QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article

Abstract

ABSTRACT
Objectives: To analyze the phytochemicals quantitatively and screening the antioxidant efficacy of methanol extracts of Elytraria acaulis plant extract
by in vitro.
Methods: The total phenols, flavonoids, and tannin contents analyzed by Folin-Ciocalteu method, aluminum chloride colorimetric and calculated as
gallic acid equivalents/g (GAE/g) of dry weight. Free radical scavenging activity was screened using 1,1-diphenyl-2-picrylhydrazyl (DPPH), radical
scavenging activities, and ferric reducing power assay was also analyzed.
Results: The total phenolic content of the aqueous leaf extract was 46.84 µg GAE/g of extract powder. Significantly similar levels of total flavonoid and
tannin contents of the plant were 41.72 and 39.50 µg GAE/g, respectively. The plant extracts showed appreciable free radical scavenging activities at
the highest concentration of 400 µg/mL superoxide anion radical with IC
values (107.7±1.081 µg/mL), and for DPPH and considerably high amount
of radical scavenging activity was found with very low IC
50
50
values (4.3±0.88 µg/mL) compared with quercetin.
Conclusion: The phenolic and flavonoid compounds provide substantial antioxidant properties which could be effectively used for pharmaceutical,
nutraceutical as well as anti-inflammatory applications.
Keywords: Phenols, Flavonoids, 1,1-diphenyl-2-picrylhydrazyl, Pharmaceuticals.

Author Biography

Manigandan m, Annamalai University

Ph.D., Research Scholar, Senior Research Fellow in UGC - MRP. Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar.

References

1. Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and Extraction: A Review. Int Pharma Sciencia 2011; 1(1): 98-106.
2. Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction Technologies for Medicinal and Aromatic Plants. Int centre for science and high tech, Trieste, 2008; 21-25.
3. Nikhal SB, Dambe PA, Ghongade DB, Goupale DC. Hydroalcoholic extraction of Mangifera indica (leaves) by Soxhletion. Int J of Pharma Sciences 2010; 2 (1): 30-32.
4. Moskovitz J, Yim KA, Choke PB. Free radicals and disease. Arch Biochem Biophys 2002; 397: 354–359.
5. Chessbrough M. Medical Laboratory Manual for Tropical Countries, pp.260 in Linacre House, Jordan Hill, Oxford 2000.
6. Singleton, V., R. Orthofer and R. Lamuela-Ravento. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin - Ciocalteu reagent. In L. Packer (Ed.). Oxidants and antioxidants, part A. Methods in enzymology 1999; 299: 152-178.
7. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food and Drug Analysis 2002; 10: 178-182.
8. Siddhuraju P and S. Manian, The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds, Food Chem 2007; 105: 950–958.
9. Saha, K., Lajis, N. H., Israf, D. A., Hamzah, A.S., Khozirah, S., Khamis, S., Syahida, A. (2004). Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J of Ethnopharmac. 92, 263-267.
10. Nishikimi M, Appaji N, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res communication 1972; 46: 849 - 854.
11. Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44:307–315.
12. Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008; 8(7): 634-646.
13. Khacha Ananda S, Tragoolpua K, Chantawannakul P, Tragoolpua Y. Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods. Asian Pac J Cancer Prev 2013; 14: 6991-6995.
14. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006; 5(11): 1142-1145.
15. Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009; 22(1): 102-106.
16. Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci 2011; 12: 3422-3431.
17. Nayak BS, Pinto Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complement Altern Med 2006; 6: 41.
18. Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets 2009; 8(3): 229-235.
19. Soobrattee. M.A, V.S. Neergheen, A. Luximon-Ramma, O.I. Aruoma, O.T. Bahorun. Phenolics as potential antioxidant therapeutic agents: mechanism and actions, Mutat Res Fundam Mol 2005; 579: 200–213.
20. Shukla. S, A. Mehta, K.V. Bajpai, S. Shukla. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem Toxicol 2009; 47: 2338–2343.
21. Karthikeyan Jagadeesan, Thooyavan George, Mullai Nila Kamalanathan, Ilayaraja T. Studies on the Anthelmintic and Antioxidant Activity of Root Extracts of E.acaulis. Advances in animal and veterinary sciences August 2014; 2: 8457-463.
Statistics
536 Views | 534 Downloads
Citatons
How to Cite
m, M., and K. K. “QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 9, Dec. 2016, doi:10.22159/ajpcr.2016.v9s3.15759.
Section
Original Article(s)