OCCURRENCE OF CARBAPENEMASES AND EXTENDED-SPECTRUM Î’ETA-LACTAMASES IN UROPATHOGENIC ENTEROBACTERIACEAE ISOLATED FROM A COMMUNITY SETTING, SETTAT, MOROCCO

  • Samira Natoubi Agrofood and Health Laboratory, Faculty of Science and Technology, University Hassan I, 26000, Settat, Morocco.
  • Abouddihaj Barguigua
  • Nezha Baghdad
  • Kaotar Nayme
  • Mohammed Timinouni
  • Abderraouf Hilali
  • Souad Amghar
  • Khalid Zerouali

Abstract

Objective: Urinary tract infections (UTIs) are still commonly diagnosed in outpatients as well as in hospitalized patients. In this study, we
investigated the prevalence and performed molecular characterization of extended-spectrum-β-lactamases (ESBL) and carbapenemases produced by
Enterobacteriaceae isolates that cause community UTIs in Settat city, Morocco.

Methods: From January 2012 to December 2013, all uropathogenic community Enterobacteriaceae isolates were collected from the microbiology
laboratory of Hassan II Hospital, Settat, Morocco. Antibiotic susceptibility testing was performed as recommended by Clinical and Laboratory
Standard Institute. Phenotypic identification of ESBL and carbapenemase producer isolates was confirmed by the double-disk synergy test and the
modified Hodge test, respectively. Molecular characterization of β-lactamase genes was performed using polymerase chain reaction (PCR), followed
by sequencing of the obtained products.

Results: Among 153 isolates, 31 (20.26%) were multi-drug resistant (MDR). Nine strains (5.88%) were ESBL producers, of which Klebsiella
pneumoniae (n=5; 20.83%), Escherichia coli (n=3; 3.15%), and Enterobacter cloacae (n=1; 9.09%) species were identified. The results of ESBLencoding
gene detection by sequencing revealed the presence of CTX-M-15 (n=9) in association with other β-lactamase genes such as temoneira 1 (n=8) and sulfhydryl variable 1 (n=5). According to the modified Hodge test and PCR, three isolates (1.96%) were positive carry the bla

Conclusion: The emergence of MDR uropathogenic Enterobacteriaceae isolates in our community is highly alarming. Strict measures will be required to control the further spread of these uropathogenic isolates. Keywords: Carbapenemase, Enterobacteriaceae, Extended-spectrum-β-lactamases, Moroccan community.
OXA-48 gene.

Keywords: Carbapenemase, Enterobacteriaceae, Extended-spectrum-β-lactamases, Moroccan community.

Objective: Urinary tract infections (UTIs) are still commonly diagnosed in outpatients as well as in hospitalized patients. In this study, we investigatedtheprevalenceandperformedmolecularcharacterizationofextended-spectrum-β-lactamases(ESBL)andcarbapenemasesproducedby Enterobacteriaceae isolates that cause community UTIs in Settat city, Morocco.

 

Methods: From January 2012 to December 2013, all uropathogenic community Enterobacteriaceae isolates were collected from the microbiology laboratory of Hassan II Hospital, Settat, Morocco. Antibiotic susceptibility testing was performed as recommended by Clinical and Laboratory Standard Institute. Phenotypic identification of ESBL and carbapenemase producer isolates was confirmed by the double-disk synergy test and the modified Hodge test, respectively. Molecular characterization of β-lactamase genes was performed using polymerase chain reaction (PCR), followed by sequencing of the obtained products.

 

Results: Among 153 isolates, 31 (20.26%) were multi-drug resistant (MDR). Nine strains (5.88%) were ESBL producers, of which Klebsiella pneumoniae (n=5; 20.83%), Escherichia coli (n=3; 3.15%), and Enterobacter cloacae (n=1; 9.09%) species were identified. The results of ESBL- encodinggenedetectionbysequencingrevealedthepresenceofCTX-M-15(n=9)inassociationwithotherβ-lactamasegenessuchastemoneira1


OXA-48

 

(n=8) and sulfhydryl variable 1 (n=5). According to the modified Hodge test and PCR, three isolates (1.96%) were positive carry the bla


gene.


 

Conclusion:TheemergenceofMDRuropathogenicEnterobacteriaceaeisolatesinourcommunityishighlyalarming.Strictmeasureswillberequired

to control the further spread of these uropathogenic isolates.

 

Keywords: Carbapenemase, Enterobacteriaceae, Extended-spectrum-β-lactamases, Moroccan community.

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Natoubi, S., A. Barguigua, N. Baghdad, K. Nayme, M. Timinouni, A. Hilali, S. Amghar, and K. Zerouali. “OCCURRENCE OF CARBAPENEMASES AND EXTENDED-SPECTRUM Î’ETA-LACTAMASES IN UROPATHOGENIC ENTEROBACTERIACEAE ISOLATED FROM A COMMUNITY SETTING, SETTAT, MOROCCO”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 1, Jan. 2017, pp. 211-5, doi:10.22159/ajpcr.2017.v10i1.14924.
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