ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM TERMINALIA CHEBULA OF EASTERN GHATS, TAMILNADU
Objective: Endophytic fungi live inside the higher plants, apparently without causing any harm to the hosts and its produce the secondary metabolites are potential antimicrobial activity. Terminalia chebula has been used in Ayurveda, Unani & Homeopathy medicine. In this study an isolate and identify the endophytic fungi from T. Chebula collected from pachamalai hills of the Eastern Ghats, Tamilnadu.
Methods: The plant materials were taken and first rinsed in running tap water to remove the dust and the other debris present in it. Segments of approximately 0.5 cm were cut in sterile lancet blades and surface sterilized by agitating in 70% ethanol (5s), followed by treatment with 4% NaOCl (90s) and then rinsed in sterile distilled water (10s).Thirty six (leaf, stem and fruit samples) segments from T.chebula plant are processed for the isolation of endophytic fungi.
Results: About 36 segments (12 segments of each part respectively) of the medicinal plant were screened for the isolation of the endophytic fungi. A total of 27 endophytic fungi was isolated and identified from medicinal plant T.chebula. The leaf segments showed a maximum repository for endophytic fungi than the other segments. Among the 27 endophytic fungi, the predominant endophytic fungi isolated belonged to the genera of Alternaria longipes, Curvularia spp, Mucor, phoma spp, Aspergillus niger, Aspergillus flavus and Penicillium spp. In this study majority of the fungi belonged to hyaline hyphomycetes.
Conclusion: In this study conclude that the isolation of endophytic fungi from medicinal plant of terminalia chebula. To isolate the 27 endophytic fungi produce the novel bioactive compound. However Further studies are required to screen these endophytic fungi for production of novel Bioactive compounds.
Keywords: Endophytic fungi, Terminalia chebula, bioactive compound
2. Vaidyanathan D, Senthilkumar MS, Sisubalan N, Basha MG. Studies on ethnomedicinal plants used by Malayali Gounder Tribes in Pachamalai of Eastern Ghats, Tamil Nadu, India. Adv Appl Sci Res 2014;5(1):244-53.
3. Dar RA, Rather SA, Mushtaq S, Qazi PH. Purification and characterization of endophytic fungal strains from four different high value medicinal plants of Kashmir valley. Int J Phytopharm 2015;5(1):8-11.
4. Sandhu SS, Kumar S, Aharwal RP. Isolation and identification of endophytic fungi from Ricinus communis Linn. and their antibacterial activity. Int J Res Pharm Chem 2014;4(3):611-8.
5. Shiva Kameshwari MN, Mohana B, Thara Saraswathi KJ. Isolation and identification of endophytic fungi from Urginea indica, a medicinal plant from diverse regions of South India. Int J Latest Res Sci Technol 2015;4(1):75-80.
6. Anitha D, Vijaya T, Pragathi D, Reddy NV, Mouli KC, Venkateswarulu N, et al. Isolation and characterization of endophytic fungi from endemic medicinal plants of Tirumala hills. Int J Life Sci Biotechnol Pharm Res 2013;2(3):367-73.
7. Divya CR, Sharada MS, Ashwini V. Isolation and identification of endophytic fungi in Trigonella foenum-graceum L. and their antibacterial activity. Imp J Interdiscip Res 2016;2(9):516-21.
8. Dhanalakshmi R, Umamaheswari S, Sugandhi P, Prasanth DA. Biodiversity of the endophytic fungi isolated from Moringa oleifera of Yercaud hills. Int J Pharm Sci Res 2013;4(3):1064-8.
9. Barnabas J, Murthy S, Jagdeesh S. Antimicrobial properties of endophytic fungi isolated from Cynodon dactylon and Moringa oleifera. Int J Biol Pharm Res 2013;4(2):98-104.
10. Tenguria RK, Firodiya A. Occurrence of endophytic fungi in leaves of medicinal plants from Central Region of Madhya Pradesh, India. World J Pharm Pharm Sci 2015;4(11):1921-34.
11. Job N, Manomi S, Philip R. Isolation and characterization of endophytic fungi from Avicennia officinalis. Int J Res Biomed Biotechnol 2015;5(1):4-8.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.