objective: The aim of this study was to obtain protein concentrates from macadamia using alkaline pH at different pHs of precipitation with water to analyze the protein isolates using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Materials & Methods: Macadamia protein concentrates were obtained using the isoelectric precipitation method at different pHs using water as solvent. Proteins were analyzed using the Native-PAGE, SDS-PAGE electrophoresis and RP-UHPLC methods.

Results: A yield of 36.57±0.17a of protein concentrate of defatted macadamia flour at pH 6.0 with a 51.564% of protein was obtained using the Dumas method. Polypeptides profile was identified in the 11-63 kDa range. Total polyphenols content was high at pH 5.0 with a value of 367,340 mg GAE equivalents / 100 g.

Conclusions: Macadamia seed is a good source of proteins. Native-PAGE, SDS-PAGE and RP-UHPLC are good methods to identify the macadamia protein isolate in presence of water.  

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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 2 February 2017 Page: 138-142

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Authors & Affiliations

Wilman Carrillo
Universidad Técnica de Ambato

David Lara
Universidad Técnica de Ambato

Edgar Vilcacundo
Universidad Estatal de Bolivar

Cristian Carrillo
Universidad Técnica de Ambato

Monica Silva
Universidad Técnica de Ambato

Mario Alvarez
Universidad Técnica de Ambato

Cecilia Carpio
Universidad Técnica de Ambato

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