ANTICANCER ACTIVITY OF CAMELLIA SINENSIS MEDIATED COPPER NANOPARTICLES AGAINST HT-29, MCF-7 AND MOLT-4 HUMAN CANCER CELL LINES
Objective: With the advancement in nanotechnology, it is imperative to unearth its applications in medicine. Present investigation deals with the copper nanoparticles biosynthesizing capability of the leaves of medicinally important plant,Â Camellia sinensis.
Methods: The phytosynthesized CuNPs were characterized by EDX, NTA, XRD, SEM, TEM and FTIR analysis. In the current study, we have made attempts to exploit the anticancer ability of the copper nanoparticles against HT-29 colon cancer, MCF 7 breast cancer and MOLT-4 leukemia cancer cell lines via SRB assay. We also carried out the synergistic activity with standard drug Adriamycin (ADR).
Results: The synthesis of CuNPs was confirmed using EDX analysis where the presence of a strong optical absorption peak was observed at 1 keV, which is typical for the absorption of metallic copper nanoparticles. According to the results obtained, CuNPs showed good antiproliferative results in a dose dependant manner on HT-29 and MCF-7 cell lines, with 80 Âµg/ml concentration giving the best result. The synergistic effect of CuNPs+ADR was even better than that of CuNPs alone on all the cell lines. The synergism drug combinations showed highly responsive results on the leukemia cell line compared to individual drugs.
Conclusion: Among all the treatments and cell lines studied, the most favorable and responsive antiproliferative impact was recorded for CuNPs+ADR combination treatment at 40Âµg/ml concentration on MCF-7 breast cancer cell line.
Keywords: CuNPs, Camellia sinensis, Anticancer, Sulforhodamine B assay, HT-29, MCF-7, MOLT-4.
Gerber LC, et al. Nanoparticle cytotoxicity depends on intracellular
solubility: Comparison of stabilized copper metal and degradable
copper oxide nanoparticles. Toxicol Lett 2010;197(3):169-74.
2. Rajendran R, Ganesan N, Balu SK, Alagar S, Thandavamoorthy P,
Thiruvengadam D. Green synthesis, characterization, antimicrobial
and cytotoxic effects of silver nanoparticles using Origanum
heracleoticum L. Leaf extract. Int J Pharm Pharm Sci 2015;7(4):288-93.
3. Burrows CJ, Muller JG. Oxidative nucleobase modifications leading to
strand scission. Chem Rev 1998;98(3):1109-52.
4. Zhang Y, Aslan K, Previte MJ, Geddes CD. Plasmonic engineering
of singlet oxygen generation. Proc Natl Acad Sci U S A
5. Kharat SN, Mendhulkar VD. Synthesis, characterization and studies on
antioxidant activity of silver nanoparticles using Elephantopus scaber
leaf extract. Mater Sci Eng C Mater Biol Appl 2016;62:719-24.
6. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for
cytotoxicity screening. Nat Protoc 2006;1(3):1112-6.
7. Caroling G, Vinodhini E, Ranjitham AM, Shanthi P. Biosynthesis of copper nanoparticles using aqueous Phyllanthus emblica (gooseberry) extract- characterisation and study of antimicrobial effects. Int J Nanomater Chem 2015;1(2):53-63.
8. Senthilkumar SR, Sivakumar T. Green tea (Camellia sinensis) mediated
synthesis of zinc oxide (ZnO) nanoparticles and studies on their
antimicrobial activities. Int J Pharm Pharm Sci 2014;6(6):461-5.
9. Joshi UJ, Gadge AS, Dâ€™Mello P, Sinha R, Srivastava S, Govil G. Antiinflammatory, antioxidant and anticancer activity
of quercetin and its analogues.
Int J Res Pharm Biomed Sci 2011;2(4):1756-66.
10. Sankar R, Maheswari R, Karthik S, Shivashangari KS, Ravikumar V.
Anticancer activity of Ficus religiosa engineered copper oxide
nanoparticles. Mater Sci Eng C Mater Biol Appl 2014;44:234-9.
11. Nagajyothi PC, Muthuraman P, Sreekanth TV, Doo HK, Jaesool S. Green
synthesis: In-vitro anticancer activity of copper oxide nanoparticles
against human cervical carcinoma cells. Arabian J Chem 2016; In Press. Available from: http://www.dx.doi.org/10.1016/j.arabjc.2016.01.011.
12. Badawi AM, Zakhary NI, Morsy SM, Sabry GM, Mohamed MR, Mousa AM.Copper (II)-surfactant complex and its nano analog as potential antitumor agents. J Dispers Sci Technol 2009;30(9):1303-9.
13. Chen C, Shen G, Hebbar V, Hu R, Owuor ED, Kong AN.
Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells. Carcinogenesis 2003;24(8):1369-78.
14. Yang CS, Wang ZY. Tea and cancer. J Natl Cancer Inst
15. Baskar G, Sathivel K, George GB. In vitro cytotoxicity of copper oxide
nanobiocomposites synthesized by Catharanthus roseus flower extract against breast cancer cell line. J Chem Pharm Sci 2016;9(1):211-4.
16. Sivaraj R, Rahman PK, Rajiv P, Narendhran S, Venckatesh R.
Biosynthesis and characterization of Acalypha indica mediated copper
oxide nanoparticles and evaluation of its antimicrobial and anticancer
activity. Spectrochim Acta A Mol Biomol Spectrosc 2014;129:255-8.
17. KonarikovÃ¡ K, JeÅ¾ovicovÃ¡ M, KeresteÅ¡ J, GbelcovÃ¡ H, DurackovÃ¡ Z,
Å½itnanovÃ¡ I. Anticancer effect of black tea extract in human cancer cell
lines. Springerplus 2015;4:127.
18. Han DH, Jeong JH, Kim JH. Anti-proliferative and apoptosis induction
activity of green tea polyphenols on human prom
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