• Saranya D
  • Sekar J
  • ADAIKALA Raj G Annamalai universityDepartment of botany


Objective: This study evaluated the antioxidant properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Abutilon indicum (L).

Methods: The different extracts of A. indicum leaves, bark and roots  were antioxidant potential by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2-Azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS•+) , Hydrogen peroxide scavenging, Superoxide anions scavenging,  Hydroxyl radical scavenging, Ferric reducing antioxidant power, Total antioxidant activity (Phosphomolybdic acid) and total phenol and flavonoid contents.                      

Results: The highest total phenol and flavonoid contents in leaves extracts of A. indicum. The total phenol (3.08 ± 0.06) mg/ml and flavonoid (7.16 ± 0.15) mg/ml were found to be higher in ethyl acetate extract of A. indicum and free radical scavenging activities IC50 values were calculated and compare to standard for L- ascorbic acid, (BHT) butylated hydroxytoluene and gallic acid    Conclusion:  The results of the study revealed that the ethyl acetate extract of A. indicum leaves can be used for the biological characterization and importance of the compounds identified and creates a platform to screen many bioactive compounds to treat many diseases.

Keywords: Abutilon indicum, Free radical scavenging activity, 1,1-diphenyl-2-picryl-hydrazyl, 2-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid,
Ferric reducing antioxidant power.


1. Tiwari AK. Antioxidants: New-generation therapeutic base for treatment of polygenic disorders. Curr Sci 2004;86(8):1092-102.
2. Sivanandham V. Free radicals in health and diseases. Pharmacol Online 2011;1:1062-77.
3. Shinde A, Ganu J, Naik P. Effect of free radicals and antioxidants on oxidative stress. J Dent Allied Sci 2012;1(2):63-6.
4. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39(1):44-84.
5. Ferreira IC, Barros L, Abreu RM. Antioxidants in wild mushrooms. Curr Med Chem 2009;16(12):1543-60.
6. Cavar S, Kovac F, Maksimovic M. Evaluation of the antioxidant activity of a series of 4-methylcoumarins using different testing methods. Food Chem 2012;133:930-7.
7. Singh R, Baichheti R, Saini CK, Singh U. In vitro antioxidant activity of Canna indica extracts using different solvent system. Asian J Pharm Clin Res 2016;9(6):1-4.
8. Vinoth B, Manivasagaperumal R, Prakash P. Free radical scavenging potential of different extracts from Azima tetracantha Lam. Int J Res Ayurveda Pharm 2015;6(1):131-7.
9. Porchezhian E, Ansari SH. Effect of liquid extract from fresh Abutilon indicum leaves and Allium cepa bulbs on paracetamol and carbontetrachloride induced hepatotoxicity. Pharmazie 2000;55(9):702-3.
10. Anyensu ES. Medicinal Plants of West Africa. Algonac, Michigan: Publications Inc.; 1978. p. 1-10.
11. Lakshmayya S, Nelluri NR, Kumar P, Agarwal NK, Gouda TS, Setty SR. Phytochemical and pharmacological evaluation of leaves of Abutilon indicum. Indian J Tradit Knowl 2003;2(1):79-83.
12. Afolayan AJ. Extracts from the shoots of Arctotis artotoides inhibit the growth of bacteria and fungi. Pharm Biol 2003;41:22-5.
13. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;29:1199-200.
14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.
15. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10(6):1003-8.16. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975;85(2):337-41.
17. Elizabeth K, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58(13):237-40.
18. Prieto P, Pineda M, Aguilar M. Spectrosphotometric quantization of antioxidant capacity through the formation of a phospomolybdenum complex: Specific application to the determination of Vitamin E. Anal Biochem 1999;269(2):337-41.
19. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. J Nutr 1986;44:307-15.
20. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
21. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
22. Menaga D, Rajakumar S, Ayyasamy PM. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int J Pharm Pharm Sci 2013;5(4):601-6.
23. Nayak R, Panda A, Samanta L, Sahoo S. Phytochemical analysis of antioxidants from Abutilon indicum L. and Paederia foetida L. Supported by TLC, FTIR and NMR studies. Int J Sci Res 2015;4(5):2319-24.
24. Kumar PM, Sasmal D, Mazumder PM. The antihyperglycemic effect of aerial parts of Salvia splendens (scarlet sage) in streptozotocin-induced diabetic-rats. Pharmacognosy Res 2010;2(3):190-4.
25. Jamuna BA, Ravishankar RV. In-vitro evaluation of antioxidant and antibacterial activities of Rotula aquatica and Ancistrocladus heyneanus: Antioxidant and antimicrobial activity of medicinal plants. J Pharm Res 2013;6(2):313-7.
26. Whistler RL, BeMiller JN. Industrial Gums: Polysaccharides and Their Derivatives. London, UK: Academic Press; 1993.
27. Chen K, Plumb GW, Bennett RN, Bao Y. Antioxidant activities of extracts from five anti-viral medicinal plants. J Ethnopharmacol 2005;96(1-2):201-5.
28. Surveswaran S, Cai Y, Corke H, Sun M. Phytochemical screening and biological activities of the oil components of Prunus domestica. Food Chem 2007;102:938-53.
29. Shirwaikar A, Punitha IS. Antioxidant studies on the methanol stem extract of Coscinium fenestratum. Natural Product Sci 2007;13(1):40-5.
30. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49(11):5165-70.
31. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature 2000;408(6809):239-47.
32. Yasmin S, Kashmiri MA, Ahmad I, Adnan A, Ahmad M. Chemical constituents of Abutilon indicum Linn. Pharm Biol 2008;46:673-6.
33. Ahmed F, Urooj A. Glucose-lowering, hepatoprotective and hypolipidemic activities of stem bark of Ficus racemosa in streptozotocin induced diabetic rats. J Young Pharm 2009;1:160-4.
34. Ashafa AO, Grierson DS, Afolayan AJ. In vitro antioxidant activity of extracts from the leaves of Felicia muricata thumb an underutilized medicinal plants in the Eastern Cape, Province of South Africa. Afr J Tradit Complement Altern Med 2010;7:294-302.
35. Archana I, Vijayalakshmi K. Preliminary phytochemical screening and in vitro free radical scavenging activity of root extracts of Glycyrrhiza glabra L. Asian J Pharm Clin Res 2016;9(6):1-6.
36. Lie FS, Jieh HT, Je HC, Chih YC, Chiu PL. Antioxidant properties of extracts from medicinal plants popularly used in Taiwan. Int J Appl Sci Eng 2005;3(3):195-202.
37. Dhruti N. Antioxidant activities of methanolic and aqueous extracts from leaves of Martynia annua Linn. Pharmacognosy J 2009;1(4):288-90.
38. Hatano T, Edamatsu R, Mori A. Effects of interaction of tannins with coexisting substances. Chem Pharm Bull 1989;37:2016-21.
39. Sawant O, Kadam J, Ghosh R. In-vitro free radical scavenging and antioxidant activity of Adiantum lunulactum. J Herb Med Toxicol 2009;3:39-44.
40. Stankovic MS, Neda N, Topuzovic M, Solujic S. Total phenolic content, flavonoid concentrations and antioxidant activity, of the whole plant and plant parts extracts from Teucrium montanum L. Var. montanum, F. Supinum (L.) Reichenb. Biotechnol Biotechnol Equip 2011;25(1):2222-7.
41. Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992;32:67-103.
42. Tosun M, Ercisli S, Sengul M, Ozer H, Polat T, Ozturk E. Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biol Res 2009;42(2):175-81.
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How to Cite
D, S., S. J, and A. Raj G. “ASSESSMENT OF ANTIOXIDANT ACTIVITIES, PHENOL AND FLAVONOID CONTENTS OF DIFFERENT EXTRACTS OF LEAVES, BARK AND ROOT FROM THE Abutilon Indicum (L.) SWEET”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 88-94, doi:10.22159/ajpcr.2017.v10i4.15884.
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