BSMI AND TAQI POLYMORPHISMS IN VITAMIN D RECEPTOR GENE OF TYPE 2 DIABETES MELLITUS PATIENTS FROM NORTH INDIA
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s3.14875Abstract
ABSTRACT
Objective: Polymorphisms in vitamin D receptor (VDR) genes are known to be linked with different metabolic diseases including Type 2 diabetes
mellitus (T2DM) also. However, the association of these polymorphisms is not much explored for the Indian population. To determine the prevalence
of BsmI and TaqI polymorphism in VDR gene of T2DM patients from North India.
Methods: Blood samples were obtained from 100 well-characterized T2DM patients and 100 healthy controls. Genomic DNA was isolated from blood
samples and using polymerase chain reaction/restriction fragment length polymorphism based method, the presence of these polymorphisms was
investigated in these samples. The data were statistically analyzed using SPSS 21.0 software.
Results: For TaqI polymorphism, both the wild type (TT) and heterozygous (TC) genotype showed a significant difference between patients and
controls (p=0.023 and p<0.001, respectively). Whereas, the frequency of CC genotype was not significantly different among these groups (p=0.506).
For BsmI polymorphism also, the frequency of wild type (GG) and heterozygous (GA) genotype was significantly different in patients and controls
(p=0.027 and p=0.001), respectively. However, the frequency of AA genotype was not of statistical significance in patients (p=0.071).
Conclusions: The mutant alleles of TaqI and BsmI polymorphisms are known to be associated with different metabolic diseases, including diabetes
too. In our study also, there is a significant difference between the frequency of wild type and heterozygous genotype for these polymorphisms. This
suggests that BsmI and TaqI polymorphisms may be associated with T2DM patients.
Keywords: Type 2 diabetes mellitus, Polymorphism, Vitamin D receptor, Patient, Control, Restriction fragment length polymorphism.
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