• Dayana Janakiraman Research Scholar,Department of Biochemistry, Bharathi Women's College, Chennai-108
  • Parameswari Somasundaram Principal (Retd), Government Arts college for women, Ramanadapuram


Objective: Plectranthus amboinicus, (PA) leaves contain pharmaceutically important phyto constituents that attribute to greater medicinal values. The purpose of the present study was to investigate the active principles present in the potent Hydroalcoholic extract of P.amboinicus and brine shrimp lethality studies to understand the toxicity profile of PA.

Methods: An approach towards High performance liquid chromatography (HPLC) screening of Hydroalcoholic extract of P.amboinicus and selective partitioning using various solvents such as Hexane, chloroform, ethylacetate and butanol were performed to identify the active principles present. Acute oral toxicity or brine shrimp lethality studies on Artemia salina L. using active fraction of PA were also attempted.

Results: The HPLC profile of ethylacetate fraction of Hydroalcoholic extract showed flavonoids such as Rutin, quercetin, luteolin and gallic acid, which was found to be considerably different from Hydroalcoholic extract and this fraction also showed good acute oral toxicity effect and the LC50 value obtained was 198.630µg/ml.

Conclusion: These results attribute to the pharmacological importance of the plant. Selective Isolation of individual phytochemical constituents and subjecting it to biological testing will definitely give promising results for medicinal field.

Author Biographies

Dayana Janakiraman, Research Scholar,Department of Biochemistry, Bharathi Women's College, Chennai-108
Department of Biochemistry
Parameswari Somasundaram, Principal (Retd), Government Arts college for women, Ramanadapuram


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How to Cite
Janakiraman, D., and P. Somasundaram. “ACTIVE PHENOLIC CONSTITUENTS AND BRINE SHRIMP LETHALITY ASSAY OF HYDROALCOHOLIC EXTRACT OF Plectranthus Amboinicus”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 186-9, doi:10.22159/ajpcr.2017.v10i2.15115.
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