QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article
Objectives: To analyze the phytochemicals quantitatively and screening the antioxidant efficacy of methanol extracts of Elytraria acaulis plant extract
by in vitro.
Methods: The total phenols, flavonoids, and tannin contents analyzed by Folin-Ciocalteu method, aluminum chloride colorimetric and calculated as
gallic acid equivalents/g (GAE/g) of dry weight. Free radical scavenging activity was screened using 1,1-diphenyl-2-picrylhydrazyl (DPPH), radical
scavenging activities, and ferric reducing power assay was also analyzed.
Results: The total phenolic content of the aqueous leaf extract was 46.84 Âµg GAE/g of extract powder. Significantly similar levels of total flavonoid and
tannin contents of the plant were 41.72 and 39.50 Âµg GAE/g, respectively. The plant extracts showed appreciable free radical scavenging activities at
the highest concentration of 400 Âµg/mL superoxide anion radical with IC
values (107.7Â±1.081 Âµg/mL), and for DPPH and considerably high amount
of radical scavenging activity was found with very low IC
values (4.3Â±0.88 Âµg/mL) compared with quercetin.
Conclusion: The phenolic and flavonoid compounds provide substantial antioxidant properties which could be effectively used for pharmaceutical,
nutraceutical as well as anti-inflammatory applications.
Keywords: Phenols, Flavonoids, 1,1-diphenyl-2-picrylhydrazyl, Pharmaceuticals.
Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and Extraction: A Review. Int Pharma Sciencia 2011; 1(1): 98-106.
Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction Technologies for Medicinal and Aromatic Plants. Int centre for science and high tech, Trieste, 2008; 21-25.
Nikhal SB, Dambe PA, Ghongade DB, Goupale DC. Hydroalcoholic extraction of Mangifera indica (leaves) by Soxhletion. Int J of Pharma Sciences 2010; 2 (1): 30-32.
Moskovitz J, Yim KA, Choke PB. Free radicals and disease. Arch Biochem Biophys 2002; 397: 354â€“359.
Chessbrough M. Medical Laboratory Manual for Tropical Countries, pp.260 in Linacre House, Jordan Hill, Oxford 2000.
Singleton, V., R. Orthofer and R. Lamuela-Ravento. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin - Ciocalteu reagent. In L. Packer (Ed.). Oxidants and antioxidants, part A. Methods in enzymology 1999; 299: 152-178.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food and Drug Analysis 2002; 10: 178-182.
Siddhuraju P and S. Manian, The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds, Food Chem 2007; 105: 950â€“958.
Saha, K., Lajis, N. H., Israf, D. A., Hamzah, A.S., Khozirah, S., Khamis, S., Syahida, A. (2004). Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J of Ethnopharmac. 92, 263-267.
Nishikimi M, Appaji N, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res communication 1972; 46: 849 - 854.
Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44:307â€“315.
Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008; 8(7): 634-646.
Khacha Ananda S, Tragoolpua K, Chantawannakul P, Tragoolpua Y. Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods. Asian Pac J Cancer Prev 2013; 14: 6991-6995.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006; 5(11): 1142-1145.
Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009; 22(1): 102-106.
Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci 2011; 12: 3422-3431.
Nayak BS, Pinto Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complement Altern Med 2006; 6: 41.
Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets 2009; 8(3): 229-235.
Soobrattee. M.A, V.S. Neergheen, A. Luximon-Ramma, O.I. Aruoma, O.T. Bahorun. Phenolics as potential antioxidant therapeutic agents: mechanism and actions, Mutat Res Fundam Mol 2005; 579: 200â€“213.
Shukla. S, A. Mehta, K.V. Bajpai, S. Shukla. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem Toxicol 2009; 47: 2338â€“2343.
Karthikeyan Jagadeesan, Thooyavan George, Mullai Nila Kamalanathan, Ilayaraja T. Studies on the Anthelmintic and Antioxidant Activity of Root Extracts of E.acaulis. Advances in animal and veterinary sciences August 2014; 2: 8457-463.
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