RESPONSES OF BRAIN TISSUES AGAINST HYPOXIC CONDITION IN HEMORRHAGIC STROKE PATIENTS: NEUROGLOBIN EXPRESSION IN BRAIN TISSUE AND PLASMA
Objective: Strokes remain a significant health concern and are the highest cause of mortality and physical or mental disability in productive and the elderly hospitalized patients in Indonesia. Neuroglobin (Ngb) mostly located in the central and peripheral nervous system, predicted enhanced neuronal survival under hypoxic condition, such as in a stroke. The aim of this study is to observe the response of the brain tissue of hemorrhagic stroke patients against hypoxic/ischemic conditions. The objectives are to recognize the pattern of Ngb expression in the brain tissue and plasma of hemorrhagic stroke patients, and furthermore, to compare the level of Ngb in the brain tissue and plasma of hemorrhagic stroke patients.
Methods: This is an observational study with consecutive sampling methods using cerebral cortex and the blood of hemorrhagic stroke patients, who underwent craniotomies to evacuate hematomas at Cipto Mangunkusumo Hospital (RSCM) and other hospitals in Jakarta. Ngb expression was measured in brain tissue and blood using real time reverse transcription polymerase chain reaction, while the ELISA method was adopted to measure Ngb protein in plasma.
Results: Hypoxia/ischemia in the brain tissue of hemorrhagic stroke patients increased the expression of Ngb in brain tissue compared to the blood. The level of Ngb protein in plasma of hemorrhagic stroke patients increased significantly compared to normal subjects; however, there is no significant difference between the plasma and brain tissue of hemorrhagic stroke patients.
Conclusion: Hypoxia/ischemia in hemorrhagic stroke patients increases the expression of Ngb mRNA and protein level.
Keywords: Neuroglobin, Hypoxia, Hemorrhagic stroke.
2. Prince SA, Wilson LM. Patofisiologi: Konsepklinis Proses-Proses
Penyakit. 6th ed. Jakarta: EGC; 2006. p. 1119-20.
3. Burmester T, Hankeln T. Neuroglobin: A respiratory protein of the nervous system. News Physiol Sci 2004;19:110-3.
4. Sun Y, Jin K, Mao XO, Zhu Y, Greenberg DA. Neuroglobin is
up-regulated by and protects neurons from hypoxic-ischemic injury. Proc Natl Acad Sci U S A 2001;98(26):15306-11.
5. Burmester T, Weich B, Reinhardt S, Hankeln T. A vertebrate globin expressed in the brain. Nature 2000;407(6803):520-3.
6. Garry DJ, Mammen PP. Neuroprotection and the role of neuroglobin. Lancet 2003;362(9381):342-3.
7. Brunori M, GiuffrÃ¨ A, Nienhaus K, Nienhaus GU, Scandurra FM, Vallone B. Neuroglobin, nitric oxide, and oxygen: Functional pathways and conformational changes. Proc Natl Acad Sci U S A
8. Herold S, Fago A, Weber RE, Dewilde S, Moens L. Reactivity studies of the Fe(III) and Fe(II)NO forms of human neuroglobin reveal a potential role against oxidative stress. J Biol Chem 2004;279(22):22841-7.
9. Bunn HF, Poyton RO. Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev 1996;76(3):839-85.
10. Wang X, Liu J, Zhu H, Tejima E, Tsuji K, Murata Y, et al. Effects of neuroglobin overexpression on acute brain injury and long-term outcomes after focal cerebral ischemia. Stroke 2008;39(6):1869-74.
11. Jin K, Mao Y, Mao X, Xie L, Greenberg DA. Neuroglobin expression in ischemic stroke. Stroke 2010;41(3):557-9.
12. Zhao S, Chu Z, Ma L, Chen Y, Wang L, Wang B, et al. Significance of neuroglobin in serum of acute atherosclerotic cerebral infarction patients. Neural Regen Res 2011;6(27):2140-5.
13. Zhang W, Tian Z, Sha S, Cheng LY, Philipsen S, Tan-Un KC. Functional and sequence analysis of human neuroglobin gene promoter region. Biochim Biophys Acta 2011;1809(4-6):236-44.
14. Hardiany NS, Wanandi SI. Correlation between oxidative stress and tumor grade in glioma cells from patients in Jakarta. Med J Indones
15. Shang A, Zhou D, Wang L, Gao Y, Fan M, Wang X, et al. Increased neuroglobin levels in the cerebral cortex and serum after ischemia- reperfusion insults. Brain Res 2006;1078(1):219-26.
16. Paulbabu K, Singh KD, Prashanti P, Padmaja M. Neuroprotective potential and efficacy of neurodegenerative disorders of fruit extract of Aegle marmelos. Int J Pharm Pharm Sci 2014;7(1):155-9.
17. Dirnagl U, Iadecola C, Moskowitz MA. Pathobiology of ischaemic stroke: An integrated view. Trends Neurosci 1999;22(9):391-7.
18. Casado B, Pannell LK, Whalen G, Clauw DJ, Baraniuk JN. Human neuroglobin protein in cerebrospinal fluid. Proteome Sci 2005;3(1):2.
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