QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article

Manigandan m; Kolanjinathan K

doi:10.22159/ajpcr.2016.v9s3.15759

Authors

Manigandan m Annamalai University http://orcid.org/0000-0002-8115-2713
Kolanjinathan K

DOI:

https://doi.org/10.22159/ajpcr.2016.v9s3.15759

Abstract

ABSTRACT
Objectives: To analyze the phytochemicals quantitatively and screening the antioxidant efficacy of methanol extracts of Elytraria acaulis plant extract
by in vitro.
Methods: The total phenols, flavonoids, and tannin contents analyzed by Folin-Ciocalteu method, aluminum chloride colorimetric and calculated as
gallic acid equivalents/g (GAE/g) of dry weight. Free radical scavenging activity was screened using 1,1-diphenyl-2-picrylhydrazyl (DPPH), radical
scavenging activities, and ferric reducing power assay was also analyzed.
Results: The total phenolic content of the aqueous leaf extract was 46.84 Âµg GAE/g of extract powder. Significantly similar levels of total flavonoid and
tannin contents of the plant were 41.72 and 39.50 Âµg GAE/g, respectively. The plant extracts showed appreciable free radical scavenging activities at
the highest concentration of 400 Âµg/mL superoxide anion radical with IC
values (107.7Â±1.081 Âµg/mL), and for DPPH and considerably high amount
of radical scavenging activity was found with very low IC
50
50
values (4.3Â±0.88 Âµg/mL) compared with quercetin.
Conclusion: The phenolic and flavonoid compounds provide substantial antioxidant properties which could be effectively used for pharmaceutical,
nutraceutical as well as anti-inflammatory applications.
Keywords: Phenols, Flavonoids, 1,1-diphenyl-2-picrylhydrazyl, Pharmaceuticals.

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Author Biography

Manigandan m, Annamalai University

Ph.D., Research Scholar, Senior Research Fellow in UGC - MRP. Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar.

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