ANTI- OBSESSIVE COMPULSIVE ACTIVITY OF HONEY
Objective: The present study aim to investigate the anti-obsessive compulsive disorder potential of honey in mice.
Methods: The honey was given orally in two concentrations of 17.5 ml/kg and 35 ml/kg to mice for 21 days. The anti-obsessive compulsive activity was assessed on 21th day by in-vitro methods viz flickering-light induced obsessive- compulsive behaviour model developed in our laboratory (Patent No. 3087/DEL/2012) and marble-burying behaviour model. The biochemical estimation was also done on 21 days.
Results: When honey was administered chronically for 21 days significantly reduced gnawing behaviour and marble-burying behaviour of mice. Interestingly in our biochemical estimations, both, brain serotonin and GABA level were significantly increased by honey. The anti-Obsessive compulsive activity of honey may be due to the presence of Tryptophan, which is an important precursor of serotonin in the serotonergic neurons thereby enhancing the biosynthesis of serotonin to facilitate the anti- obsessive compulsive activity.
Conclusion: The present study revealed that honey possessed significant anti-OCD activity.
2. Singh MP, Chourasia HR, Agarwal M, Malhotra A, Sharma S, Sharma D, et al. Honey as complementary medicine: A review. Int J Pharm Bio Sci 2012;3(2):12-31.
3. Boileau B. A review of obsessive-compulsive disorder in children and adolescents. Dialogues Clin Neurosci 2011;13(4):401-11.
4. Parle M, Rana T. How to test medicines of obsessive â€“ Compulsive disorder? Int Res J Pharm 2012;3:1-6.
5. Deacon RM. Digging and marble burying in mice: Simple methods for in vivo identification of biological impacts. Nat Protoc 2006;1(1):122-4.
6. Schlumpf M, Lichtensteiger W, Langemann H, Waser PG, Hefti F. A fluorometric micromethod for the simultaneous determination of serotonin, noradrenaline and dopamine in milligram amounts of brain tissue. Biochem Pharmacol 1974;23(17):2437-46.
7. Tamboli AM, Rub RA, Ghosh P, Bodhankar SL. Antiepileptic activity of lobeline isolated from the leaf of Lobelia nicotianaefolia and its effect on brain GABA level in mice. Asian Pac J Trop Biomed 2012;2(7):537-42.
8. Parle M, Gaikwad U. Clinical features of obsessive-compulsive disorder. Int Res J Pharm 2009;2:84-6.
9. Girdhar S, Wanjari MM, Prajapati SK, Girdhar A. Evaluation of anti- compulsive effect of methanolic extract of Benincasa hispida Cogn. Fruit in mice. Acta Pol Pharm 2010;67(4):417-21.
10. Madhura TK. Role of oxidative stress in the pathogenesis of OCD. Biochem Anal Biochem 2015;4:1-7.
11. Fava L, Bellantuono S, Bizzi A, Cesario ML, Costa B, De Simoni E, et al. Review of obsessive compulsive disorders theories. Glob J Epidemiol Public Health 2014;1:1-13.
12. Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: Its medicinal and pharmacological applications. Afr J Pure Appl Chem 2010;4(8):142-51.
13. Kale MA, Bindu M, Khadkikar P. Role of antioxidants and nutrition in oxidative stree: a review. Int J App Pharm, 2015;7: 1-4.
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