ACTIVE PHENOLIC CONSTITUENTS AND BRINE SHRIMP LETHALITY ASSAY OF HYDROALCOHOLIC EXTRACT OF Plectranthus amboinicus

  • Dayana Janakiraman Research Scholar,Department of Biochemistry, Bharathi Women's College, Chennai-108
  • Parameswari Somasundaram Principal (Retd), Government Arts college for women, Ramanadapuram

Abstract

Objective: Plectranthus amboinicus, (PA) leaves contain pharmaceutically important phyto constituents that attribute to greater medicinal values. The purpose of the present study was to investigate the active principles present in the potent Hydroalcoholic extract of P.amboinicus and brine shrimp lethality studies to understand the toxicity profile of PA.

Methods: An approach towards High performance liquid chromatography (HPLC) screening of Hydroalcoholic extract of P.amboinicus and selective partitioning using various solvents such as Hexane, chloroform, ethylacetate and butanol were performed to identify the active principles present. Acute oral toxicity or brine shrimp lethality studies on Artemia salina L. using active fraction of PA were also attempted.

Results: The HPLC profile of ethylacetate fraction of Hydroalcoholic extract showed flavonoids such as Rutin, quercetin, luteolin and gallic acid, which was found to be considerably different from Hydroalcoholic extract and this fraction also showed good acute oral toxicity effect and the LC50 value obtained was 198.630µg/ml.

Conclusion: These results attribute to the pharmacological importance of the plant. Selective Isolation of individual phytochemical constituents and subjecting it to biological testing will definitely give promising results for medicinal field.

Author Biographies

Dayana Janakiraman, Research Scholar,Department of Biochemistry, Bharathi Women's College, Chennai-108
Department of Biochemistry
Parameswari Somasundaram, Principal (Retd), Government Arts college for women, Ramanadapuram
Biochemistry

References

1. Perl A. Pathogenesis and spectrum of autoimmunity. Methods Mol Med
2004;102:1-8.
2. Ramaswamy M, Deng M, Siegel RM. Harnessing programmed cell death as a therapeutic strategy in rheumatic diseases. Nat Rev

Rheumatol 2011;7(3):152-60.
3. Kirtikar KR, Basu BD. Indian Medicinal Plants. 3rd ed. Dehradun, India: International Book Distributors; 1999. p. 1970-1.
4. Warier PK, Nambier VP. Indian Medicinal Plants: A Compendium of
500 Species. 4th ed. Chennai: Orient Longman Limited; 1996. p. 315.
5. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants.
New Delhi, India: C.S.I.R. Publications; 1999. p. 74.
6. Chandrappa MS, Harsha R, Dinesh R, Gowda SS. Antibacterial activity of Coleus aromaticus leaves. Int J Pharm Pharm Sci 2010;2(3):63-6.
7. Manjamalai A, Narala Y, Haridas A, Berlin Grace VM. Antifungal, anti-inflammatory and GC-MS analysis of methanolic extract of Plectranthus amboinicus leaf. Int J Curr Pharm Res 2011;3(2):129-36.
8. Raman N. Phytochemical Techniques. New Delhi: New India
Publishing Agency; 2006. p. 19.
9. Harborne JB. Phytochemical Methods. New Delhi: Springer (India) Pvt. Ltd.; 2005. p. 17
10. Janakiraman D, Parameswari CS. Evaluation of anti-inflammatory effect of Plectranthus amboinicus leaf extract - An in vitro study. J Adv Pharm Educ Res 2014;4(2):229-32.
11. Dayana J, Parameswari CS. New perspective of stress combating potential of Plectranthus amboinicus (Lour) leaves - An in vitro study. Int J Pharm Res Biosci 2014;3(3):452-71.
12. Meyer N, Ferrigni NR, Putnam JE. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med 1982;45:31-2.
13. Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson D. A micro well cytotoxicity using Artemia salina (Brine shrimp). Planta Med
1992;59:250-2.
14. Apu AS, Bhuyan SH, Khatun, F, Liza MS, Matin M, Hossain F.
Assessment of cytotoxic activity of two medicinal plants using Brine shrimp (Artemia salina) as an experimental tool. Int J Pharm Sci Res
2013;4(3):1125-30.
15. Gupta SK, Bhatt P, Joseph GS, Negi PS, Varadaraj MC. Phenolic constituents and biological activities of leaf extract of traditional medicinal plant Plectranthus amboinicus Benth (Lamiaceae). TANG
2013;3(4):50-5.
16. Jasmine R, Selvi JS. Analysis of antibacterial activity of the bioactive constituents from the acetone extract of Coleus aromaticus (Benth)

Leaves by gas chromatography-mass spectrometry. World J Pharm
Pharm Sci 2013;2(5):3088-93.
17. Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants.
4th ed., Vol. 18. Madras: Orient Longman Limited; 1995. p. 315-7.
18. Gelmini F, Squillace P, Testa C, Sparacino AC, Angioletti S, Beretta G. GC-MS characterisation and biological activity of essential oils from different vegetative organs of Plectranthus barbatus and Plectranthus caninus cultivated in north Italy. Nat Prod Res
2015;29(11):993-8.
19. Sahaykhare R, Banerjee S, Kundu K. Coleus aromaticus Benth. - A nutritive medicinal plant of potential therapeutic value. Int J Pharm Biol Sci 2011;2(3):488-500.
20. Arslanyolu M, Erdemgil FZ. Evaluation of the antibacterial activity and toxicity of isolated arctin from the seeds of Centaurea sclerolepis. J Fac Pharm Ankara 2006;35(2):103-9.
21. Manjamalai A, Alexander T, Berlin Grace VM. Bioactive evaluation of the essential oil of Plectranthus amboinicus by GC-MS analysis and its role as a drug for microbial infections and inflammation. Int J Pharm Pharm Sci 2012;4(3):205-11.
22. Logarto Parra A, Silva Yhebra R, Guerra Sardiñas I, Iglesias Buela L.
Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 2001;8(5):395-400.
23. Syahmi AR, Vijayarathna S, Sasidharan S, Latha LY, Kwan YP, Lau YL, et al. Acute oral toxicity and brine shrimp lethality of Elaeis guineensis Jacq. (oil palm leaf) methanol extract. Molecules
2010;15(11):8111-21.
24. Sahqal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM. Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. Pharm Res
2010; (4):215-20.
25. De Oliveira BH, Nakashimab T, de Souza Filhoc JD, Frehse FL.
HPLC analysis of flavonoids in Eupatorium littorale. J Braz Chem Soc
2001;12(2):243-6.
26. Solanki SS, Selvanayagam M. Phytochemical screening and study of predictive toxicity of certain medicinal plants and extracts using brine shrimp. Herb Tech Ind 2013;10(1):1-4.
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How to Cite
Janakiraman, D., and P. Somasundaram. “ACTIVE PHENOLIC CONSTITUENTS AND BRINE SHRIMP LETHALITY ASSAY OF HYDROALCOHOLIC EXTRACT OF Plectranthus Amboinicus”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 186-9, doi:10.22159/ajpcr.2017.v10i2.15115.
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