IN VITRO ANALYSIS OF ORNITHINE DECARBOXYLASE INHIBITORY ACTIVITY OF THE EXTRACTS OF EXCOECARIA AGALLOCHA L.
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.15698Abstract
Objective: Ornithine decarboxylase (ODC) is one of the key enzymes involved in polyamine synthesis that has a prominent regulatory role in cell growth in prokaryotes and eukaryotes. Its regulation/inhibition is a potential means of controlling cell growth. The purpose of the study was to check the effect of the methanol extracts of leaves and seeds of the mangrove plant Excoecaria agallocha in controlling/inhibiting ODC enzyme produced by Escherichia coli.
Methods: The methanol extracts of leaves and seeds of the mangrove plant E. agallocha were made. The inhibitory effect of the extracts on ODC enzyme produced by E. coli was tested by a modified spectrophotometric assay (measured in terms of µM of putrescine produced), and the antibacterial effect of the extract was determined by well diffusion assay.
Results: It was found that both the leaf and seed extracts inhibited ODC activity thereby preventing the conversion of ornithine to putrescine (the prominent by-product of ODC activity) in a concentration-dependent manner. The leaf extract exhibited 50% inhibition of ODC activity at a concentration of 17.5 µg/ml whereas seed extract inhibited 50% of the activity at a concentration of 16.1 µg/ml. In the growth inhibition assay by well diffusion method, the effect of the extracts on the growth of E. coli was studied and a concentration-dependent growth inhibition was found - 100 µl of leaf extract showed a zone of inhibition of 12.31 mm (diameter) and seed extract showed 12.64 mm, confirming the antibacterial activity of the extracts.
Conclusion: The ODC inhibition-mediated growth inhibition of E. coli is a novel finding as far as the extracts of E. agallocha are concerned. The extract was potent enough to be suggested as an antibacterial agent.
Keywords: Ornithine decarboxylase, Excoecaria agallocha, Escherichia coli, Ornithine decarboxylase inhibition, Putrescine, Ornithine, Antibacterial effect.
Downloads
References
Luqman S. Ornithine decarboxylase: A promising and exploratory candidate target for natural products in cancer chemoprevention. Asian Pac J Cancer Prev 2012;13(5):2425-7.
Bachmann AS, Patil SS. Characterization of ornithine decarboxylase from Pseudomonas syringae pv. Phaseolicola and its inhibition by phaseolotoxin. Physiol Mol Plant Pathol 2003;63(2):57-63.
Badolo L, Berlaimont V, Helson-Cambier M, Hanocq M, Dubois J.
Simple and rapid enzymatic assay of ornithine decarboxylase activity. Talanta 1999;48(1):127-34.
Wei H, Tye L, Bresnick E, Birt DF. Inhibitory effect of apigenin, a plant flavonoid, on epidermal ornithine decarboxylase and skin tumor promotion in mice. Cancer Res 1990;50(3):499-502.
Yin BW, Shen LR, Zhang ML, Zhao L, Wang YL, Huo CH, et al.
Chemical constituents of plants from the genus Excoecaria. Chem
Biodivers 2008;5(11):2356-71.
Taylor WI. Isolation of Salmonellae from food samples. V.
Determination of the method of choice for enumeration of Salmonella. Appl Microbiol 1961;9:487-90.
Choi H, Kyeong HH, Choi JM, Kim HS. Rational design of ornithine decarboxylase with high catalytic activity for the production of putrescine. Appl Microbiol Biotechnol 2014;20:1-8.
Luqman S, Masood N, Srivastava S, Dubey V. A modified spectrophotometric and methodological approach to find novel inhibitors of ornithine decarboxylase enzyme: A path through the maze. Protoc Exch 2013. DOI:10.1038/protex.2013.045.
Jänne J, Williams-Ashman HG. On the purification of L-ornithine decarboxylase from rat prostate and effects of thiol compounds on the enzyme. J Biol Chem 1971;246(6):1725-32.
Schenkel E, Berlaimont V, Dubois J, Helson-Cambier M, Hanocq M.
Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: Application to P388 cancer cells. J Chromatogr B Biomed Appl
;668(2):189-97.
Maderdrut JL, Oppenheim RW. A radiometric microassay for ornithine decarboxylase. Neuroscience 1978;3(6):587-94.
Sharma S, Stutzman JD, Garris KR, Steele VE. An ornithine decarboxylase activity assay to screen for potential chemopreventive agents. Methods Cell Sci 1997;19:33-6.
Minocha R, Long ST, Makib H, Minocha SC. Assays for the activities of polyamine biosynthetic enzymes using intact tissues. Plant Physiol Biochem 1999;37:597-603.
Tilak R, Punit K, Radha R, Kashyap KD. Screening of some medicinal plants for their antimicrobial activities. Int J Pharm Pharm Sci
;8(5):202-6.
Davidson NE, Hahm HA, McCloskey DE, Woster PM, Casero RA Jr.
Clinical aspects of cell death in breast cancer: The polyamine pathway as a new target for treatment. Endocr Relat Cancer 1999;6(1):69-73.
Duggi S, Handral HK, Handral R, Tulsianand G, Shruth SD. Turmeric: Nature’s precious medicine. Asian J Pharm Clin Res 2013;6(3):10-6.
Ramirez-Mares MV, Chandra S, de Mejia EG. In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols. Mutat Res 2004;554(1-2):53-65.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
